Faster evolution of a premating reproductive barrier is not associated with faster speciation rates in New World passerine birds

Why are speciation rates so variable across the tree of life? One hypothesis is that this variation is explained by how rapidly reproductive barriers evolve. We tested this hypothesis by conducting a comparative study of the evolution of bird song, a premating barrier to reproduction. Speciation in birds is typically initiated when geographically isolated (allopatric) populations evolve reproductive barriers. We measured the strength of song as a premating barrier between closely related allopatric populations by conducting 2339 field experiments to measure song discrimination for 175 taxon pairs of allopatric or parapatric New World passerine birds, and estimated recent speciation rates from molecular phylogenies. We found evidence that song discrimination is indeed an important reproductive barrier: taxon pairs with high song discrimination in allopatry did not regularly interbreed in parapatry. However, evolutionary rates of song discrimination were not associated with recent speciation rates. Evolutionary rates of song discrimination were also unrelated to latitude or elevation, but species with innate song (suboscines) evolved song discrimination much faster than species with learned song (oscines). We conclude that song is a key premating reproductive barrier in birds, but faster evolution of this reproductive barrier between populations does not consistently result in faster diversification between species.

Temporal trends in abundance and phenology of migratory birds across the Italian Alps during a 20-year period

Introduction: Bird migration across mountainous regions has been studied usually at single sites due to the difficulty in employing and support a multi-site sampling effort. This may affect inference on migration whose scale is larger that a single site. The Progetto Alpi has been monitoring post-breeding bird migration through the Italian Alps with a network of several ringing stations since 1997. Until 2017, 666,471 ringing data of 191 different species were collected. Methods: Phenology of bird migration in terms of date of capture and the related elevational distribution during the sampling season (August-November) were analysed for 69 species. For a subset of 45 species the inter-annual variation in phenology along with trends in the number of ringed birds and in the ratio between the number of juveniles and adults ringed, were also analysed. Results: Migration through the Italian Alps occurred differently between species, with heterogeneity across species in the median date of capture and their elevational distribution. No linear trends in phenology were detected. For four species an annual linear increase of the ringed individuals was detected, while for other four species an annual linear decrease of the captures was detected. For two species, an increase of the ratio between the number of the juveniles and the number of adults ringed was detected. Conclusions: The long-term standardized monitoring of post-breeding migration allowed us to consolidate and increase knowledge of bird migration through Italian southern Alps. The information gathered allowed us to define the temporal and elevation distribution of passerine birds crossing the mountainous area, and to test interannual trends in the number of captures. The main purpose of this work was to report on the information collected in twenty years of field activity, providing a contribution to the understanding this complex phenomenon. Further investigations and more in-depth analyses are necessary to understand how environmental factors and species-specific eco-physiological traits interact and affect migratory strategies of passerine birds in the Italian Alpine region.

Heterothermy in a Small Passerine: Eastern Yellow Robins Use Nocturnal Torpor in Winter

Torpor is a controlled reduction of metabolism and body temperature, and its appropriate use allows small birds to adapt to and survive challenging conditions. However, despite its great energy conservation potential, torpor use by passerine birds is understudied although they are small and comprise over half of extant bird species. Here, we first determined whether a free-living, small ∼20 g Australian passerine, the eastern yellow robin (Eopsaltria australis), expresses torpor by measuring skin temperature (Ts) as a proxy for body temperature. Second, we tested if skin temperature fluctuated in relation to ambient temperature (Ta). We found that the Ts of eastern yellow robins fluctuated during winter by 9.1 ± 3.9°C on average (average minimum Ts 30.1 ± 2.3°C), providing the first evidence of torpor expression in this species. Daily minimum Ts decreased with Ta, reducing the estimated metabolic rate by as much as 32%. We hope that our results will encourage further studies to expand our knowledge on the use of torpor in wild passerines. The implications of such studies are important because species with highly flexible energy requirements may have an advantage over strict homeotherms during the current increasing frequency of extreme and unpredictable weather events, driven by changing climate.

Salmonella enterica serovar Typhimurium from Wild Birds in the United States Represent Distinct Lineages Defined by Bird Type

Salmonella enterica serovar Typhimurium is typically considered a host generalist, however certain strains are associated with specific hosts and show genetic features of host adaptation. Here, we sequenced 131 S. Typhimurium strains from wild birds collected in 30 U.S. states during 1978-2019. We found that isolates from broad taxonomic host groups including passerine birds, water birds (Aequornithes), and larids (gulls and terns) represented three distinct lineages and certain S. Typhimurium CRISPR types presented in individual lineages. We also showed that lineages formed by wild bird isolates differed from most strains originating from domestic animal sources, and genomes from these lineages substantially improved source attribution of Typhimurium genomes to wild birds by a machine learning classifier. Furthermore, virulence gene signatures that differentiated S. Typhimurium from passerines, water birds, and larids were detected. Passerine isolates tended to lack S. Typhimurium-specific virulence plasmids. Isolates from the passerine, water bird, and larid lineages had close genetic relatedness with human clinical isolates, including those from a 2021 U.S. outbreak linked to passerine birds. These observations indicate that S. Typhimurium from wild birds in the U.S. are likely host-adapted, and the representative genomic dataset examined in this study can improve source prediction and facilitate outbreak investigation.

Conspicuous female plumage is common among open cup-nesting passerine birds

Alfred Russel Wallace hypothesized that the use of cavity or dome nests releases incubating birds from predation risk, and that this allows the evolution of conspicuous coloration in females. By this hypothesis, females that use open nests are subject to strong selection for crypsis. Here, we test the validity of Wallace’s proposed evolutionary correlation between nest type and conspicuous coloration in females across the largest avian radiation, the Passeriformes, using phylogenetic comparative methods. We also test an alternate hypothesis that cavity-nesting results in greater conspicuousness because competition for cavities is stronger than for other nest sites, and such competition can drive social selection on female plumage. By this hypothesis, dome-nesting females should generally be less conspicuous than cavity-nesting species. We found no support for Wallace’s hypothesis that concealed nests yield conspicuous plumage while open nests yield dull plumage, and some support for the social selection hypothesis in smaller-bodied, gregarious species. While our analyses do not support the core part of Wallace’s hypothesis, they corroborate his contention that evolutionary transitions in nest type are rare, indicating that nest types may influence macroevolutionary selective regimes for other traits.

Eastern Equine Encephalitis Virus Taxonomy, Genomics, and Evolution

Eastern equine encephalitis virus (EEEV; Togaviridae, Alphavirus) is an arthropod-borne virus (arbovirus) primarily maintained in an enzootic cycle between Culiseta melanura (Coquillett) and passerine birds. EEEV, which has the highest reported case- fatality rate among arbovirus in the Americas, is responsible for sporadic outbreaks in the Eastern and Midwest United States. Infection is associated with severe neurologic disease and mortality in horses, humans, and other vertebrate hosts. Here, we review what is known about EEEV taxonomy, functional genomics, and evolution, and identify gaps in knowledge regarding the role of EEEV genetic diversity in transmission and disease.

A Novel Locality for the Observation of Thousands of Passerine Birds during Spring Migration in Los Angeles County, California

Avian migration is a spectacular phenomenon, representing the annual movements of billions of birds globally. Because the greatest diversity and numbers of birds migrate at night, opportunities to observe active migration are rare. At a number of localities in North America, however, observers can quantify movements of many typically nocturnal migrants during daylight where they continue after dawn. Such locations have provided much information about species-specific phenology, status, and orientation during migration. Localities where morning flights of land birds can be observed are unevenly distributed, however, and are little reported along the Pacific coast. Here we describe a novel location for the observation of spectacular morning flights of nocturnal migrants during spring migration at Bear Divide, in the western San Gabriel Mountains, Los Angeles County, California. In two years of informal surveys at the site, we have recorded at least one morning with an estimated ~13,500 individual birds passing. Our preliminary analyses suggest that the peak of a species’ migration at Bear Divide is correlated with the latitude of a species’ breeding, being later in the spring as that latitude increases. Our data from Bear Divide provide an independent perspective on migration as quantified by local radar. Further work at this locality may help inform our knowledge of migration phenology and population trends.

To get sick or not to get sick—Trichomonas infections in two Accipiter species from Germany

Trichomonosis caused by the flagellate Trichomonas gallinae is one of the most important avian diseases worldwide. The parasite is localised in the oesophageal area of its host and mainly infects pigeon and dove species. During the last decade, a host expansion to passerine birds occurred, making the disease a potential threat for passerine predators as naïve host species. Here, we investigated the effect of the parasite on two Accipiter species in Germany which show a comparable lifestyle but differ in prey choice, the Northern goshawk (Accipiter gentilis) mainly hunting pigeons and the Eurasian sparrowhawk (Accipiter nisus) mainly feeding on passerines. We genetically identified the parasite strains using the Fe-Hydrogenase gene as marker locus and compared the incidence of parasite presence and clinical signs of trichomonosis between nestlings of the two Accipiter species. In total, we identified 14 strains, with nine strains unknown so far. There was a higher strain diversity and prevalence of Trichomonas spp. in goshawks than sparrowhawks (42.4% vs. 21.2%) whereas sparrowhawks when being infected more often displayed clinical signs of trichomonosis than goshawks (37.1% vs. 6.1%). Even though sparrowhawks were mainly infected with the finch epidemic strain and genetic data indicated some variation between isolates, no correlation with virulence could be detected. All in all, goshawks seem to be better adapted to Trichomonas infections, whereas to sparrowhawks, this is a novel disease with more severe manifestations, from individual morbidity to a higher risk of population decline caused by trichomonosis.

Impact of climate changes on the diurnal behaviour of some passerines in some selected habitats of central Punjab, Pakistan

Present paper provides information on the impact of climate changes on diurnal rhythms of the four passerines viz, house sparrow (Passer domesticus Linn.), rosy starling (Pastor roseus Linn.), tree swallows (Tachycineta bicolor Hirun.), and brown shrike (Lanius cristatus Linn.) for two years period in the four major agricultural habitats viz. Faisalabad, Sheikhupura, Toba Tek Singh and Khanewal of Central Punjab, Pakistan. Effects of climate catastrophe have been seriously recognized as important inhibitory factors for birds’ sustainable existence and conservation. Among the four habitats, present within 120 kilometre radius from Faisalabad district, observations were conducted weekly on active and passive foraging, and the roost exits and returns throughout the day. Weather changes viz. temperature, precipitation and relative humidity were critically recorded. Impacts of temperature were significant and resulted in decline of the active foraging for the four designated birds (β= -2.36, -1.93,-2.15 and -1.64) whereas, precipitation due to its lowered frequency, exerted negative influence on the house sparrow and brown shrike, but was non-significant for rosy starling and tree swallows. Overall, temperature and relative humidity (RH) were the foremost climate factors and largely reduced the foraging and roosting movements of the four passerine birds’. Nonetheless, effects of lowered rainfall for the four habitats throughout this study were non-significant.

The Evolution of Nest Site Specialization and its Macroevolutionary Consequences in Passerine Birds

A long-standing hypothesis in evolutionary biology is that the evolution of resource specialization can lead to an evolutionary dead end, where specialists have low diversification rates and limited ability to evolve into generalists. However, in recent years, advances in comparative methods investigating trait-based differences associated with diversification have enabled more robust tests of this idea and have found mixed support. Here we test the evolutionary dead end hypothesis by estimating net diversification rate differences associated with nest site specialization among 3,224 species of passerine birds. In particular, we test whether the adoption of hole-nesting, a nest site specialization that decreases predation, results in reduced diversification rates relative to nesting outside of holes. Further, we examine whether evolutionary transitions to the specialist hole-nesting state have been more frequent than transitions out of hole-nesting. Using diversification models that accounted for background rate heterogeneity and different extinction rate scenarios, we found that hole-nesting specialization was not associated with diversification rate differences. Furthermore, contrary to the assumption that specialists rarely evolve into generalists, we found that transitions out of hole-nesting occur more frequently than transitions into hole-nesting. These results suggest that interspecific competition may limit adoption of hole-nesting, but that such competition does not result in limited diversification of hole-nesters. In conjunction with other recent studies using robust comparative methods, our results add to growing evidence that evolutionary dead ends are not a typical outcome of resource specialization.