scholarly journals The Iinfluence TENS H Current Over Proliferation of Cancer Cells Depending on the Intensity in in vitro Assay

2019 ◽  
Vol 61 (3) ◽  
pp. 213-216
Author(s):  
Marzena Pełczyńska ◽  
Magdalena Milczarek ◽  
Magdalena Maciejewska ◽  
Joanna Wietrzyk
Keyword(s):  
Cancer Cells ◽  
In Vitro Assay ◽  
Cytotoxic Effects ◽  
Vitro Assay ◽  
Patients With Cancer ◽  
Neoplastic Process ◽  
Repeated Doses ◽  
Triple Dose ◽  
Mcf 7

Introduction: The knowledge of influence physiotherapeutic method over proliferation process is insufficient. It is considered that electrotherapeutic methods could accelerate neoplastic process. Thus using this methods are very limited in treatment patients with cancer anamnesis. Unfortunately, there are in literature not a lot of results of base researches in this area. It is much more difficult to exclude possibility proneoplastic activity of this methods than confirm this action. Aim: of the study is checking the influence of TENS current over proliferation of cancer cells in in vitro assay. Materials and Methods: The following human cell lines were used- A549, ES-2, HT29, MCF-7. The cells were plated twenty-four hours before treatment. Then the cells were exposured to TENS H current (0,1 mV and 1mV), t imp.100μs; f 100Hz, constant; during 20 min. The in vitro cytotoxic effects were examined after 96h in SRB assay. In the other experiment there were three- times expositions – 24, 48 and 72 hours after the plated. Results: In this assay it wasn’t observed the acceleration of proliferation of cancer cells after single dose or triple dose expositions to TENS H current. Conclusion: The TENS H current after repeated doses didn’t accelerate proliferation of cancer cells in in vitro conditions.

scholarly journals The influence of acupuncture TENS currents on the proliferation of cancer cells tested in vitro

2019 ◽  
Vol 23 (3) ◽  
Author(s):  
Marzena Pełczyńska ◽  
Magdalena Milczarek ◽  
Magdalena Maciejewska ◽  
Joanna Wietrzyk
Keyword(s):  
Cancer Cells ◽  
Cancer Cell Proliferation ◽  
Cytotoxic Effects ◽  
Electrical Nerve Stimulation ◽  
Patients With Cancer ◽  
Repeated Doses ◽  
Constant Duration ◽  
Triple Dose ◽  
Mcf 7

Introduction: Transcutaneous Electrical Nerve Stimulation (TENS) is a very popular, non-pharmacological antianalgetic method. Nonetheless, knowledge on using this method is very limited in the treatment of patients with cancer anamnesis. Unfortunately, there are not many results of research referring to the application of this method. It is much more difficult to exclude the possibility of proneoplastic activity regarding these methods than to confirm this action. Aim: The aim of the study was to evaluate the influence of TENS currents on the proliferation of cancer cells tested in vitro. Materials and methods: The following human cell lines were used - A549, ES-2, HT29, MCF-7. The cells were plated 24 h before treatment. Then, the cells were exposed to AL-TENS currents (0.1 mA, 1.0 mA and 10 mA), t imp. 200μs; f 2Hz, constant; duration: 20 min. The in vitro cytotoxic effects were examined after 96 h in SRB assay. In the other experiment, there were three expositions – 24, 48 and 72 hours from the beginning of the experiment. Results: In this assay, the acceleration of cancer cell proliferation after single or triple-dose expositions to AL-TENS currents was not observed. Conclusions: The AL-TENS current after repeated doses did not accelerate proliferation of cancer cells in in vitro conditions.

scholarly journals In Vivo and In Vitro Assay for Drug Effect on Cancer Cells

1963 ◽  
Vol 157 (5) ◽  
pp. 785-797 ◽  
Author(s):  
G. O. McDonald ◽  
A. N. Stroud ◽  
A. M. Brues ◽  
W. H. Cole
Keyword(s):  
Cancer Cells ◽  
Drug Effect ◽  
In Vitro Assay ◽  
Vitro Assay

scholarly journals Application of new ZnO nanoformulation and Ag/Fe/ZnO nanocomposites as water-based nanofluids to consider in vitro cytotoxic effects against MCF-7 breast cancer cells

2017 ◽  
Vol 45 (8) ◽  
pp. 1769-1777 ◽  
Author(s):  
Mohammad Hossein Abdolmohammadi ◽  
Faranak Fallahian ◽  
Zahra Fakhroueian ◽  
Mozhgan Kamalian ◽  
Peyman Keyhanvar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cytotoxic Effects ◽  
Water Based ◽  
Mcf 7

Aromatase (CYP19) inhibition by biflavonoids obtained from the branches of Garcinia gardneriana (Clusiaceae)

2019 ◽  
Vol 74 (9-10) ◽  
pp. 279-282 ◽  
Author(s):  
Angelica Maria Recalde-Gil ◽  
Luiz Klein-Júnior ◽  
Juliana Salton ◽  
Sérgio Bordignon ◽  
Valdir Cechinel-Filho ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Aromatase Inhibitors ◽  
Anticancer Agents ◽  
Breast Cancer Cells ◽  
In Vitro Assay ◽  
Aromatase Activity ◽  
Vitro Assay

Abstract Overexpression of aromatase in breast cancer cells may substantially influence its progression and maintenance. In this sense, the inhibition of aromatase is a key target for the treatment of breast cancer in postmenopausal women. Although several flavonoids had already demonstrated the capacity of inhibiting aromatase activity, the role of biflavonoids as aromatase inhibitors is poorly studied. In this work, the biflavonoids isolated from Garcinia gardneriana, morelloflavone (1), Gb-2a (2) and Gb-2a-7-O-glucose (3) were submitted to in vitro assay to evaluate the aromatase modulatory effect. As results, it was demonstrated that all biflavonoids were able to inhibit the enzyme, with IC50 values ranging from 1.35 to 7.67 μM. This demonstrates that biflavonoids are an important source of scaffolds for the development of new aromatase inhibitors, focusing on the development of new anticancer agents.

scholarly journals New Neo-Lignan From Acanthopanax senticosus and the Cytotoxic Effects on Human Cancer Cell Lines

2020 ◽  
Vol 15 (7) ◽  
pp. 1934578X2094129
Author(s):  
Zhi-Chao Feng ◽  
Sheng Wang ◽  
Jun Li ◽  
Jun-Sheng Wang
Keyword(s):  
Cancer Cells ◽  
Inhibitory Concentration ◽  
Human Cancer ◽  
In Vitro Cytotoxicity ◽  
Soluble Extract ◽  
Acanthopanax Senticosus ◽  
Cytotoxic Effects ◽  
Human Cancer Cell Lines ◽  
Mcf 7

A new neo-lignan named (7ʹ S,8ʹ R)-4ʹ,5ʹ,9ʹ-trihydroxy-5-methoxy-4,8ʹ-oxyneolign-7-en-9-al (1), together with 5 known compounds (2-6) were isolated from the ethylacetate-soluble extract of Acanthopanax senticosus. The structure of new neo-lignan was elucidated with spectroscopic and physicochemical analyses. All the isolates were evaluated for in vitro cytotoxicity against 4 human cancer lines including HepG2, A549, Hela, and MCF-7. Among them, compounds 1 and 3 showed the potent antiproliferative activities against the HepG2 cancer cells with half-maximal inhibitory concentration (IC50) values of 9.8 ± 1.6 and 15.0 ± 1.1 μM, respectively. In addition, compound 6 exhibited moderate inhibitory activity on MCF-7 cancer cells with an IC50 value of 18.3 ± 1.5 μM.

scholarly journals Induction of Apoptosis and Regulation of MicroRNA Expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) Treatment on MCF-7 Breast Cancer Cells

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1277
Author(s):  
Swee Keong Yeap ◽  
Norlaily Mohd Ali ◽  
Muhammad Nadeem Akhtar ◽  
Nursyamirah Abd Razak ◽  
Zhi Xiong Chong ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Quantitative Polymerase Chain Reaction ◽  
M Cell ◽  
Cytotoxic Effects ◽  
Er Positive ◽  
Mcf 7

(2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic effects of BHMC on ER positive breast cancer cells were not widely reported. This study was aimed to investigate the cytotoxic potential of BHMC on MCF-7 cells using cell viability, cell cycle, and apoptotic assays. Besides, microarray and quantitative polymerase chain reaction (qPCR) were performed to identify the list of miRNAs and genes, which could be dysregulated following BHMC treatment. The current study discovered that BHMC exhibits selective cytotoxic effects on ER positive MCF-7 cells as compared to ER negative MDA-MB-231 cells and normal breast cells, MCF-10A. BHMC was shown to promote G2/M cell cycle arrest and apoptosis in MCF-7 cells. Microarray and qPCR analysis demonstrated that BHMC treatment would upregulate several miRNAs like miR-3195 and miR-30a-3p and downregulate miRNAs such as miR-6813-5p and miR-6132 in MCF-7 cells. Besides, BHMC administration was also found to downregulate few tumor-promoting genes like VEGF and SNAIL in MCF-7. In conclusion, BHMC induced apoptosis in the MCF-7 cells by altering the expressions of apoptotic-regulating miRNAs and associated genes.

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