Faculty Opinions recommendation of Regulation of hypothalamic signaling by tuberoinfundibular peptide of 39 residues is critical for the response to cold: a novel peptidergic mechanism of thermoregulation.

2016 ◽  
Author(s):  
Shaun Morrison
Keyword(s):  
Tuberoinfundibular Peptide

scholarly journals Methods to Enhance Signal Using Isotopic In Situ Hybridization

2002 ◽  
Vol 50 (8) ◽  
pp. 1031-1037 ◽  
Author(s):  
Betty Ky ◽  
Paul J. Shughrue
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Cell Types ◽  
Oxytocin Receptor ◽  
Specific Cell ◽  
Rat Hypothalamus ◽  
Low Levels ◽  
Tuberoinfundibular Peptide ◽  
Cryostat Sections

Isotopic in situ hybridization (ISH) has been established as a uniquely powerful tool for the study of gene expression in specific cell types. This technique allows the visualization and quantification of gene expression and gene expression changes in cells. In our study of biological and molecular phenomena, we have increasingly encountered the need to detect small changes in gene expression as well as genes of low abundance, such as the oxytocin receptor (OTR) and the tuberoinfundibular peptide of 39 residues (Tip39). To increase the sensitivity of isotopic ISH for detection of rare mRNAs, we performed ISH on cryostat sections of rat hypothalamus and thalamus with 35S-labeled riboprobes and amplified the signal by hybridizing over 2 nights as well as labeling the probe with both [35S]-UTP and [35S]-ATP. These two methods of enhancement independently and in combination demonstrated a dramatic increase in signal, allowing the visualization of low levels of gene expression previously undetectable by conventional methods.

scholarly journals Identification and Characterization of the Murine and Human Gene Encoding the Tuberoinfundibular Peptide of 39 Residues

Endocrinology ◽  
2002 ◽  
Vol 143 (3) ◽  
pp. 1047-1057 ◽  
Author(s):  
Markus R. John ◽  
Maya Arai ◽  
David A. Rubin ◽  
Kenneth B. Jonsson ◽  
Harald Jüppner
Keyword(s):  
Human Gene ◽  
Gene Encoding ◽  
Tuberoinfundibular Peptide ◽  
Identification And Characterization

scholarly journals Transcript Expression of the Tuberoinfundibular Peptide (TIP)39/PTH2 Receptor System and Non-PTH1 Receptor-Mediated Tonic Effects of TIP39 and Other PTH2 Receptor Ligands in Renal Vessels

Endocrinology ◽  
2002 ◽  
Vol 143 (8) ◽  
pp. 3036-3043 ◽  
Author(s):  
Anne Eichinger ◽  
Nathalie Fiaschi-Taesch ◽  
Thierry Massfelder ◽  
Samuel Fritsch ◽  
Mariette Barthelmebs ◽  
...  
Keyword(s):  
Transcript Expression ◽  
Receptor Ligands ◽  
Receptor System ◽  
Renal Vessels ◽  
Tuberoinfundibular Peptide ◽  
Pth1 Receptor

scholarly journals Tuberoinfundibular peptide of 39 residues in the embryonic and early postnatal rat brain

2008 ◽  
Vol 36 (1) ◽  
pp. 59-68 ◽  
Author(s):  
Dávid Brenner ◽  
Attila G. Bagó ◽  
Katalin Gallatz ◽  
Miklós Palkovits ◽  
Ted Björn Usdin ◽  
...  
Keyword(s):  
Rat Brain ◽  
Tuberoinfundibular Peptide
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