UJI POLIMORPIK DAN HETEROZIGOSITAS PADA PROGENI F4 KEDELAI (Glycine max (L.) Merril) TAHAN SALIN DENGAN MENGGUNAKAN MARKA SSR (Simple Sequence Repeats)

SSR marker has some merits such as quickness, simplicity, rich polymorphism and stability, thus being widely applied in genetic diversity analysis, molecular map construction and gene mapping. the purpose of this study was to determine polymorpic test and heterozygosity in F4 soybean (Glycine max (L.) Merril) progeni saline resistant characters using SSR (Simple Sequence Repeats) markers. This research was conducted in Biomolecular Laboratory, Socfindo Seed Production Laboratory (SSPL), Kebun Bangun Bandar Village Martebing District Dolok Masihul Regency Serdang Bedagai on December-May 2017. The number of samples were used 44. The five SSR primers (QS080465, QS1101, QS1112, QS100011, and Sat_091) used were specific primers, with a band pattern that was clearly visible around one or two bands. The percentage of polymorphic primers (PLP) of these three populations is high, all populations with a PLP of 100% of the saline resistant character. The effective allele number (Ne) of 7,160 for the progeny population is lower than the number of observed alleles (Na) of 10,000 which means that many progeny individuals are homozygous. The expected heterozygosity (He) value of 0.854 in the progeny population was higher than the observed heterozygosity (Ho) value of 0.027. The overall average observed heterozygosity (Ho) was 0.009 lower than the overall expected heterozygosity (He) of 0.61. This means that each character has a low heterozygosity.Keywords: Progeny F4, soybean, SSR, saline resistant, polymorphic, heterozygosity

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Keragaman Genetik 27 Aksesi Kedelai (Glycine Max L. Merr.) Introduksi Subtropis berdasarkan Marka SSR

Vegetalika ◽

10.22146/veg.58418 ◽

2021 ◽

Vol 10 (1) ◽

pp. 1

Author(s):

Puji Lestari ◽

Rizki Eka Putri ◽

Innaka Ageng Rineksane ◽

Etty Handayani ◽

Kristianto Nugroho ◽

...

Keyword(s):

Glycine Max ◽

Information Content ◽

Simple Sequence Repeats ◽

Polymorphism Information Content ◽

Glycine Max L ◽

Simple Sequence

Karakterisasi aksesi kedelai (Glycine max L. Merr.) dalam koleksi perlu dilakukan berdasarkan marka molekuler untuk mendukung karakter morfologi. Penelitian ini bertujuan untuk menganalisis keragaman genetik aksesi kedelai yang diintroduksi dari daerah subtropis menggunakan marka simple sequence repeats (SSR) yang didukung melalui informasi karakter morfologi. Hasil penelitian menunjukkan bahwa sebanyak 15 SSR berhasil dideteksi polimorfismenya pada 27 aksesi kedelai dari daerah subtropis dengan total 158 alel berukuran antara 100-368 bp dengan kisaran jumlah 4-18 alel per lokus. Rata-rata polymorphism information content (PIC) ditemukan sebesar 0,92 dengan nilai tertinggi 0,96 (SATT463, SATT249, SATT063) dan nilai terendah 0,87 (SATT038). Semua marka SSR memiliki nilai PIC >0,8 yang mengindikasikan sebagai marka sangat informatif, artinya mampu mendiferensiasi antar aksesi dan dapat diaplikasikan dalam mendeteksi keragaman genetik plasma nutfah kedelai lainnya. Keragaman genetik aksesi kedelai terebut sangat tinggi seperti yang direfleksikan oleh rata-rata indeks diversitas gen sebesar 0,93. Analisis klaster dengan marka SSR berhasil membagi 27 aksesi kedelai menjadi dua kelompok utama yang sebagian besar dalam kelompok I (26 aksesi) dan kelompok II khusus aksesi D76-8070. Karakterisasi molekuler dengan SSR tersebut mendukung keragaman yang tinggi karakter morfologi yang memisahkan total aksesi menjadi empat kuadran. Informasi keragaman genetik berdasarkan marka S yang didukung karakter morfologi tersebut dapat menjadi dasar awal seleksi tetua persilangan aksesi dari daerah subtropis untuk pengembangan varietas baru melalui pemuliaan kedelai di iklim tropis Indonesia.

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(C-A) and (G-A) anchored simple sequence repeats (ASSRs) generated polymorphism in soybean, Glycine max (L.) Merr.

Theoretical and Applied Genetics ◽

10.1007/s001220050843 ◽

1998 ◽

Vol 96 (8) ◽

pp. 1086-1096 ◽

Cited By ~ 10

Author(s):

G. Wang ◽

R. Mahalingam ◽

H. T. Knap

Keyword(s):

Glycine Max ◽

Simple Sequence Repeats ◽

Glycine Max L ◽

Simple Sequence

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Development of simple sequence repeat markers in rye (Secale cereale L.)

Genome ◽

10.1139/g99-052 ◽

1999 ◽

Vol 42 (5) ◽

pp. 964-972 ◽

Cited By ~ 89

Author(s):

B Saal ◽

Günter Wricke

Keyword(s):

Ssr Markers ◽

Secale Cereale ◽

Simple Sequence Repeats ◽

Rflp Markers ◽

Dinucleotide Repeats ◽

Genomic Libraries ◽

Allele Number ◽

Expected Heterozygosity ◽

Secale Cereale L ◽

Simple Sequence

Simple sequence repeats (SSRs), also referred to as microsatellites, represent a PCR-based marker system that has been described in mammalian and plant genomes in recent years. In self-pollinating crop plants they have been shown to be superior to other DNA markers with respect to their level of polymorphism. The technical advantages compared with RFLP markers should also facilitate marker analysis in outcrossing crops like rye. In order to determine the usefulness of SSR markers in rye genetics and breeding, several genomic libraries were screened for (CT/GA)n and (GT/CA)n dinucleotide repeats. It was estimated that these motifs occur at a frequency of one per 268-519 kb. Seventy four out of 182 positive clones were sequenced, and the majority (56.8%) revealed perfect repeats, predominantly of the type (GT/CA)n (61.9%). Fifty seven primer pairs were designed and 27 (47.4%) resulted in specific SSR markers, of which 20 were genetically mapped or assigned to chromosomes or chromosome arms, respectively. The level of polymorphism of four SSR and three RFLP markers was assessed in two open-pollinated rye cultivars. On average, the SSR markers showed larger values of expected heterozygosity (0.62 vs. 0.43) and allele number (5.9 vs. 3.4) than RFLP markers in both cultivars.Key words: simple sequence repeats, microsatellites, mapping, rye, Secale cereale.

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Genetic Diversity of Vitis davidii Accessions Revealed Using Microsatellite and Sequence-related Amplified Polymorphism Markers

HortScience ◽

10.21273/hortsci12506-17 ◽

2018 ◽

Vol 53 (3) ◽

pp. 283-287

Author(s):

Xiu Cai Fan ◽

Hai Sheng Sun ◽

Ying Zhang ◽

Jian Fu Jiang ◽

Min Li ◽

...

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Genetic Similarity ◽

Similarity Coefficient ◽

Srap Markers ◽

Average Percentage ◽

Allele Number ◽

Ssr Primers ◽

Vitis Davidii ◽

Simple Sequence

In this study, simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) markers were used to analyze the genetic diversity of 48 wild Vitis davidii accessions. A total of 78 distinct alleles were amplified by 11 SSR primers, and the average allele number was 8.8. The average observed heterozygosity (Ho) and expected heterozygosity (He) values were 0.785 and 0.814, respectively. The effective allele numbers ranged from 3.92 to 9.61. The average polymorphism information content (PIC) was 0.798. Twelve of 169 SRAP primer combinations were selected for SRAP analysis. A total of 188 bands were produced, and the average was 15.7 bands per primer combination; the average percentage of polymorphic bands was 84.0%. The average PIC was 0.76. The results of the clustering analysis based on SSR markers showed that the 48 wild V. davidii accessions could be classified into five main clusters and had a genetic similarity coefficient level of 0.68. The dendrogram obtained from the SRAP data showed that 48 wild V. davidii accessions could be classified into five main clusters and had a genetic similarity coefficient of 0.72. SSR and SRAP markers differentiated all accessions studied including those with a similar pedigree. We speculated on the origin of Ciputao 0941♀, Ciputao 0940♂, and Fu’an-ci-01 using SSR markers and used both SSR and SRAP markers to resolve homonymy. The result will be valuable for further management and protection of V. davidii germplasm resources.

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