scholarly journals MOLECULAR PHYLOGENY OF GALL-FORMING APHIDS TRIBE FORDINI (HOMOPTERA, APHIDIDAE, ERIOSOMATINAE) ON PISTACIA TREES FROM KURDISTAN REGION NORTH OF IRAQ

2020 ◽  
Vol 51 (Special) ◽  
Author(s):  
Ali & et al.

 Four aphid species belonging to the Tribe Fordini (Homoptera, Aphididae,  Eriosomatinae) induce galls on Pistacia trees in North Iraq (Kurdistan) were recorded in this study. Molecular phylogeny of Iraq Fordini species was constructed for These species (Forda riccobonii, Paracletus  cimiciformis, Baizongia pistaciae and Forda marginata), based on sequences of mitochondrial Cytochrome Oxidase I (COI)  gene of these aphid species to detect  the relationship and taxonomy of  Iraqi species for tribe Fordini. Our results confirmed that each of Forda and Paracletus are a sister genus, or monophyletic group by designing in same clade, while B. pistaciae  is separate from other clades, supporting the taxonomic division of the tribe Fordini  into two subtribes (Fordina and Baizongina).

Zootaxa ◽  
2019 ◽  
Vol 4668 (3) ◽  
pp. 410-420
Author(s):  
CHAO WANG ◽  
YUNYUN GAO ◽  
THOMAS PAPE ◽  
DONG ZHANG

Sarcophaga Meigen, 1826 is proposed as a senior synonym of Cornexcisia Fan & Kano, 2000, syn. nov. and Fanzideia Xue, Verves & Du, 2011, syn. nov. Cornexcisia Fan & Kano, 2000, stat. rev. is given status as a subgenus and is considered a senior synonym of Fanzideia Xue, Verves & Du, 2011, syn. nov. at the subgeneric level. Cornexcisia is argued to contain S. (Cornexcisia) longicornuta (Fan & Kano, 2000), comb. nov., S. (C.) cygnocerca (Xue, Verves & Du, 2011), comb. nov., S. (C.) kurahashii (Shinonaga & Tumrasvin, 1979), subgen. comb. nov. (from Phallosphaera Rohdendorf) and S. (C.) suthep Pape & Bänziger, 2003, subgen. comb. nov. (from Rosellea Rohdendorf). Sarcophaga (C.) kurahashii is newly recorded from China (Yunnan), the male is redescribed and the female is described for the first time, supported by photographs, illustrations and mitochondrial cytochrome oxidase subunit I (COI) gene sequences. Species of Cornexcisia share an exceptionally long postpedicel in the female and the following apomorphic distiphallic appendages in the male: juxta ventro-proximally with an apically divided arm with cuticular pile, and lateral styli bifurcated from the base with each branch elongate, gently curved and slightly expanded apically. A key to the species of Cornexcisia is provided. 


Zootaxa ◽  
2009 ◽  
Vol 2200 (1) ◽  
pp. 61-68 ◽  
Author(s):  
ROGER D. PRICE ◽  
KEVIN P. JOHNSON

There are 23 species of Myrsidea recognized from passerine thraupid hosts. Five new species parasitic on members of this avian family are described. They and their type hosts are Myrsidea rozsai ex the Thick-billed Euphonia, Euphonia laniirostris d'Orbigny & Lafresnaye, M. cruickshanki ex the Carmiol's Tanager, Chlorothraupis carmioli (Lawrence), M. patersoni ex the Grey-headed Tanager, Eucometis penicillata (Spix), M. pagei ex the Crimson-backed Tanager, Ramphocelus dimidiatus Lafresnaye, and M. valimi ex the Tawny-capped Euphonia, Euphonia anneae Cassin. The first species is placed in the bonariensis species group, while the remaining four are placed in the fusca species group. Sequences of a portion of the mitochondrial cytochrome oxidase I (COI) gene were compared to other species of Myrsidea and were highly divergent.


Author(s):  
I Putu Sudiarta ◽  
Dwi Martiningsia ◽  
I Nyoman Wijaya

Some of fruit flies have been reported as the important pest on fruits and vegetables in the world. Agricultural Quarantine Agency Denpasar reported that there was new coming species (exotic) of fruit flies in Bali in 2014 based on the morphological identification, namely Bactrocera occipitalis. However Bactrocera dorsalis complex have similar morphological characters and have a less distinctive character for taxonomic identification, therefore it is difficult to identify fruit flies accurately. Based on that phenomena, the accurate identification is needed. One of the more accurate identification techniques is based on molecular identification using DNA-based barcode. To identify fruit flies, DNA-based barcode using mitochondrial cytochrome oxidase I (COI) gene has been conducted. PCR analysis using Fruit Fly MT-CO1-F (FFMT-CO1-F) 5’-GGAGCATTAATYGGRGAYG-3’ as forward primer and HCO 5’-TAAACTTCAGGGTGACCAAAAATCA-3’ as reverse primer was successfully amplified around 600 bp of COI gene of fruit flies. Based on similarity of sequence product, the species was identifiedas Bactrocera occipitalis and same result was revealed using morphological identification. Phylogenetic analysis of B. occipitalis based on COI genes showed that B. occipitalis from Bali were in the same groups with Bactrocera species from Tarakan and Philippines. In addition, Bactrocera occipitalis as exotic fruit fly is a new report in Bali, Indonesia.


2020 ◽  
Vol 44 (6) ◽  
pp. 508-518
Author(s):  
Sam PETER ◽  
Manoj Kumar BHASKARAN NAIR ◽  
Devika PILLAI

Chaetognaths (arrow worms) are an enigmatic group of transparent planktonic invertebrates and play an important role in the marine food web. Their morphological and developmental features have raised extensive debates since the discovery of the phylum in the 18th century. Uncertainty in the phylogenetic placement of certain chaetognath species still exists and is puzzling many scientists who have tried to clarify this task. Studies using a portion of both small subunit ribosomal ribonucleic acid (SSU rRNA) and large subunit ribosomal ribonucleic acid (LSU rRNA) genes when integrated with conventional taxonomy were contributed to resolve taxonomical issues in this group. Here we present the first phylogenetic study of Chaetognatha based on a portion of mitochondrial cytochrome oxidase I (COI) gene and compare our results with the earlier morphological and molecular evolutionary hypotheses. This study includes 16 extant species, representing 8 genera and 6 of which are among the 9 extant families. We recommend the following clade structure for the phylum: Aphragmophora comprising Sagittidae with Pterosagittidae and Krohnittidae included in the Sagittidae and Phragmophora comprising Eukrohniidae, Spadellidae, and Heterokrohniidae. Phylogenetic analyses also supported the division of Phragmophora into two monophyletic groups: the Monophragmophora and Biphragmophora. Moreover, Ctenodontina/Flabellodontina and Syngonata/Chorismogonata suborders were not validated. Precise phylogenetic investigations using various molecular markers and specimens from diverse regions are definitely needed to provide an exact evolutionary concept on this phylum.


Zootaxa ◽  
2011 ◽  
Vol 3097 (1) ◽  
pp. 1 ◽  
Author(s):  
MICHEL P. VALIM ◽  
ROGER D. PRICE ◽  
KEVIN P. JOHNSON

Three species of previously described Myrsidea from the birds Lochmias nematura obscurata Cabanis, Automolus ochrolaemus (Tschudi, 1844) (both Furnariidae) and Pachyramphus cinnamomeus Lawrence, 1861 (Cotingidae) are redescribed, including new host and geographical records. Five new species of Myrsidea from the Neotropics are described and illustrated. These species and their type hosts are: M. waterstoni n. sp. from Anabacerthia variegaticeps (Sclater), M. meyi n. sp. from Syndactyla subalaris (Sclater) (Furnariidae), M. dalgleishi n. sp. from Glyphorynchus spirurus (Vieillot) (Dendrocolaptidae), M. cicchinoi n. sp. from Rhynchocyclus olivaceus (Temminck) (Tyrannidae), and M. castroae n. sp. from Atlapetes albinucha gutturalis (Lafresnaye) (Emberizidae). Sequences of a portion of the mitochondrial cytochrome oxidase I (COI) gene for four of these new species were highly divergent from those of other species of Myrsidea.


2005 ◽  
Vol 95 (5) ◽  
pp. 401-408 ◽  
Author(s):  
M.M. Monti ◽  
A.G. Nappo ◽  
M. Giorgini

AbstractThe genus Encarsia Förster includes parasitoid species that are effective natural enemies of whitefly and armoured scale insect agricultural pests. Within this genus, several species groups have been recognized on the basis of morphological similarity, although their monophyly appears uncertain. It is often difficult to separate morphologically similar species, and there is evidence that some species could in fact be complexes of cryptic species. Their correct identification is fundamental for biological control purposes. Recently, due to unreliability of morphological characters, molecular techniques have been investigated to identify markers that differentiate closely related species. In this study, DNA variation in an ~900 bp segment of the mitochondrial cytochrome oxidase subunit I (COI) gene was examined by both sequencing and PCR–RFLP. Two pairs of species that are difficult to distinguish morphologically were analysed: Encarsia formosa Gahan and Encarsia luteola Howard, belonging to the luteola group, and two populations of Encarsia sophia (Girault & Dodd) from Pakistan and Spain, belonging to the strenua group, recently characterized as cryptic species. High sequence divergence and species-specific restriction patterns clearly differentiate both species pairs. Parsimony analysis of the nucleotide sequences was also performed, including Encarsia hispida De Santis (luteola group) and Encarsia protransvena Viggiani (strenua group). Two monophyletic clades supporting the two groups of species considered were resolved. The results of this study support the use of the COI gene as a useful marker in separating species of Encarsia, for which morphological differences are subtle. Moreover, the COI gene appears potentially useful for understanding phylogenetic relationships in this genus.


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