Suppression of  -N-acetylglucosaminidase in the N-glycosylation pathway for complex glycoprotein formation in Drosophila S2 cells

Glycobiology ◽  
2008 ◽  
Vol 19 (3) ◽  
pp. 301-308 ◽  
Author(s):  
Y. K. Kim ◽  
K. R. Kim ◽  
D. G. Kang ◽  
S. Y. Jang ◽  
Y. H. Kim ◽  
...  
Keyword(s):  
S2 Cells ◽  
Drosophila S2 Cells ◽  
Glycosylation Pathway

scholarly journals Dissection of a Hypoxia-induced, Nitric Oxide–mediated Signaling Cascade

2009 ◽  
Vol 20 (18) ◽  
pp. 4083-4090 ◽  
Author(s):  
Pascale F. Dijkers ◽  
Patrick H. O'Farrell
Keyword(s):  
Nitric Oxide ◽  
Signal Transduction ◽  
Signaling Cascade ◽  
S2 Cells ◽  
Signal Transduction System ◽  
Signaling Systems ◽  
Calcium Dependent ◽  
Drosophila S2 Cells ◽  
Oxide Synthase ◽  
Multiple Signaling

Befitting oxygen's key role in life's processes, hypoxia engages multiple signaling systems that evoke pervasive adaptations. Using surrogate genetics in a powerful biological model, we dissect a poorly understood hypoxia-sensing and signal transduction system. Hypoxia triggers NO-dependent accumulation of cyclic GMP and translocation of cytoplasmic GFP-Relish (an NFκB/Rel transcription factor) to the nucleus in Drosophila S2 cells. An enzyme capable of eliminating NO interrupted signaling specifically when it was targeted to the mitochondria, arguing for a mitochondrial NO signal. Long pretreatment with an inhibitor of nitric oxide synthase (NOS), L-NAME, blocked signaling. However, addition shortly before hypoxia was without effect, suggesting that signaling is supported by the prior action of NOS and is independent of NOS action during hypoxia. We implicated the glutathione adduct, GSNO, as a signaling mediator by showing that overexpression of the cytoplasmic enzyme catalyzing its destruction, GSNOR, blocks signaling, whereas knockdown of this activity caused reporter translocation in the absence of hypoxia. In downstream steps, cGMP accumulated, and calcium-dependent signaling was subsequently activated via cGMP-dependent channels. These findings reveal the use of unconventional steps in an NO pathway involved in sensing hypoxia and initiating signaling.

scholarly journals Evidence for a rapid cold hardening response in cultured Drosophila S2 cells

2019 ◽  
Vol 223 (2) ◽  
pp. jeb212613 ◽  
Author(s):  
Emily A. W. Nadeau ◽  
Nicholas M. Teets
Keyword(s):  
Cold Hardening ◽  
S2 Cells ◽  
Rapid Cold Hardening ◽  
Drosophila S2 Cells

RNAi in Drosophila S2 Cells by siRNA Duplex or dsRNA Transfection

2019 ◽  
Vol 2019 (4) ◽  
pp. pdb.prot097485 ◽  
Author(s):  
Chengjian Li ◽  
Phillip D. Zamore
Keyword(s):  
S2 Cells ◽  
Sirna Duplex ◽  
Drosophila S2 Cells
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