scholarly journals Intrahaplotype Polymorphism at the Brassica S Locus

Genetics ◽  
2001 ◽  
Vol 159 (2) ◽  
pp. 811-822
Author(s):  
Christine Miege ◽  
Véronique Ruffio-Châble ◽  
Mikkel H Schierup ◽  
Didier Cabrillac ◽  
Christian Dumas ◽  
...  
Keyword(s):  
Class Ii ◽  
The Self ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
S Haplotype

Abstract The S locus receptor kinase and the S locus glycoproteins are encoded by genes located at the S locus, which controls the self-incompatibility response in Brassica. In class II self-incompatibility haplotypes, S locus glycoproteins can be encoded by two different genes, SLGA and SLGB. In this study, we analyzed the sequences of these genes in several independently isolated plants, all of which carry the same S haplotype (S2). Two groups of S2 haplotypes could be distinguished depending on whether SRK was associated with SLGA or SLGB. Surprisingly, SRK alleles from the two groups could be distinguished at the sequence level, suggesting that recombination rarely occurs between haplotypes of the two groups. An analysis of the distribution of polymorphisms along the S domain of SRK showed that hypervariable domains I and II tend to be conserved within haplotypes but to be highly variable between haplotypes. This is consistent with these domains playing a role in the determination of haplotype specificity.

Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.

2019 ◽  
Vol 51 (7) ◽  
pp. 723-733 ◽  
Author(s):  
Songmei Shi ◽  
Qiguo Gao ◽  
Tonghong Zuo ◽  
Zhenze Lei ◽  
Quanming Pu ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Signaling Pathway ◽  
Chromosomal Region ◽  
The Self ◽  
Mature Stage ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Phylogenic Tree ◽  
Brassica Oleracea L

Abstract Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway.

scholarly journals Site-Specific N-Glycosylation of the S-Locus Receptor Kinase and Its Role in the Self-Incompatibility Response of the Brassicaceae

2014 ◽  
Vol 26 (12) ◽  
pp. 4749-4762 ◽  
Author(s):  
Masaya Yamamoto ◽  
Titima Tantikanjana ◽  
Takeshi Nishio ◽  
Mikhail E. Nasrallah ◽  
June B. Nasrallah
Keyword(s):  
The Self ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Site Specific

scholarly journals The Self-Incompatibility Phenotype in Brassica Is Altered by the Transformation of a Mutant S Locus Receptor Kinase

1998 ◽  
Vol 10 (2) ◽  
pp. 209-218 ◽  
Author(s):  
Richard J. Stahl ◽  
MaryAnne Arnoldo ◽  
Tracy L. Glavin ◽  
Daphne R. Goring ◽  
Steven J. Rothstein
Keyword(s):  
The Self ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus

S-locus receptor kinase signalling

2014 ◽  
Vol 42 (2) ◽  
pp. 313-319 ◽  
Author(s):  
June B. Nasrallah ◽  
Mikhail E. Nasrallah
Keyword(s):  
Current Knowledge ◽  
Specific Interaction ◽  
The Self ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Pollen Coat ◽  
Allele Specific ◽  
Recognition And Response

SRK (S-locus receptor kinase) is the receptor that allows stigma epidermal cells to discriminate between genetically related (‘self’) and genetically unrelated (‘non-self’) pollen in the self-incompatibility response of the Brassicaceae. SRK and its ligand, the pollen coat-localized SCR (S-locus cysteine-rich protein), are highly polymorphic, and their allele-specific interaction explains specificity in the self-incompatibility response. The present article reviews current knowledge of the role of SRK in the recognition and response phases of self-incompatibility, and highlights the new insights provided by analysis of a transgenic self-incompatible Arabidopsis thaliana model.

scholarly journals Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2186
Author(s):  
Zhiquan Liu ◽  
Bing Li ◽  
Yong Yang ◽  
Changbin Gao ◽  
Bin Yi ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Locus ◽  
Recognition System ◽  
Inbred Lines ◽  
The Self ◽  
Self Incompatibility ◽  
S Locus ◽  
S Haplotype ◽  
Self Compatibility

Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus.

scholarly journals The Self-Incompatibility Phenotype in Brassica Is Altered by the Transformation of a Mutant S Locus Receptor Kinase

1998 ◽  
Vol 10 (2) ◽  
pp. 209
Author(s):  
Richard J. Stahl ◽  
Mary Anne Arnoldo ◽  
Tracy L. Glavin ◽  
Daphne R. Goring ◽  
Steven J. Rothstein
Keyword(s):  
The Self ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus

scholarly journals Genetic and Molecular Characterization of a Self-Compatible Brassica rapa Line Possessing a New Class II S Haplotype

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2815
Author(s):  
Bing Li ◽  
Xueli Zhang ◽  
Zhiquan Liu ◽  
Lulin Wang ◽  
Liping Song ◽  
...  
Keyword(s):  
Amino Acid ◽  
De Novo ◽  
Class Ii ◽  
Transcriptional Level ◽  
Self Incompatibility ◽  
S Locus ◽  
Multiple Sequence ◽  
Pak Choi ◽  
New Class ◽  
S Haplotype

Most flowering plants have evolved a self-incompatibility (SI) system to maintain genetic diversity by preventing self-pollination. The Brassica species possesses sporophytic self-incompatibility (SSI), which is controlled by the pollen- and stigma-determinant factors SP11/SCR and SRK. However, the mysterious molecular mechanism of SI remains largely unknown. Here, a new class II S haplotype, named BrS-325, was identified in a pak choi line ‘325’, which was responsible for the completely self-compatible phenotype. To obtain the entire S locus sequences, a complete pak choi genome was gained through Nanopore sequencing and de novo assembly, which provided a good reference genome for breeding and molecular research in B. rapa. S locus comparative analysis showed that the closest relatives to BrS-325 was BrS-60, and high sequence polymorphism existed in the S locus. Meanwhile, two duplicated SRKs (BrSRK-325a and BrSRK-325b) were distributed in the BrS-325 locus with opposite transcription directions. BrSRK-325b and BrSCR-325 were expressed normally at the transcriptional level. The multiple sequence alignment of SCRs and SRKs in class II S haplotypes showed that a number of amino acid variations were present in the contact regions (CR II and CR III) of BrSCR-325 and the hypervariable regions (HV I and HV II) of BrSRK-325s, which may influence the binding and interaction between the ligand and the receptor. Thus, these results suggested that amino acid variations in contact sites may lead to the SI destruction of a new class II S haplotype BrS-325 in B. rapa. The complete SC phenotype of ‘325’ showed the potential for practical breeding application value in B. rapa.

scholarly journals Genetic evidence for the requirement of the Brassica S-locus receptor kinase gene in the self-incompatibility response

1994 ◽  
Vol 5 (3) ◽  
pp. 373-384 ◽  
Author(s):  
June B. Nasrallah ◽  
Sabine J. Rundle ◽  
Mikhail E. Nasrallah
Keyword(s):  
Genetic Evidence ◽  
The Self ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Kinase Gene
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