Faculty Opinions recommendation of Real-time Raman spectroscopy for in vivo, online gastric cancer diagnosis during clinical endoscopic examination.

This study aims to assess the clinical utility of a rapid fiber-optic Raman spectroscopy technique developed for enhancingin vivodiagnosis of gastric precancer during endoscopic examination. We have developed a real-time fiber-optic Raman spectroscopy system capable of simultaneously acquiring both fingerprint (FP) (i.e., 800–1800 cm−1) and high-wavenumber (HW) (i.e., 2800–3600 cm−1) Raman spectra from gastric tissuein vivoat endoscopy. A total of 5792 high-qualityin vivoFP/HW Raman spectra (normal (n= 5160); dysplasia (n= 155), and adenocarcinoma (n= 477)) were acquired in real-time from 441 tissue sites (normal (n= 396); dysplasia (n= 11), and adenocarcinoma (n= 34)) of 191 gastric patients (normal (n= 172); dysplasia (n= 6), and adenocarcinoma (n= 13)) undergoing routine endoscopic examinations. Partial least squares discriminant analysis (PLS-DA) together with leave-one-patient-out cross validation (LOPCV) were implemented to develop robust spectral diagnostic models. The FP/HW Raman spectra differ significantly between normal, dysplasia and adenocarcinoma of the stomach, which can be attributed to changes in proteins, lipids, nucleic acids, and the bound water content. PLS-DA and LOPCV show that the fiber-optic FP/HW Raman spectroscopy provides diagnostic sensitivities of 96.0%, 81.8% and 88.2%, and specificities of 86.7%, 95.3% and 95.6%, respectively, for the classification of normal, dysplastic and cancerous gastric tissue, superior to either the FP or HW Raman techniques alone. Further dichotomous PLS-DA analysis yields a sensitivity of 90.9% (10/11) and specificity of 95.9% (380/396) for the detection of gastric dysplasia using FP/HW Raman spectroscopy, substantiating its clinical advantages over white light reflectance endoscopy (sensitivity: 90.9% (10/11), and specificity: 51.0% (202/396)). This work demonstrates that the fiber-optic FP/HW Raman spectroscopy technique has great promise for enhancingin vivodiagnosis of gastric precancer during routine endoscopic examination.

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In Vivo Bladder Cancer Diagnosis by High-Volume Raman Spectroscopy

Analytical Chemistry ◽

10.1021/ac100448p ◽

2010 ◽

Vol 82 (14) ◽

pp. 5993-5999 ◽

Cited By ~ 129

Author(s):

Ronald O. P. Draga ◽

Matthijs C. M. Grimbergen ◽

Peter L. M. Vijverberg ◽

Christiaan F. P. van Swol ◽

Trudy G. N. Jonges ◽

...

Keyword(s):

Raman Spectroscopy ◽

Bladder Cancer ◽

Cancer Diagnosis ◽

High Volume

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Cooperative Raman Spectroscopy for Real-Time In Vivo Nano-Biosensing

IEEE Transactions on NanoBioscience ◽

10.1109/tnb.2017.2749183 ◽

2017 ◽

Vol 16 (7) ◽

pp. 571-584 ◽

Cited By ~ 1

Author(s):

Hongzhi Guo ◽

Josep Miquel Jornet ◽

Qiaoqiang Gan ◽

Zhi Sun

Keyword(s):

Raman Spectroscopy ◽

Real Time

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Real-time In vivo Diagnosis of Nasopharyngeal Carcinoma Using Rapid Fiber-Optic Raman Spectroscopy

Theranostics ◽

10.7150/thno.16359 ◽

2017 ◽

Vol 7 (14) ◽

pp. 3517-3526 ◽

Cited By ~ 26

Author(s):

Kan Lin ◽

Wei Zheng ◽

Chwee Ming Lim ◽

Zhiwei Huang

Keyword(s):

Raman Spectroscopy ◽

Nasopharyngeal Carcinoma ◽

Real Time ◽

Fiber Optic ◽

In Vivo Diagnosis

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Ultrasound and Transcriptomics Identify a Differential Impact of Cisplatin and Histone Deacetylation on Tumor Structure and Microenvironment in a Patient-Derived In Vivo Model of Gastric Cancer

Pharmaceutics ◽

10.3390/pharmaceutics13091485 ◽

2021 ◽

Vol 13 (9) ◽

pp. 1485

Author(s):

Aina Venkatasamy ◽

Eric Guerin ◽

Anais Blanchet ◽

Christophe Orvain ◽

Véronique Devignot ◽

...

Keyword(s):

Gastric Cancer ◽

Tumor Microenvironment ◽

Real Time ◽

Histone Deacetylase Inhibitors ◽

Structural Changes ◽

Histone Deacetylation ◽

In Vivo Model ◽

Specific Cell ◽

The Impact

The reasons behind the poor efficacy of transition metal-based chemotherapies (e.g., cisplatin) or targeted therapies (e.g., histone deacetylase inhibitors, HDACi) on gastric cancer (GC) remain elusive and recent studies suggested that the tumor microenvironment could contribute to the resistance. Hence, our objective was to gain information on the impact of cisplatin and the pan-HDACi SAHA (suberanilohydroxamic acid) on the tumor substructure and microenvironment of GC, by establishing patient-derived xenografts of GC and a combination of ultrasound, immunohistochemistry, and transcriptomics to analyze. The tumors responded partially to SAHA and cisplatin. An ultrasound gave more accurate tumor measures than a caliper. Importantly, an ultrasound allowed a noninvasive real-time access to the tumor substructure, showing differences between cisplatin and SAHA. These differences were confirmed by immunohistochemistry and transcriptomic analyses of the tumor microenvironment, identifying specific cell type signatures and transcription factor activation. For instance, cisplatin induced an “epithelial cell like” signature while SAHA favored a “mesenchymal cell like” one. Altogether, an ultrasound allowed a precise follow-up of the tumor progression while enabling a noninvasive real-time access to the tumor substructure. Combined with transcriptomics, our results underline the different intra-tumoral structural changes caused by both drugs that impact differently on the tumor microenvironment.

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