scholarly journals Dynamique spatio-temporelle de la pourriture brune des cabosses de cacaoyer due à phytophthora sp. dans le département d’Aboisso (Cote d’Ivoire)

2020 ◽  
Vol 145 ◽  
pp. 14842-14852
Author(s):  
POHE Jean ◽  
MIAN Jacques Dutron ◽  
OKOU Staelle Florence Famisso

RESUME Objectif : Cette étude a été menée pour évaluer le niveau de la pourriture brune, de suivre son évolution dans les plantations, au niveau des différentes parties du cacaoyer et de préciser l’emplacement des lésions sur les différentes cabosses infectées dans les cacaoyères dans le Département d’Aboisso Sud-est de la Côte d’Ivoire. Méthodologie et résultats : Cinq plantations ont été choisies avec dans chacune vingt plants mis en observation et sur lesquels trois parties ont été délimitées : le bas, le milieu et les branches. Sur chaque cabosse infectée la lésion est soit, proximale, latérale ou distale. Le dispositif est donc en blocs de Fischer avec un arrangement factoriel, 2 facteurs (localisation des cabosses dans l’arbre et la position des lésions sur les cabosse) avec chacun 3 niveaux soit, 9 traitements avec 5 répétions. L’analyse des taux des affections à Phytophthora donne une différence hautement significative d’une part entre les localisations des cabosses dans l’arbre et d’autre part, entre les positions des lésions sur les cabosses. Aussi, la position des lésions sur les cabosses diffère-t-elle selon la localisation de celles-ci dans l’arbre (p-value < 1%). Conclusion : La pourriture brune sévit de façon endémique dans le département d’Aboisso. Les cabosses sur le tronc, notamment celles qui sont vers le bas, s’avèrent les plus vulnérables. Les lésions distales sont préférentiellement situées sur les cabosses vers le bas du tronc, alors que les proximales sont en grand nombre sur le milieu du tronc et les branches. Les Phytophthora étant des organismes telluriques, l’importance de leurs attaques sur les cabosses et sur les parties de celles-ci proches du sol, source d’inoculum primaire s’avérerait inévitable. Application actuelle et potentielle des résultats : A partir de ces résultats, il convient de recommander d’éviter l’abandon de la protection avec des fongicides appropriés de la cacaoyère de cette localité comme ont tendance à le faire certains planteurs. Aussi, les applications de fongicides, surtout ceux de contact, par atomisation ne doivent-ils pas être dirigées seulement dans les branches mais également sur le tronc et surtout vers le bas des pieds des arbres. Mots clés : pourriture brune, dynamique, cacaoyer, Aboisso, Côte d’Ivoire ABSTRACT Spatio-temporal dynamics of the brown rot of cocoa pots due to phytophthora sp. in the department of Aboisso (Cote d'Ivoire) Objective: This study was conducted to evaluate the level of brown rot, monitor its evolution in plantations, at the level of different parts of the cocoa tree and to specify the location of the lesions on the different pods infected in the cocoa farms in the Department. of Aboisso South-east of Ivory Coast. Methodology and results: Five plantations were selected, each of which had twenty plants under observation and on which three parts were delineated: the bottom, the middle and the branches. On each infected pod the lesion is proximal, lateral or distal. The device is therefore in Fischer blocks with a factorial arrangement, 2 factors (location of the pods in the tree and the position of the lesions on the pod) with each 3 levels, i.e. 9 treatments with 5 repetitions. The analysis of the Phytophthora disease rates gives a highly significant difference between the location of the pods in the tree and between the positions of the lesions on the pods. Also, the position of the lesions on the pods differs according to the location of these in the tree (p-value <1%). Conclusion: Brown rot is endemic in the Aboisso department. Pods on the trunk, especially those that are down, are the most vulnerable. The distal lesions are preferentially located on the pods down the trunk, while the proximal ones are in large numbers on the middle of the trunk and branches. Phytophthora being telluric organisms, the importance of their attacks on pods and parts of them that are close to the soil, a source of primary inoculum, would be inevitable. Current and Potential Application of Results: From these results, it is advisable to avoid abandoning the protection with appropriate fungicides of the cocoa farm in this locality, as some planters tend to do. Also, the applications of fungicides, especially those of contact, by atomization should not be directed only in the branches but also on the trunk and especially down the feet of the trees. Keywords: brown rot, dynamics, cocoa, Aboisso, Ivory Coast

Author(s):  
Konan G. S. N'zi ◽  
Jules E. H. Ayekoue ◽  
Yapi G. Yaye ◽  
Marie F. N'guessan ◽  
Ako A. B. Ako ◽  
...  

Background: The testis-specific serine/threonine protein kinase (TSSK2) is an indispensable protein responsible for the mobility of spermatozoa expressed specifically in the germ cells during spermatogenesis and present in the mature spermatozoa. Its gene mutation could constitute a risk of infertility. The aim of this study is to investigate the polymorphism of this TSSK2 gene in men with asthenozoospermia.Methods: The ejaculates were obtained from patients attending the reproductive biology unit of Institut Pasteur of Côte d’Ivoire for their spermiological evaluations. The semen analyses are performed with the automatic sperm analyzer SQA-Vision. 30 sperms, including 20 asthenozoosperms and 10 normosperms, were selected from their spermiological results and the spermatozoa DNA was extracted by the phenol/chloroform method. Direct Sequencing of the spermatozoa DNA fragments was done using the Sanger method. The frequencies of mutation were analysis with the Fisher and Mann-Whitney tests.   Results: It was revealed 17 mutations in 22 ejaculates. The frequent mutations are c.839C>T (T280M), c.816G>C (L372L), c.1026G>A (R342R), c.785A>C (H262P) and c.80A>G (K27R) with respectively frequencies of 50.0%, 26.67%, 16.67%, 13.33% and 10.0%. The analysis of these mutations indicated a significant difference in the frequency of occurrence of mutations between normosperms and asthenozoosperms (p-value = 0.01).Conclusions: This study shows that mutations in the TSSK2 gene are more common in asthenozoosperm ejaculates than normosperm ejaculates. This fact suggests the probable association of mutations in the TSSK2 gene with asthenozoospermia.


2020 ◽  
Vol 16 (3) ◽  
Author(s):  
Sidibe Oumane ◽  
Kouassi Kouadio Henri ◽  
Kouassi Konan Edouard

Author(s):  
Daniel Kouamé Kra ◽  
Yapi Richmond Baka ◽  
David Coulibaly N’golo ◽  
Ipou Joseph Ipou

The cocoa tree, the mainexport crop in Côte d'Ivoire is frequently attacked by a disease: brown pod rot, caused by Phytophthora spp. which causes a considerable drop in production. This soil-borne pathogen attacks on so-called weeds when environmental conditions are favourable. The presence of these susceptible weed hosts can amplify this scourge of brown rot by transmitting the pathogen to the crop plant. In order to improve the yield of this crop, a study was conducted in the Nawa region to identify the weed hosts through the characterization of the pathogen. This study consisted first in the inventory of susceptible host weeds of Phytophthora spp. and second in the morphological characterization of the pathogen. Concerning the host weeds inventory we procceeded determining all symptomatic weeds located within 3 m of the cocoa plants affected by brown rot. We identified 48 susceptible host species, divided into 41 genera and 22 families and we observed brown spots on the leaves of Oplismenus burmannii and Laportea aestuans; on the leaves of Clerodendrum splendens and Xanthosoma mafaffa, brown necroses surrounded by a yellow halo were noticed. Oily brown spots were examined on the leaves of Ageratum conyzoides. On the proximal, lateral and distal parts of the pods symptoms of brown spots covered with whitish mycelia were observed. Phytophthora strains obtained from the pods and weeds on PDA culture medium were characterized by matted, white mycelial colonies with a cottony appearance. Microscopic features revealed the presence of sporocysts, oospores and chlamydospores of different shapes.


Author(s):  
Kouamé Désiré ◽  
Biego Henri Marius ◽  
Niamketchi Gilles Léonce ◽  
Konan Ysidor ◽  
Sidibé Daouda

Aims: Maize (Zea mays L.) is a major staple food for millions of people in Côte d’Ivoire. Due                         to its high productivity and low cost of calorie it is preferred crop for food security of the                      country. Thus, this study was conducted to assess nutritive quality of maize produced and               stocked in five purposively selected regions of Côte d’Ivoire which represents five agroecological settings. Study Design: A total of 1500 samples of maize as grains, epis and spathes were collected at rate of 500 samples by region (Gbêkê, Poro, Hambol, Indénié-Djuablin and Gontougo) and sent to the laboratory in order to analyse their nutritional quality. Place and Duration of Study: This study was carried out during March 2016 to January 2017. The collected sample were carried out at the laboratory unit of Food Sciences and Biochemistry of the Félix Houphouët-Boigny University, Abidjan. Methodology: Proximate analyses were carried out using standard methods AOAC (2000). Results: The results show significant difference from the biochemical compositions of maize type and region. Mean value intervals were as follow: dry matter (85.83 – 91.42%), ash (1.19 - 2%), proteins (7.99 - 9.32), lipids (3.21 - 4.47), carbohydrates (71.80 - 77.94), starches (62.30 - 68.44%), fibers (5.03 - 5.83%), total sugars (2,13 - 2.99%), reducing sugars (0.33 - 0.66%), free fat acidity (1.86- 4.50%), peroxide value (1.34 - 3.07 meq O2/kg), iodine value (100.93 - 130.56 g I2/100 g), unsaponifiable (0.89 - 1.54%) and energy values (357.88 - 374.39 kcal). Conclusion: A significant variability from one region to another can be noticed at level of maize quality regardless the type of maize. The nutritive quality of maize seems to be tied to postharvest treatments (drying), type of storage (epis, grains and spathes) and structure of storage.


Hydrology ◽  
2018 ◽  
Vol 5 (3) ◽  
pp. 32
Author(s):  
Coulibaly Naga ◽  
Coulibaly Talnan Jean Honoré ◽  
Ochou Delfin ◽  
Yapo Bernard ◽  
Zabi Guillaume ◽  
...  

2010 ◽  
Vol 7 (1) ◽  
Author(s):  
D Diomande ◽  
T Tie-Bi ◽  
E Franquet ◽  
A Maasri ◽  
A Ouattara ◽  
...  

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