Faculty Opinions recommendation of Spatiotemporal structure of cell fate decisions in murine neural crest.

2019 ◽  
Author(s):  
Eloisa Herrera ◽  
Aida Giner de Gracia
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Spatiotemporal Structure ◽  
Cell Fate Decisions

scholarly journals Cell fate decisions during neural crest ontogeny

2017 ◽  
Vol 61 (3-4-5) ◽  
pp. 195-203 ◽  
Author(s):  
Chaya Kalcheim ◽  
Deepak Kumar
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Cell Fate Decisions

scholarly journals Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Achim Werner ◽  
Regina Baur ◽  
Nia Teerikorpi ◽  
Deniz U Kaya ◽  
Michael Rape
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Posttranslational Modifications ◽  
E3 Ligase ◽  
Cell Fate Decisions ◽  
Regulatory Circuit ◽  
Dependent Cell ◽  
Slow Rise ◽  
Regulated Gene Expression ◽  
Powerful Mechanism

Metazoan development depends on tightly regulated gene expression programs that instruct progenitor cells to adopt specialized fates. Recent work found that posttranslational modifications, such as monoubiquitylation, can determine cell fate also independently of effects on transcription, yet how monoubiquitylation is implemented during development is poorly understood. Here, we have identified a regulatory circuit that controls monoubiquitylation-dependent neural crest specification by the E3 ligase CUL3 and its substrate adaptor KBTBD8. We found that CUL3KBTBD8 monoubiquitylates its essential targets only after these have been phosphorylated in multiple motifs by CK2, a kinase whose levels gradually increase during embryogenesis. Its dependency on multisite phosphorylation allows CUL3KBTBD8 to convert the slow rise in embryonic CK2 into decisive recognition of ubiquitylation substrates, which in turn is essential for neural crest specification. We conclude that multisite dependency of an E3 ligase provides a powerful mechanism for switch-like cell fate transitions controlled by monoubiquitylation.

scholarly journals Pre-Border Gene Foxb1 Regulates the Differentiation Timing and Autonomic Neuronal Potential of Human Neural Crest Cells

2019 ◽  
Author(s):  
Alan W. Leung ◽  
Francesc López-Giráldez ◽  
Cayla Broton ◽  
Kaixuan Lin ◽  
Maneeshi S. Prasad ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Stem Cell Differentiation ◽  
Human Pluripotent Stem Cell ◽  
Cell Fate Decisions ◽  
Pluripotency Factors ◽  
Forkhead Box ◽  
Insight Into

SUMMARYWhat are the factors that are induced during the transitory phases from pluripotent stem cells to lineage specified cells, how are they regulated, and what are their functional contributions are fundamental questions for basic developmental biology and clinical research. Here, we uncover a set of pre-border (pB) gene candidates, including forkhead box B1 (FOXB1), induced during human neural crest (NC) cell development. We characterize their associated enhancers that are bound by pluripotency factors and rapidly activated by β-catenin-mediated signaling during differentiation. Surprisingly, the endogenous transient expression of FOXB1 directly regulates multiple early NC and neural progenitor loci including PAX7, MSX2, SOX1, and ASCL1, controls the timing of NC fate acquisition, and differentially activates autonomic neurogenic versus mesenchymal fates in mature NC cells. Our findings provide further insight into the concept of the less characterized pB state and clearly establishes FOXB1 as a key regulator in early cell fate decisions during human pluripotent stem cell differentiation.

scholarly journals Twist1 Controls a Cell-Specification Switch Governing Cell Fate Decisions within the Cardiac Neural Crest

PLoS Genetics ◽  
2013 ◽  
Vol 9 (3) ◽  
pp. e1003405 ◽  
Author(s):  
Joshua W. Vincentz ◽  
Beth A. Firulli ◽  
Andrea Lin ◽  
Douglas B. Spicer ◽  
Marthe J. Howard ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Cell Specification ◽  
Cell Fate Decisions ◽  
Cardiac Neural Crest ◽  
A Cell

scholarly journals Spatiotemporal structure of cell fate decisions in murine neural crest

Science ◽  
2019 ◽  
Vol 364 (6444) ◽  
pp. eaas9536 ◽  
Author(s):  
Ruslan Soldatov ◽  
Marketa Kaucka ◽  
Maria Eleni Kastriti ◽  
Julian Petersen ◽  
Tatiana Chontorotzea ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Single Cell Analysis ◽  
Neural Crest Cells ◽  
Cell Types ◽  
Phenotypic Traits ◽  
Cell Fate Decisions ◽  
Numerous Cell ◽  
Trunk Neural Crest ◽  
Cranial Neural Crest Cells

Neural crest cells are embryonic progenitors that generate numerous cell types in vertebrates. With single-cell analysis, we show that mouse trunk neural crest cells become biased toward neuronal lineages when they delaminate from the neural tube, whereas cranial neural crest cells acquire ectomesenchyme potential dependent on activation of the transcription factor Twist1. The choices that neural crest cells make to become sensory, glial, autonomic, or mesenchymal cells can be formalized as a series of sequential binary decisions. Each branch of the decision tree involves initial coactivation of bipotential properties followed by gradual shifts toward commitment. Competing fate programs are coactivated before cells acquire fate-specific phenotypic traits. Determination of a specific fate is achieved by increased synchronization of relevant programs and concurrent repression of competing fate programs.

scholarly journals Epigenetic dynamics shaping melanophore and iridophore cell fate in zebrafish

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hyo Sik Jang ◽  
Yujie Chen ◽  
Jiaxin Ge ◽  
Alicia N. Wilkening ◽  
Yiran Hou ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Differentiation ◽  
Neural Crest ◽  
Cell Fate ◽  
Pigment Cell ◽  
Chromatin Accessibility ◽  
Pigment Cells ◽  
Epigenetic Landscape ◽  
Cell Fate Decisions ◽  
Neural Crest Differentiation

Abstract Background Zebrafish pigment cell differentiation provides an attractive model for studying cell fate progression as a neural crest progenitor engenders diverse cell types, including two morphologically distinct pigment cells: black melanophores and reflective iridophores. Nontrivial classical genetic and transcriptomic approaches have revealed essential molecular mechanisms and gene regulatory circuits that drive neural crest-derived cell fate decisions. However, how the epigenetic landscape contributes to pigment cell differentiation, especially in the context of iridophore cell fate, is poorly understood. Results We chart the global changes in the epigenetic landscape, including DNA methylation and chromatin accessibility, during neural crest differentiation into melanophores and iridophores to identify epigenetic determinants shaping cell type-specific gene expression. Motif enrichment in the epigenetically dynamic regions reveals putative transcription factors that might be responsible for driving pigment cell identity. Through this effort, in the relatively uncharacterized iridophores, we validate alx4a as a necessary and sufficient transcription factor for iridophore differentiation and present evidence on alx4a’s potential regulatory role in guanine synthesis pathway. Conclusions Pigment cell fate is marked by substantial DNA demethylation events coupled with dynamic chromatin accessibility to potentiate gene regulation through cis-regulatory control. Here, we provide a multi-omic resource for neural crest differentiation into melanophores and iridophores. This work led to the discovery and validation of iridophore-specific alx4a transcription factor.

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