Faculty Opinions recommendation of Atomic Resolution Map of Hierarchical Self-Assembly for an Amyloidogenic Protein Probed through Thermal 15N-R2 Correlation Matrices.

Atomic resolution map of the soluble amyloid beta assembly (Aβn) “toxic surfaces” that facilitate the early pathogenic events in Alzheimer's disease (AD).

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Tubulin Lattice in Cilia is in a Stressed Form Regulated by Microtubule Inner Proteins

10.1101/596478 ◽

2019 ◽

Cited By ~ 3

Author(s):

Muneyoshi Ichikawa ◽

Ahmad Abdelzaher Khalifa ◽

Kaustuv Basu ◽

Daniel Dai ◽

Mohammad Amin Faghfor Maghrebi ◽

...

Keyword(s):

Lattice Structure ◽

Atomic Resolution ◽

High Frequencies ◽

Lateral Angle ◽

A Cell ◽

Longitudinal Compaction ◽

Resolution Map

AbstractCilia, the hair-like protrusions that beat at high frequencies to propel a cell or move fluid around the cell, are composed of radially bundled doublet microtubules. The doublet microtubule is composed of a 13-protofilament A-tubule, a partial 10-protofilament B-tubule and microtubule inner proteins (MIPs) inside the tubulin lattice. In this study, we present the near-atomic resolution map of theTetrahymenadoublet microtubules. The map demonstrates that the network of microtubule inner proteins is weaving into the tubulin lattice, forming an inner sheath of proteins. In addition, we also obtain the tubulin lattice structure with missing MIPs by Sarkosyl treatment. In this structure, the tubulin lattice showed significant longitudinal compaction and lateral angle changes between protofilaments. These results are evidence that the binding of MIPs directly affects and stabilizes the tubulin lattice. It is also suggested that the doublet microtubule is an intrinsically stressed filament and this stress could be exploited in the regulation of ciliary waveforms.

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Faculty Opinions recommendation of Atomic resolution map of the soluble amyloid beta assembly toxic surfaces.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.735890022.793560919 ◽

2019 ◽

Author(s):

Ayyalusamy Ramamoorthy

Keyword(s):

Amyloid Beta ◽

Atomic Resolution ◽

Resolution Map

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Harnessing complexity in molecular self-assembly using computer simulations

Physical Chemistry Chemical Physics ◽

10.1039/c7cp06181a ◽

2018 ◽

Vol 20 (10) ◽

pp. 6767-6776 ◽

Cited By ~ 4

Author(s):

Xiangze Zeng ◽

Lizhe Zhu ◽

Xiaoyan Zheng ◽

Marco Cecchini ◽

Xuhui Huang

Keyword(s):

Computer Simulations ◽

Self Assembly ◽

Atomic Resolution ◽

Computational Studies ◽

Powerful Strategy

Computer simulations offer a powerful strategy to explore self-assembly with atomic resolution. Here, we review recent computational studies focusing on both thermodynamic and kinetic aspects.

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