scholarly journals MICROWAVE-ASSISTED EXTRACTION OF INULIN FROM JERUSALEM ARTICHOKE AND PARTIAL ACID HYDROLYSES

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Alabadi & Abood
Keyword(s):  
Qualitative Analysis ◽  
Acid Hydrolysis ◽  
Jerusalem Artichoke ◽  
Microwave Assisted Extraction ◽  
Optimum Conditions ◽  
Microwave Assisted ◽  
Ph Values ◽  
Rp Hplc ◽  
Assisted Extraction ◽  
Hydrolysis Of

The Recent study aimed to identify the optimum conditions for inulin extraction from jerusalem artichoke tuber (JAT) powder using microwave assisted extraction method, and partially hydrolysis of purified inulin for producing fructooligosaccharide (FOS) using citric acid & microwave energy. The extraction conducted at different temperature (90 – 95) C˚ at (700 W/5min, 450w/8 min and 350w/14 min). The extracted inulin was concentrated to (50 – 60) % of original volume using rotary evaporator, and purified by lime method. The purified inulin extract was concentrated to 32 brix and mixed with acetone (3:1 acetone : extract), kept for 24 h at 4 C˚, then centrifuged at 10000g / 15 min., the precipitate dried at 55 ˚C. Acid hydrolysis of purified inulin carried out at different pH values (1.5, 2.5 & 3.0) at (90 ±2) ˚C. Aliquot of inulin hydrolysate were taken after (5, 10, 15, 20, 25 & 30) min. and subjected to qualitative analysis by RP-HPLC and TLC. The obtained results indicated that the microwave assisted extraction at 700 W/ 5 min. / 95 ˚C was superior as compared to the rest. The yield of extracted inulin was about 39.61 % and the extraction efficiency was 94.31 %, while the optimum condition for inulin acid hydrolysis appeared to be at 90 ˚C/ 15 min at pH 2.5 and 450 W. The qualitative analysis using (RP-HPLC & TLC) showed that the DP of inulin units ranged from (2 - 35) and for FOS ranged from      (2 – 9) unit.      

scholarly journals EXTRACTION AND ACID HYDROLYSES OF FRESH JERUSALEM ARTICHOKE INULIN FOR FRUCTOOLIGOSACCHARIDE PRODUCTION

2019 ◽  
Vol 50 (6) ◽  
Author(s):  
Alabadi & Abood
Keyword(s):  
Qualitative Analysis ◽  
Acid Hydrolysis ◽  
Extraction Efficiency ◽  
Jerusalem Artichoke ◽  
Degree Of Polymerization ◽  
Hot Water ◽  
Hot Water Extraction ◽  
Ph Values ◽  
Rp Hplc ◽  
Hydrolysis Of

The objective of this study was to produce fructooligosaccharide (FOS) from fresh jerusalem artichoke (JAT) inulin which was extracted by conventional method (hot water extraction) at two different temperature (80˚C & 90˚C\ 90 min). JAT slices were mixed with distilled water at (1 : 5) (w : v) ratio, the obtained extract purified and concentrated to 32˚Brix, then mixed with acetone (1 : 3) (v : v), kept at 4˚C for 24 h., centrifuged at 10000g/ 15 min., and the precipitate dried at 55˚C. Acid hydrolysis of the dried inulin was carried out at three different pH values being (1.5, 2.5 & 3.0) at 85˚C using citric acid (10 %), aliquot of inulin hydrolysate were taken after (5, 10, 15, 20, 25 & 30) min. for qualitative analysis using TLC and RP-HPLC. The results revealed that there were no significant differences in the yield of extracted inulin (33.86, 33.67 %) and extraction efficiency (80.62, 80.17 %) between the extraction at 80˚C and 90˚C\ 90 min. respectively. The optimum condition for acid hydrolysis of the obtained inulin was at pH 2.5 after 15 min at 85˚C. The qualitative analysis for hydrolysate using TLC and RP-HPLC showed that the degree of polymerization (DP) of inulin ranged from (2 - 35) unit and for FOS ranged from (2 – 9) units.   

scholarly journals DETERMINATION OF CHLORPYRIFOS IN LETTUCE (LACTUCA SATIVA L.) BY MICROWAVE-ASSISTED EXTRACTION COUPLED WITH HEADSPACE SINGLE DROP MICROEXTRACTION

2017 ◽  
Vol 9 (5) ◽  
pp. 248
Author(s):  
Naruephon Watthanaphap ◽  
Surapol Natakankitkul ◽  
Nuchnart Jonglaekha
Keyword(s):  
Ph Value ◽  
Limit Of Detection ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Optimum Conditions ◽  
Single Drop ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Single Drop Microextraction ◽  
Limit Of Quantitation

Objective: The objective of this research was to improve the method of extracting pesticide residue from vegetable by using microwave-assisted extraction, headspace single drop microextraction (MAE-HS-SDME) and analysed by gas chromatograph-mass spectrometer (GC-MS).Methods: Microwave-assisted extraction (MAE) coupled with headspace single drop microextraction (HS-SDME) was used for extraction of chlorpyrifos insecticide residues in lettuce. The optimum conditions for obtaining efficient extraction have been tried; kind and quantity of solvent, extraction time, microwave irradiation power, pH value and the amount of salt added in the sample followed the method of Plackett–Burman. Optimum conditions for efficient analysis were evaluated by using a central composite design (CCD) combined with a response surface methodology (RSM).Results: The optimum extract conditions from CCD for the MAE-HS-SDME method were: using 1.5 µl of n-butyl acetate, 460 W of the microwave power, 4.5 min of the extraction time, no pH adjustment and no salt addition. Verification of the optimized experimental conditions showed that validation of the determination coefficient ranges from the concentrations of 0.01 to 0.10 mg/l, equal to 0.999; the percentages of variation coefficient were ranges from 4.6 to 7.7%; the recoveries ranges from 70.0 to 79.5%; limit of detection was 0.003 mg/kg and limit of quantitation was 0.01 mg/kg.Conclusion: In this study, the MAE-HS-SDME method coupled with GC-MS was found most suitable for chlorpyrifos analysis. The results obtained from analyzing chlorpyrifos in lettuce samples indicated that this method is rapid, simple, sensitive, reducible cost and safety for the analyst and the environment.

scholarly journals Microwave-Assisted Extraction of Herbacetin Diglucoside from Flax (Linum usitatissimum L.) Seed Cakes and Its Quantification using an RP-HPLC-UV System

Molecules ◽  
2014 ◽  
Vol 19 (3) ◽  
pp. 3025-3037 ◽  
Author(s):  
Ophélie Fliniaux ◽  
Cyrielle Corbin ◽  
Aina Ramsay ◽  
Sullivan Renouard ◽  
Vickram Beejmohun ◽  
...  
Keyword(s):  
Linum Usitatissimum ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Linum Usitatissimum L ◽  
Rp Hplc ◽  
Assisted Extraction

scholarly journals Inulin obtained from Jerusalem artichoke using microwave-assisted extraction and its metabolic influence

2021 ◽  
Vol 56 (4) ◽  
pp. 477-487
Author(s):  
Manuel DRUGULESCU ◽  
◽  
Cristina Elena DINU-PIRVU ◽  
Narcisa BABEANU ◽  
◽  
...  
Keyword(s):  
Jerusalem Artichoke ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction
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