Faculty Opinions recommendation of In vitro bioconversion of polyphenols from black tea and red wine/grape juice by human intestinal microbiota displays strong interindividual variability.

Studying potential dietary exposure to antimicrobial drug residues via meat and dairy products is essential to ensure human health and consumer safety. When studying how antimicrobial residues in food impact the development of antimicrobial drug resistance and disrupt normal bacteria community structure in the intestine, there are diverse methodological challenges to overcome. In this study, traditional cultures and molecular analysis techniques were used to determine the effects of tetracycline at chronic subinhibitory exposure levels on human intestinal microbiota using an in vitro continuous flow bioreactor. Six bioreactor culture vessels containing human fecal suspensions were maintained at 37 °C for 7 days. After a steady state was achieved, the suspensions were dosed with 0, 0.015, 0.15, 1.5, 15, or 150 µg/mL tetracycline, respectively. Exposure to 150 µg/mL tetracycline resulted in a decrease of total anaerobic bacteria from 1.9 × 107 ± 0.3 × 107 down to 2 × 106 ± 0.8 × 106 CFU/mL. Dose-dependent effects of tetracycline were noted for perturbations of tetB and tetD gene expression and changes in acetate and propionate concentrations. Although no-observed-adverse-effect concentrations differed, depending on the traditional cultures and the molecular analysis techniques used, this in vitro continuous flow bioreactor study contributes to the knowledge base regarding the impact of chronic exposure of tetracycline on human intestinal microbiota.

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The Modulatory Effect of Anthocyanins from Purple Sweet Potato on Human Intestinal Microbiota in Vitro

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.6b00586 ◽

2016 ◽

Vol 64 (12) ◽

pp. 2582-2590 ◽

Cited By ~ 49

Author(s):

Xin Zhang ◽

Yang Yang ◽

Zufang Wu ◽

Peifang Weng

Keyword(s):

Sweet Potato ◽

Intestinal Microbiota ◽

Human Intestinal Microbiota ◽

Purple Sweet Potato

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Fermentation in vitro of EGCG, GCG and EGCG3"Me isolated from Oolong tea by human intestinal microbiota

Food Research International ◽

10.1016/j.foodres.2013.10.005 ◽

2013 ◽

Vol 54 (2) ◽

pp. 1589-1595 ◽

Cited By ~ 57

Author(s):

Xin Zhang ◽

Xiuling Zhu ◽

Yongkang Sun ◽

Bing Hu ◽

Yi Sun ◽

...

Keyword(s):

Intestinal Microbiota ◽

Oolong Tea ◽

Human Intestinal Microbiota

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Salivary Proteins Interact with Dietary Constituents to Modulate Tooth Staining

Journal of Dental Research ◽

10.1177/154405910508400113 ◽

2005 ◽

Vol 84 (1) ◽

pp. 73-78 ◽

Cited By ~ 27

Author(s):

G.B. Proctor ◽

R. Pramanik ◽

G.H. Carpenter ◽

G.D. Rees

Keyword(s):

Red Wine ◽

Black Tea ◽

Salivary Protein ◽

Salivary Proteins ◽

Parotid Saliva ◽

Grape Skin ◽

Dietary Components ◽

Black Tea Polyphenols ◽

Proline Rich Protein

Dietary components rich in polyphenols—for example, tea and red wine—are thought to cause tooth staining. In the present study, hydroxyapatite was used as a model of enamel for study of the influence of salivary proteins on the binding of different polyphenols to hydroxyapatite in vitro. Neither salivary protein pellicles nor salivary proteins in solution significantly altered the binding of the small polyphenol epigallocatechin to hydroxyapatite. However, hydroxyapatite binding of anthocyanin, a small grape-skin-derived polyphenol, or the larger polyphenols of black tea was increased by the presence of salivary proteins, either as a pellicle or in solution. Proline-rich proteins were enriched from parotid saliva and found to increase binding of anthocyanin and black tea polyphenols to hydroxyapatite, while enriched histatins did not increase binding. It is concluded that some salivary proteins, including proline-rich protein, can mediate increased staining of enamel by red-wine- and black-tea-derived polyphenols.

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In vitro extraction and fermentation of polyphenols from grape seeds (Vitis vinifera) by human intestinal microbiota

Food & Function ◽

10.1039/c6fo00032k ◽

2016 ◽

Vol 7 (4) ◽

pp. 1959-1967 ◽

Cited By ~ 26

Author(s):

Li Zhou ◽

Wei Wang ◽

Jun Huang ◽

Yu Ding ◽

Zhouqiang Pan ◽

...

Keyword(s):

Vitis Vinifera ◽

Intestinal Microbiota ◽

Extraction Yield ◽

Liquid Extraction ◽

Pressurized Liquid Extraction ◽

Grape Seeds ◽

Total Polyphenols ◽

Human Intestinal Microbiota

The effects of several parameters on the extraction yield of total polyphenols from grape seeds by pressurized liquid extraction were investigated.

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Evaluation of the combined antibiotics exposure using the simulator of human intestinal microbial ecosystem (SHIME)

10.21203/rs.3.rs-46837/v1 ◽

2020 ◽

Author(s):

Lei Liu ◽

Hongmei Qi ◽

Pengcheng Suo ◽

Huai Lin ◽

Siyi Wang ◽

...

Keyword(s):

Health Risk ◽

Intestinal Microbiota ◽

Human Disease ◽

Alpha Diversity ◽

Intestinal Microbiome ◽

Limited Information ◽

Combination Effects ◽

Human Intestinal Microbiota ◽

Future Work

Abstract Background: Antibiotics are emerging toxic contaminant that have potential public health risk worldwide, which also would cause human intestinal microbial disorder and develop multiple human diseases. However, to date, the combination effects of antibiotics on human intestinal microbiota dysbiosis and related health risk are not fully understood. Moreover, there is limited information on using probiotics or synbiotics for restoration of intestinal microbiome affected by antibiotics. Therefore, this study evaluated the in vitro ability of combined effects of amoxicillin (Amx) and gentamycin (Gen), and the restoration effects of probiotics or synbiotics on ARGs as well as human disease-related pathways in the simulated human gut.Results: This study indicated that the combination exposure of Amx and Gen was confirmed to promote the increase of most ARGs and the disease-related pathways, which may be better restored by probiotics treatment. The results of the alpha diversity of the combined antibiotics exposure or the recovery microbial community showed no difference from the control. However, the beta diversity results indicated their differences, and the ascending colon sample recovered better under natural condition while the descending colon sample recovered better after probiotics treatment. Combination effects on the genetic level might attribute to microbiota shift, which were explained well by the phenomenon that Escherichia/Shigella was positively associated with the ARGs, and Klebsiella and Escherichia/Shigella were positively related to the human disease-related pathways.Conclusion: These results might be valuable to direct the future work and opened up new perspectives to address the direct effects of combine antibiotics on the intestinal microbiota and find a promising strategy to restore the antibiotics associated dysbiosis of gut microbiota.

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