scholarly journals Comparative diagnostics of Cattle leukemia by ELISA method in test kits of various constructions

2019 ◽  
pp. 32-36
Author(s):  
B. T. Stegniy ◽  
A. I. Zavgorodniy ◽  
S. K. Gorbatenko ◽  
O. M. Kornieikov ◽  
M. Yu. Stegniy ◽  
...  
Keyword(s):  
False Positive ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Antibody Test ◽  
Virus Antibody ◽  
Specific Antibodies ◽  
Test Kit ◽  
Diagnostic Capacity ◽  
Cattle Blood ◽  
Positive Results

The purpose of the work was to carry out comparative analysis of the positive and negative on leukemia cattle blood sera in ELISA kits of different constructions. Research was carried out using “DIA®-BLV-Ab” kit, in which the reaction had been performed in the indirect ELISA, and “ID Screen® BLV Competition” kit in a competitive format. There were used 15 cattle blood sera for testing, in which antibodies to BLV were confirmed in the ID and the ELISA “Bovine leukemia virus antibody test kit” (IDEXX), as well as 10 positive cattle blood sera confirmed in ID, 10 weak positive sera tested in ID and 10 sera with a weak line of precipitate in ID, 34 negative for leukemia blood sera tested in ID, from which 24 were also tested in the ELISA “Bovine leukemia virus antibody test kit”. The “DIA®-BLV-Ab” kit and “ID Screen® BLV Competition” kit determined positive 25 blood sera with antibodies to BLV, which were positive in ID, and 15 samples were also confirmed in IDEXX test kit. When analyzing 10 sera, that were weak positive in ID, the “DIA®-BLV-Ab” kit determined 8 sera as positive and 2 samples as negative. The “ID Screen® BLV Competition” kit detected specific antibodies to all sera. When analyzing 14 sera with a weak precipitate line in ID, the “DIA®-BLV-Ab” kit determined 9 samples as positive and 5 as negative. The “ID Screen® BLV Competition” determined specific antibodies in 11 samples When analyzing 3 sera, the test result was negative in both ELISA kits. The “DIA®-BLV-Ab” kit determined as negative all 34 sera, which were negative in ID, 24 samples from them were negative in IDEXX test kit. In the “ID Screen® BLV Competition” kit 5 false positive results were received. Studies have shown that both test kits have a high diagnostic capacity and detect antibodies to BLV at different concentrations in all positive sera. The “DIA®-BLV-Ab” kit determined 34 sera as negative, in which specific antibodies were absent, and the “ID Screen® BLV Competition” kit identified 5 samples with a false positive result

scholarly journals Evaluation of a serum ELISA for detection of bovine leukemia viral antibodies in milk samples

2019 ◽  
Vol 31 (4) ◽  
pp. 598-600
Author(s):  
James F. Evermann ◽  
David M. DeAvila ◽  
Steven M. Parish ◽  
Catherine H. Merritt ◽  
Katherine C. Noble ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Antibody Test ◽  
Serum Samples ◽  
Milk Samples ◽  
Lactating Cows ◽  
Fresh Milk ◽  
Worldwide Distribution ◽  
Test Kit ◽  
Beef Herds

Bovine leukemia virus (BLV) infection has worldwide distribution in both dairy and beef herds. Our study was initiated in order to encourage control of BLV infection by using milk samples, in lieu of serum samples, to readily test lactating animals prior to dry-off and calving. Two Holstein dairy herds (A and B), with known status of BLV infection as determined by serology, were sampled by the collection of serum and fresh milk samples. Selected samples were tested using a USDA-licensed BLV antibody ELISA kit (Bovine leukemia virus antibody test kit; VMRD, Pullman, WA) for serum. Forty-one lactating cows from each herd were sampled. Herd A was confirmed to have endemic BLV infection; herd B was confirmed to be free of BLV infection. The milk ELISA results demonstrated 100% identification of positive and negative animals compared with the serum results. The correlation of the ELISA values between serum and milk samples was 97%, which supports the use of this BLV ELISA on milk samples.

scholarly journals Comparative research of diagnostic efficiency of ELISA test systems of domestic and foreign production for Animal brucellosis diagnostics

2019 ◽  
pp. 63-68
Author(s):  
B. T. Stegniy ◽  
S. S. Drahut ◽  
V. A. Kutsenko ◽  
T. P. Ramazanova ◽  
N. V. Marchenko ◽  
...  
Keyword(s):  
Positive Result ◽  
Francisella Tularensis ◽  
False Positive ◽  
Elisa Test ◽  
Farm Animals ◽  
Specific Antibodies ◽  
Milk Samples ◽  
Maximum Value ◽  
Test Kit ◽  
Positive Results

The purpose of the work. Comparison the diagnostic ability of the ELISA test kits «DIA®-Brucella ab. combi-V» and «ID Screen® Brucellosis Serum Indirect Multi-species» for the detection of antibodies to brucellosis pathogens in various farm animals. Materials and methods. For the analysis there were used 29 positive samples to brucellosis with specific antibodies in different concentrations, 26 of which are serums (22 — from cattle, 2 — from pigs, 1 — from goat, 1 — from camel) and 3 — milk samples from cows. There were used 32 serums (23 — from cattle, 6 — from sheep, 2 — from pigs, 1 — from goat), and 2 milk samples from cows that don’t contain antibodies to brucellosis pathogens for determining the ability of test kits to detect correctly negative samples. There were also used serums from cattle containing antibodies that can lead to false positive results, 1 sample with antibodies to Francisella tularensis, 1 — to Yersinia 03 and 1 — to Yersinia 09. To compare the results in the two test kits, comparative ratios were used that allowed to determine how many times the result obtained in both test kits was higher or less than cut off, that differentiated positive samples from negative. Results of the work. When analyzing 22 cattle serums containing antibodies to B. abortus, the “DIA®-Brucella ab. combi-V” kit determined all samples positive with a results 5.3–10.6 times higher than cut off. The “ID Screen® Brucellosis Serum Indirect Multi-species” test kit identified only 18 positive serums with a maximum value of 1.3 above the cut off. The result of the analysis of 3 samples was doubtful and 1 serum was negative. When analyzing 4 sera from different animals containing antibodies to brucellosis pathogens, the “DIA®-Brucella ab. combi-V” test kit identified all positive samples with the results 8.1–9.4 times higher than cut off. The “ID Screen® Brucellosis Serum Indirect Multi-species” test kit detected specific antibodies in only 3 serums — from pigs and camel. When the goat serum was tested, a doubtful (uncertain) result of the analysis was obtained. When analyzing 3 milk samples from cows containing antibodies to B. abortus in different concentrations there was received a positive result to brucellosis in both test kits. However, ability of the “DIA®-Brucella ab. combi-V” test kit to detect specific antibodies was significantly higher than in comparison test kit. When investigating 32 serums from different animals and 2 milk samples that didn’t contain antibodies to the brucellosis pathogens, a negative result of the analysis was obtained in both test kits. When analyzing cattle serums containing antibodies that can lead to false positive results, both test kits identified 1 sample with antibodies to Francisella tularensis and 1 serum with antibodies to Yersinia 03 with negative result. When analyzing 1 serum with antibodies to Yersinia 09 the result of the analysis was false positive. Conclusions. Studies have shown that the “DIA®-Brucella ab. combi-V” test kit has a high diagnostic capacity. When analyzing 29 blood serums, including samples from different animals, and milk samples from cows containing antibodies to brucellosis pathogens, the test kit identified all samples as positive with results 5.3–10.8 times above the cut off. The “ID Screen® Brucellosis Serum Indirect Multi-species” test kit detected antibodies to brucellosis pathogens only in 24 samples with a maximum value 1.3 times higher than cut off. When investigating 4 serums, 3 samples of which are from cattle and 1 — from goat, the result of the analysis was doubtful (uncertain), 1 cattle serum was identified as negative. The ability of test kits to detect correctly negative samples was comparable. When analyzing 32 serums from different animals and 2 milk samples from cows that do not contain antibodies to brucellosis pathogens, in both test kits, a negative result of the analysis was obtained. For the 3 negative cattle serums, the analysis of which on brucellosis may be incorrect (the presence of antibodies to Yersinia О3, Yersinia О9, Francisella tularensis), in both test kits, for 1 sample with antibodies to Yersinia О9 a false positive result was obtained

Incidence of bovine leukemia virus-specific antibodies in west african cattle

1986 ◽  
Vol 37 (4) ◽  
pp. 619-621 ◽  
Author(s):  
Francoise Walrand ◽  
Francis Fumoux ◽  
Georges Roelants ◽  
André-Laurent Parodi ◽  
Daniel Levy
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
West African ◽  
Specific Antibodies ◽  
African Cattle

scholarly journals AVIAN INFLUENZA ANTIBODY RESPONSE IN PIGLETS THATAREGIVEN ESCHERICHIA COLI–AVIAN INFLUENZA VACCINES

2021 ◽  
Vol 10 (1) ◽  
pp. 61-70
Author(s):  
Audrey Febiannya Putri Bhaskara ◽  
I Gusti Ngurah Kade Mahardika ◽  
I Nyoman Suartha
Keyword(s):  
Escherichia Coli ◽  
Avian Influenza ◽  
Optical Density ◽  
Antibody Test ◽  
Influenza Vaccines ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Antibody ◽  
E Coli ◽  
Test Kit ◽  
Virus Influenza

Babi berperan penting dalam ekologi virus influenza, karena babi dapat berperan sebagai wahana untuk reasorsi virus influenza dari unggas dan mamalia. Vaksinasi dengan antigen influenza universal, yaitu nukleoprotein, dapat menurunkan peluang babi dalam memunculkan virus influenza baru. Penelitian ini bertujuan untuk mengentahui respons antibodi dari vaksinasi dengan nukleoprotein rekombinan - Escherichia coli. Sebanyak 12 anak babi landrace dari tiga induk yang berbeda dipilih secara acak. Enam ekor divaksinasi dengan vaksin nucleoprotein-E. coli pada umur tujuh hari dan diulang pada umur 21 hari. Enam ekor tidak divaksinasi. Serum diambil pada umur 35 hari. Nilai optical density (OD) antibodi terhadap nukleoprotein diuji dengan teknik Enzyme-Linked Immunosorbent Assay (ELISA) dengan menggunakan Kit ELISA komersial, Avian Influenza Virus Antibody Test Kit. Hasil penelitian menunjukkan bahwa nilai Optical Density rata-rata babi yang divaksinasi (0,367) secara statistika nyata lebih tinggi dibandingkan dengan yang tidak divaksinasi (0,054). Vaksin rekombinan nucleoprotein-E. coli yang dicobakan mampu meningkatan antibodi terhadap virus avian influenza pada anak babi.

scholarly journals Detection of Specific Antibodies to an Antigenic Mannoprotein for Diagnosis of Penicillium marneffeiPenicilliosis

1998 ◽  
Vol 36 (10) ◽  
pp. 3028-3031 ◽  
Author(s):  
Liang Cao ◽  
Da-Liang Chen ◽  
Cindy Lee ◽  
Che-Man Chan ◽  
King-Man Chan ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Blood Donors ◽  
Antibody Test ◽  
High Specificity ◽  
Pathogenic Fungi ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Immunodeficiency Virus ◽  
Positive Results

The disseminated and progressive fungal disease Penicillium marneffei penicilliosis is one of the most common infectious diseases in AIDS patients in Southeast Asia. To diagnose systemic penicilliosis, we developed an enzyme-linked immunosorbent assay (ELISA)-based antibody test with Mp1p, a purified recombinant antigenic mannoprotein of P. marneffei. Evaluation of the test with guinea pig sera against P. marneffei and other pathogenic fungi indicated that this assay was specific for P. marneffei. Clinical evaluation revealed that high levels of specific antibody were detected in two immunocompetent penicilliosis patients. Furthermore, approximately 80% (14 of 17) of the documented penicilliosis patients with human immunodeficiency virus tested positive for the specific antibody. No false-positive results were found for serum samples from 90 healthy blood donors, 20 patients with typhoid fever, and 55 patients with tuberculosis, indicating a high specificity of the test. Thus, this ELISA-based test for the detection of anti-Mp1p antibody can be of significant value as a diagnostic for penicilliosis.

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