AUTOLOGOUS FULL THICKNESS SKIN GRAFT WOUND HEALING ON SPRAGUE DAWLEY RATS Reviewed From TGF-? Expression And Neutrophil Number

Abstract Introduction : Skin grafts are now one of treatment option in wound healing process that is always developing. TGF-bexpression and the number of neutrophils have an important role in healing skin graft wounds. Ozone (O3) has disinfecting properties that are effective in wound healing. Objective : Proving the effectiveness of Ozonated VCO for Full Thickness Skin Graft wound healing using parameter of TGF-b expression and neutrophil number. Method : This study is an experimental study with a post-test only design group of 40 Sprague Dawley rats performed autologous skin graft at the same time. Samples were divided randomly into 8 groups (K1 = without Ozonated VCO), (A1 = Ozonated VCO 50 mg / ml), (B1 = Ozonated VCO 100 mg / ml), (C1 = Ozonated VCO 200 mg / ml), ( K2 = without Ozonated VCO) (A2 = Ozonated VCO 50 mg / ml), (B2 = Ozonated VCO 100 mg / ml), (C2 = Ozonated VCO 200 mg / ml). Assessment of TGF-b expression and neutrophil number of tissue was performed by staining hematoxylin & eosin and immunohistochemistry on days 6 and 12 after skin graft. Results : There were significant differences (p <0.05) TGF-b expression and neutrophils number of tissue between the control group and the administration of Ozonated VCO doses of 50 mg / ml, 100 mg / ml and 200 mg / ml on days 6 and 12 post skin graft. Conclusion : The administration of Ozonated VCO effectively improve Full Thickness Skin Graft wound healing seen from macroscopic wounds, increase TGF-b expression and decrease the number of neutrophils.

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The Effectivity of Ozonated VCO on Healing Full Grafted Skin graft Study on Progenitor Cell Endothelial Proliferation and Angiogenesis in Sprague Dawley Mice

Medica Hospitalia : Journal of Clinical Medicine ◽

10.36408/mhjcm.v8i2.446 ◽

2021 ◽

Vol 8 (2) ◽

pp. 133-138

Author(s):

Rollin Hansen Sidauruk ◽

Renny Yuniati ◽

Djoko Handojo

Keyword(s):

Wound Healing ◽

Skin Graft ◽

Healing Process ◽

Wound Healing Process ◽

Full Thickness ◽

Sprague Dawley ◽

Full Thickness Skin Graft ◽

Accelerate Wound Healing ◽

Full Thickness Skin ◽

Thickness Skin

Abstract Background : Full thickness Skin graft is one technique to accelerate wound healing. EPC proliferation and new blood vessel formation have an important role in the success of Ful thickness Skin graft. Ozone content in Ozonated VCO has the ability to increase growth factors so that it can accelerate wound healing Aims : This study was aimed to prove the effects of ozonated vco in various doses in increasing EPC proliferation and angiogenesis in full thickness skin graft wounds. Methods : This research is an experimental study with randomized post test only with parallel group design. 40 Sprague Dawley rats performed autologous Skin grafts at the same time. Samples were randomly divided into 8 groups, two control groups (X1 and X5), and 6 other groups were given a multi-dose ozonated oil 50.6 mg / ml (X2, X6), 101.3 mg / ml (X3, X7) , 202.6 mg / ml (X4, X8). Conduct termination on day 6 and day 12. Examination of the number of EPC proliferation and the number of angiogenesis in each group. Data were analyzed by Saphiro Wijk, ANOVA, post hoc benferonni and pearsons Results : The number of EPC proliferation in groups X1, X2, X3, X4, X5, X6, X7, X8 has a mean of 6.2 + 0.837, 10 + 1.581, 13.80 + 2.049, 14.80+ 2,280, 9.40 + 2,074 , 13.20 + 1,483, 19,0 + 2,055 and 19.20 + 1,095.The number of Angiogenesis in groups X5, X6, X7, X8 has a mean of 9.20 + 1,095,14,60 + 2,881,17,40+ 3,286, 18,80 + 2,168,17,40 + 3,286,18.80+ 2,168. The administration of ozonated VCO dose of 202.6 mg / ml gave the best results in the amount of angiogenesis (p = 0.001) and the amount of angiogenesis (p=0,001).Correlation analysis between EPC proliferation and angiogenesis showed a significant correlation (p = 0.001 and r = 0.738). Conclusion : Ozonated oil can increase the number of EPC proliferation and angiogenesis in the wound healng process of Full thickness Skin graft. The ozone dose of 202,6 mg / ml showed the best effect on increase wound healing process. Keyword : Full thickness skin graft, Ozonated oil , EPC, Angiogenesis

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The role of ozonated Aloe vera oil in full-thickness skin defects: Macrophage count and epithelization length parameter

F1000Research ◽

10.12688/f1000research.25063.1 ◽

2020 ◽

Vol 9 ◽

pp. 1218

Author(s):

Ukio Salferius Tamba ◽

Muhamad Thohar Arifin ◽

Muhamad Nur ◽

Muflihatul Muniroh ◽

Neni Susilanigsih

Keyword(s):

Aloe Vera ◽

Control Group ◽

Full Thickness ◽

Sprague Dawley ◽

Control Group Design ◽

Keratinocyte Proliferation ◽

Skin Defects ◽

Full Thickness Skin ◽

Sprague Dawley Rats ◽

Thickness Skin

Background: Aloe vera has been reported to enhance keratinocyte proliferation and migration, and thereby accelerate wound healing. Ozone therapy is an alternative medicine with disinfecting properties and strong oxidative stress induction capacity. This study aimed to evaluate the effect of Aloe vera oil provided with different dosages of ozone in accelerating the healing rate comparing two-phases; inflammation (day 3) and proliferation (day 7) of full-thickness defect wounds in Sprague Dawley rats as an adjuvant treatment based on macrophage count and new epithelialization length. Methods: We conducted a study using a post-test only control group design, where a total of 50 Sprague Dawley rats were randomized into ten groups. Two control groups were administered Aloe vera oil (P1, P6), while gentamicin ointment was used in the other control group (P2, P7). Ozonated Aloe vera oil dose was used: 600 mg/ml (P3,P8), 1200 mg/ml (P4,P9), and 1800 mg/ml (P5,P10). Groups P1, P2, P3, P4, P5 were terminated on the 3rd day, while P6, P7, P8, P9, P10 were evaluated on the 7th day. Macrophage counts were assessed using a 100x magnification microscope, through hematoxylin-eosin (HE) staining, and paraffin block with Masson trichrome staining was used to measure the new epithelialization length. Results: There were significant differences in the macrophage count on day three between the group-administered gentamicin (P2) and 1800 mg/ml ozonated Aloe vera oil (P5) (79.96;131.96, p<0,05). The new epithelialization length significantly increased in the group provided with wound treatment up to day 7, using 1800 mg/ml ozonated Aloe vera oil (P10), compared to non-ozonated Aloe vera (P6) and the gentamicin therapy (P7) (1160.88 µm; 1367.64; 2451.40 μm, p<0,05). Conclusions: The application of ozonated Aloe vera oil to full-thickness skin defects in Sprague Dawley rats resulted in a higher macrophage count and longer new epithelialization length than in controls.

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Effects of Caffeine Consumption on Autologous Full-Thickness Skin Graft Healing in an Animal Model

Indian Journal of Plastic Surgery ◽

10.1055/s-0041-1734573 ◽

2021 ◽

Author(s):

Tommy Supit ◽

Neni Susilaningsih ◽

Awal Prasetyo ◽

Keyword(s):

Wound Healing ◽

Skin Graft ◽

Collagen Synthesis ◽

High Dose ◽

Full Thickness ◽

Caffeine Consumption ◽

Full Thickness Skin Graft ◽

Full Thickness Skin ◽

Thickness Skin ◽

Medium Dose

Abstract Background There exists contradictory evidence that states both the beneficial and deleterious effects of caffeine on wound healing. The general population might unknowingly consume caffeine that negatively affects wound healing. The main objective of this study is to investigate the effect of daily caffeine consumption on wound healing, specifically full-thickness skin graft (FTSG). Methods Forty Sprague-Dawley rats were randomized into four groups of equal size: control-dose (CD), low-dose (LD), medium-dose (MD), and high-dose (HD) caffeine groups. After autologous FTSG, all subjects in the intervention group were given daily pure caffeine gavage. The FTSG was explanted 7 days posttransplant. The graft viability, secondary contraction, and adherence were evaluated macroscopically, while fibroblast and collagen deposition was analyzed microscopically with hematoxylin eosin stain. Results The least graft viability (72.8 ± 20.7%, clinical wound assessment scale [CWAS] 2.4), highest secondary contraction (11.4 ± 10.5%), and fibroblast count (331.8 ± 88.6 cells/5 high power fields) were observed in the MD group. More collagen synthesis was observed in subjects who consumed caffeine. The level of secondary contraction, fibroblast count as well as graft viability and collagen synthesis were positively correlated. Conclusions Daily consumption of caffeine impairs graft viability when given in medium dose and increases collagen synthesis, irrespective of dosage. This study was in experimental rats; the results are not directly translatable to humans.

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Ozonated aloe vera oil improve wound healing by decreasing neutrophil count and wound diameter in Sprague dawley rats with full-thickness skin defect

Indonesia Journal of Biomedical Science ◽

10.15562/ijbs.v14i2.253 ◽

2020 ◽

Vol 14 (2) ◽

pp. 48

Author(s):

Rezah Vahlepi ◽

Muhamad Thohar Arifin ◽

Muhammad Nur ◽

Muflihatul Muniroh ◽

Neni Susilaningsih

Keyword(s):

Wound Healing ◽

Neutrophil Count ◽

Aloe Vera ◽

Skin Defect ◽

Full Thickness ◽

Sprague Dawley ◽

Full Thickness Skin ◽

Sprague Dawley Rats ◽

Thickness Skin ◽

Improve Wound Healing

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Early wound repair versus later scar repair in children with treadmill hand friction burns

Journal of Burn Care & Research ◽

10.1093/jbcr/irab083 ◽

2021 ◽

Author(s):

Rong Zhou ◽

Lin Qiu ◽

Jun Xiao ◽

Xiaobo Mao ◽

Xingang Yuan

Keyword(s):

Skin Graft ◽

Wound Repair ◽

Full Thickness ◽

Full Thickness Skin Graft ◽

Vacuum Sealing ◽

Full Thickness Skin ◽

Thickness Skin ◽

Vacuum Sealing Drainage ◽

Repair Group ◽

Hand Friction

Abstract The incidence of pediatric treadmill hand friction burns has been increasing every year. The injuries are deeper than thermal hand burns, the optimal treatment remains unclear. This was a retrospective study of children who received surgery for treadmill hand friction burns from January 1, 2015, to December 31, 2019, in a single burn center. A total of 22 children were surveyed. The patients were naturally divided into two groups: the wound repair group (13 patients), which was admitted early to the hospital after injury and received debridement and vacuum sealing drainage initially, and a full-thickness skin graft later; and the scar repair group (9 patients), in which a scar contracture developed as a result of wound healing and received scar release and skin grafting later. The Modified Michigan Hand Questionnaire score in the wound repair group was 116.31 ± 10.55, and the corresponding score in the scar repair group was 117.56 ± 8.85 (P>0.05), no statistically significant difference. The Vancouver Scar Scale score in the wound repair group was 4.15 ± 1.21, and the corresponding score in the scar repair group was 7.22 ± 1.09 (P<0.05). Parents were satisfied with the postoperative appearance and function of the hand. None in the two groups required secondary surgery. If the burns are deep second degree, third degree, or infected, early debridement, vacuum sealing drainage initially, and a full-thickness skin graft can obviously relieve pediatric pain, shorten the course of the disease, and restore the function of the hand as soon as possible.

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