Faculty Opinions recommendation of Contamination of nuclear fractions with plasma membrane lipid rafts.

2007 ◽  
Author(s):  
Michael Roth
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Membrane Lipid ◽  
Plasma Membrane Lipid

1P-0227 Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages

2003 ◽  
Vol 4 (2) ◽  
pp. 69 ◽  
Author(s):  
W. Jessup ◽  
K. Gaus ◽  
L. Kritharides ◽  
A. Boettcher ◽  
W. Drobnik ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Membrane Lipid ◽  
Apolipoprotein A ◽  
Plasma Membrane Lipid

scholarly journals Moderate Static Magnetic Field (6 mT)-Induced Lipid Rafts Rearrangement Increases Silver NPs Uptake in Human Lymphocytes

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1398
Author(s):  
Cristian Vergallo ◽  
Elisa Panzarini ◽  
Bernardetta Anna Tenuzzo ◽  
Stefania Mariano ◽  
Ada Maria Tata ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Lipid Raft ◽  
Human Lymphocytes ◽  
Peripheral Blood Lymphocytes ◽  
Membrane Lipid ◽  
Membrane Perturbation ◽  
Plasma Membrane Lipid ◽  
Silver Nps ◽  
Surface Ligand

One of the most relevant drawbacks in medicine is the ability of drugs and/or imaging agents to reach cells. Nanotechnology opened new horizons in drug delivery, and silver nanoparticles (AgNPs) represent a promising delivery vehicle for their adjustable size and shape, high-density surface ligand attachment, etc. AgNPs cellular uptake involves different endocytosis mechanisms, including lipid raft-mediated endocytosis. Since static magnetic fields (SMFs) exposure induces plasma membrane perturbation, including the rearrangement of lipid rafts, we investigated whether SMF could increase the amount of AgNPs able to pass the peripheral blood lymphocytes (PBLs) plasma membrane. To this purpose, the effect of 6-mT SMF exposure on the redistribution of two main lipid raft components (i.e., disialoganglioside GD3, cholesterol) and on AgNPs uptake efficiency was investigated. Results showed that 6 mT SMF: (i) induces a time-dependent GD3 and cholesterol redistribution in plasma membrane lipid rafts and modulates gene expression of ATP-binding cassette transporter A1 (ABCA1), (ii) increases reactive oxygen species (ROS) production and lipid peroxidation, (iii) does not induce cell death and (iv) induces lipid rafts rearrangement, that, in turn, favors the uptake of AgNPs. Thus, it derives that SMF exposure could be exploited to enhance the internalization of NPs-loaded therapeutic or diagnostic molecules.

scholarly journals Membrane lipid rafts are disturbed in the response of rat skeletal muscle to short-term disuse

2017 ◽  
Vol 312 (5) ◽  
pp. C627-C637 ◽  
Author(s):  
Alexey M. Petrov ◽  
Violetta V. Kravtsova ◽  
Vladimir V. Matchkov ◽  
Alexander N. Vasiliev ◽  
Andrey L. Zefirov ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Lipid Rafts ◽  
Lipid Raft ◽  
Motor Nerve ◽  
Membrane Lipid ◽  
Hindlimb Suspension ◽  
Cholera Toxin B Subunit ◽  
Plasma Membrane Lipid ◽  
Intracellular Ca

Marked loss of skeletal muscle mass occurs under various conditions of disuse, but the molecular and cellular mechanisms leading to atrophy are not completely understood. We investigate early molecular events that might play a role in skeletal muscle remodeling during mechanical unloading (disuse). The effects of acute (6–12 h) hindlimb suspension on the soleus muscles from adult rats were examined. The integrity of plasma membrane lipid rafts was tested utilizing cholera toxin B subunit or fluorescent sterols. In addition, resting intracellular Ca2+ level was analyzed. Acute disuse disturbed the plasma membrane lipid-ordered phase throughout the sarcolemma and was more pronounced in junctional membrane regions. Ouabain (1 µM), which specifically inhibits the Na-K-ATPase α2 isozyme in rodent skeletal muscles, produced similar lipid raft changes in control muscles but was ineffective in suspended muscles, which showed an initial loss of α2 Na-K-ATPase activity. Lipid rafts were able to recover with cholesterol supplementation, suggesting that disturbance results from cholesterol loss. Repetitive nerve stimulation also restores lipid rafts, specifically in the junctional sarcolemma region. Disuse locally lowered the resting intracellular Ca2+ concentration only near the neuromuscular junction of muscle fibers. Our results provide evidence to suggest that the ordering of lipid rafts strongly depends on motor nerve input and may involve interactions with the α2 Na-K-ATPase. Lipid raft disturbance, accompanied by intracellular Ca2+ dysregulation, is among the earliest remodeling events induced by skeletal muscle disuse.

scholarly journals Analysis of Detergent-Resistant Membranes in Arabidopsis. Evidence for Plasma Membrane Lipid Rafts

2004 ◽  
Vol 137 (1) ◽  
pp. 104-116 ◽  
Author(s):  
Georg H.H. Borner ◽  
D. Janine Sherrier ◽  
Thilo Weimar ◽  
Louise V. Michaelson ◽  
Nathan D. Hawkins ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Membrane Lipid ◽  
Plasma Membrane Lipid ◽  
Detergent Resistant Membranes

scholarly journals Cell-To-Cell Variability in Plasma Membrane Lipid Rafts

2013 ◽  
Vol 104 (2) ◽  
pp. 191a-192a
Author(s):  
Roland Schwarzer ◽  
Silvia Scolari ◽  
Reismann David ◽  
Rolf Schwarzer ◽  
Andreas Herrmann
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Membrane Lipid ◽  
Plasma Membrane Lipid ◽  
Cell To Cell Variability

Interaction of phosphatidylinositolglycan(-peptides) with plasma membrane lipid rafts triggers insulin-mimetic signaling in rat adipocytes

2002 ◽  
Vol 408 (1) ◽  
pp. 7-16 ◽  
Author(s):  
Günter Müller ◽  
Christian Jung ◽  
Wendelin Frick ◽  
Wolfhard Bandlow ◽  
Werner Kramer
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Membrane Lipid ◽  
Rat Adipocytes ◽  
Insulin Mimetic ◽  
Plasma Membrane Lipid

Interaction of phosphoinositolglycan(-peptides) with plasma membrane lipid rafts of rat adipocytes

2002 ◽  
Vol 408 (1) ◽  
pp. 17-32 ◽  
Author(s):  
Günter Müller ◽  
Nils Hanekop ◽  
Werner Kramer ◽  
Wolfhard Bandlow ◽  
Wendelin Frick
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Membrane Lipid ◽  
Rat Adipocytes ◽  
Plasma Membrane Lipid

Contamination of nuclear fractions with plasma membrane lipid rafts

PROTEOMICS ◽  
2007 ◽  
Vol 7 (7) ◽  
pp. 1059-1064 ◽  
Author(s):  
Yee-How Say ◽  
Nigel M. Hooper
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Membrane Lipid ◽  
Plasma Membrane Lipid

scholarly journals The Cytosolic Domains of Ca2+-sensitive Adenylyl Cyclases Dictate Their Targeting to Plasma Membrane Lipid Rafts

2004 ◽  
Vol 280 (8) ◽  
pp. 6380-6391 ◽  
Author(s):  
Andrew J. Crossthwaite ◽  
Thomas Seebacher ◽  
Nanako Masada ◽  
Antonio Ciruela ◽  
Kim Dufraux ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lipid Rafts ◽  
Membrane Lipid ◽  
Adenylyl Cyclases ◽  
Plasma Membrane Lipid ◽  
Cytosolic Domains
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