Faculty Opinions recommendation of Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate.

2017 ◽  
Author(s):  
Ken Yokoyama
Keyword(s):  
Phase Plate

Characteristics of an Electrostatic Phase Plate

1972 ◽  
Vol 30 ◽  
pp. 618-619
Author(s):  
William Krakow ◽  
Benjamin Siegel
Keyword(s):  
Phase Contrast ◽  
Objective Lens ◽  
Phase Plate ◽  
Net Charge ◽  
Phase Plates ◽  
Beam Currents ◽  
Contrast Image ◽  
Bright Phase ◽  
Additional Phase Shift ◽  
Additional Phase

Unwin has used a metallized non-conducting thread in the back focal plane of the objective lens that stops out a portion of the unscattered beam, takes on a localized positive charge and thus produces an additional phase shift to give a different transfer function of the lens. Under the particular conditions Unwin used, the phase contrast image was shifted to bright phase contrast for optimum focus.We have investigated the characteristics of this type of electrostatic phase plate, both analytically and experimentally, as functions of the magnitude of charge and defocus. Phase plates have been constructed by using Wollaston wire to mount 0.25μ diameter platinum wires across apertures ranging from 50 to 200μ diameter and vapor depositing SiO and gold on the mounted wires to give them the desired charging characteristics. The net charge was varied by adjusting only the bias on the Wehnelt shield of the gun, and hence the beam currents and effective size of the source.

scholarly journals High-Accuracy Surface Topography Manufacturing for Continuous Phase Plates Using an Atmospheric Pressure Plasma Jet

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 683
Author(s):  
Huiliang Jin ◽  
Caixue Tang ◽  
Haibo Li ◽  
Yuanhang Zhang ◽  
Yaguo Li
Keyword(s):  
Surface Topography ◽  
Atmospheric Pressure ◽  
High Efficiency ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Continuous Phase ◽  
High Accuracy ◽  
Phase Plate ◽  
Atmospheric Pressure Plasma Jet ◽  
Pressure Plasma

The continuous phase plate (CPP) is the vital diffractive optical element involved in laser beam shaping and smoothing in high-power laser systems. The high gradients, small spatial periods, and complex features make it difficult to achieve high accuracy when manufacturing such systems. A high-accuracy and high-efficiency surface topography manufacturing method for CPP is presented in this paper. The atmospheric pressure plasma jet (APPJ) system is presented and the removal characteristics are studied to obtain the optimal processing parameters. An optimized iterative algorithm based on the dwell point matrix and a fast Fourier transform (FFT) is proposed to improve the accuracy and efficiency in the dwell time calculation process. A 120 mm × 120 mm CPP surface topography with a 1326.2 nm peak-to-valley (PV) value is fabricated with four iteration steps after approximately 1.6 h of plasma processing. The residual figure error between the prescribed surface topography and plasma-processed surface topography is 28.08 nm root mean square (RMS). The far-field distribution characteristic of the plasma-fabricated surface is analyzed, for which the energy radius deviation is 11 μm at 90% encircled energy. The experimental results demonstrates the potential of the APPJ approach for the manufacturing of complex surface topographies.

Colorful holographic imaging reconstruction based on one thin phase plate

2014 ◽  
Author(s):  
Jing Zhu ◽  
Qiang Song ◽  
Jian Wang ◽  
Weirui Yue ◽  
Fang Zhang ◽  
...  
Keyword(s):  
Phase Plate ◽  
Holographic Imaging ◽  
Imaging Reconstruction

scholarly journals Phase Plate Enhanced Contrast Reveals Structure of Follicular Melanin Granules

2011 ◽  
Vol 17 (S2) ◽  
pp. 984-985
Author(s):  
G Camacho-Bragado ◽  
H Qian ◽  
F Dixon ◽  
H Bryant ◽  
M Malac
Keyword(s):  
Phase Plate

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

Fabrication of optical multilayer for two-color phase plate in super-resolution microscope

2014 ◽  
Vol 85 (7) ◽  
pp. 073701
Author(s):  
Yoshinori Iketaki ◽  
Katsuichi Kitagawa ◽  
Kohjiro Hidaka ◽  
Naoki Kato ◽  
Akira Hirabayashi ◽  
...  
Keyword(s):  
Super Resolution ◽  
Phase Plate
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