Faculty Opinions recommendation of Induction of focal adhesions and motility in Drosophila S2 cells.

2014 ◽  
Author(s):  
Miguel Vicente-Manzanares
Keyword(s):  
Focal Adhesions ◽  
S2 Cells ◽  
Drosophila S2 Cells

scholarly journals Induction of focal adhesions and motility in Drosophila S2 cells

2014 ◽  
Vol 25 (24) ◽  
pp. 3861-3869 ◽  
Author(s):  
Susana A. Ribeiro ◽  
Michael V. D'Ambrosio ◽  
Ronald D. Vale
Keyword(s):  
Focal Adhesion ◽  
Mammalian Cells ◽  
Focal Adhesions ◽  
S2 Cells ◽  
Cell Interior ◽  
Drosophila S2 Cells ◽  
Cytoskeletal Dynamics ◽  
A Cell ◽  
P21 Activated Kinase ◽  
Dynamic Structures

Focal adhesions are dynamic structures that interact with the extracellular matrix on the cell exterior and actin filaments on the cell interior, enabling cells to adhere and crawl along surfaces. We describe a system for inducing the formation of focal adhesions in normally non–ECM-adherent, nonmotile Drosophila S2 cells. These focal adhesions contain the expected molecular markers such as talin, vinculin, and p130Cas, and they require talin for their formation. The S2 cells with induced focal adhesions also display a nonpolarized form of motility on vitronectin-coated substrates. Consistent with findings in mammalian cells, the degree of motility can be tuned by changing the stiffness of the substrate and was increased after the depletion of PAK3, a p21-activated kinase. A subset of nonmotile, nonpolarized cells also exhibited focal adhesions that rapidly assembled and disassembled around the cell perimeter. Such cooperative and dynamic fluctuations of focal adhesions were decreased by RNA interference (RNAi) depletion of myosin II and focal adhesion kinase, suggesting that this behavior requires force and focal adhesion maturation. These results demonstrate that S2 cells, a cell line that is well studied for cytoskeletal dynamics and readily amenable to protein manipulation by RNAi, can be used to study the assembly and dynamics of focal adhesions and mechanosensitive cell motility.

Suppression of  -N-acetylglucosaminidase in the N-glycosylation pathway for complex glycoprotein formation in Drosophila S2 cells

Glycobiology ◽  
2008 ◽  
Vol 19 (3) ◽  
pp. 301-308 ◽  
Author(s):  
Y. K. Kim ◽  
K. R. Kim ◽  
D. G. Kang ◽  
S. Y. Jang ◽  
Y. H. Kim ◽  
...  
Keyword(s):  
S2 Cells ◽  
Drosophila S2 Cells ◽  
Glycosylation Pathway

scholarly journals Dissection of a Hypoxia-induced, Nitric Oxide–mediated Signaling Cascade

2009 ◽  
Vol 20 (18) ◽  
pp. 4083-4090 ◽  
Author(s):  
Pascale F. Dijkers ◽  
Patrick H. O'Farrell
Keyword(s):  
Nitric Oxide ◽  
Signal Transduction ◽  
Signaling Cascade ◽  
S2 Cells ◽  
Signal Transduction System ◽  
Signaling Systems ◽  
Calcium Dependent ◽  
Drosophila S2 Cells ◽  
Oxide Synthase ◽  
Multiple Signaling

Befitting oxygen's key role in life's processes, hypoxia engages multiple signaling systems that evoke pervasive adaptations. Using surrogate genetics in a powerful biological model, we dissect a poorly understood hypoxia-sensing and signal transduction system. Hypoxia triggers NO-dependent accumulation of cyclic GMP and translocation of cytoplasmic GFP-Relish (an NFκB/Rel transcription factor) to the nucleus in Drosophila S2 cells. An enzyme capable of eliminating NO interrupted signaling specifically when it was targeted to the mitochondria, arguing for a mitochondrial NO signal. Long pretreatment with an inhibitor of nitric oxide synthase (NOS), L-NAME, blocked signaling. However, addition shortly before hypoxia was without effect, suggesting that signaling is supported by the prior action of NOS and is independent of NOS action during hypoxia. We implicated the glutathione adduct, GSNO, as a signaling mediator by showing that overexpression of the cytoplasmic enzyme catalyzing its destruction, GSNOR, blocks signaling, whereas knockdown of this activity caused reporter translocation in the absence of hypoxia. In downstream steps, cGMP accumulated, and calcium-dependent signaling was subsequently activated via cGMP-dependent channels. These findings reveal the use of unconventional steps in an NO pathway involved in sensing hypoxia and initiating signaling.

RNAi in Drosophila S2 Cells by siRNA Duplex or dsRNA Transfection

2019 ◽  
Vol 2019 (4) ◽  
pp. pdb.prot097485 ◽  
Author(s):  
Chengjian Li ◽  
Phillip D. Zamore
Keyword(s):  
S2 Cells ◽  
Sirna Duplex ◽  
Drosophila S2 Cells
Sign in / Sign up

Export Citation Format

Share Document