This essay reframes and reconceives gender as both a public feeling (in Cvetkovich’s sense of the term) and an affective assemblage. The latter concept, which extends the former, is designed to accommodate the multiplicity of factors, forces, processes, and agencies implicated in gender in general, but in non-normative gender in particular. The essay’s affective assemblage is eclectically composed from Deleuzoguattarian philosophy, pheonomenology, new materialisms, and affect theory, and enacted in the limit case of non-transitioning transgendered people in online communities. Gender as an affective assemblage takes a theological turn in the essay’s concluding section where it counters a territorialized reading of Christ’s body, one which seeks to exclude non-normative genders from the church. Calling for the church’s self-deterritorialization, the essay proposes a corporate body enfleshed by queer affective assemblages that would facilitate gendered exploration and discovery.