scholarly journals Effectiveness of crystal violet stain for localization of mitotic activity in oral squamous cell carcinoma

2020 ◽  
Vol 13 (4) ◽  
pp. 166-169
Author(s):  
Aneequa Sajjad ◽  
Muhammad Qasim Raza ◽  
Syeda Zaira Sajjad ◽  
Ihtesham-ud-Din Qureshi ◽  
Syed Sajjad Sarwar ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Crystal Violet ◽  
Cost Effective ◽  
Optical Microscope ◽  
Mitotic Figure ◽  
Cancer Pathology ◽  
Mitotic Figures

Background: Mitotic figure counting is simplest and oldest method for determining proliferative activity of cell. It is considered as one of the important diagnostic aid in cancer pathology. Though advanced methods to evaluate dysplastic features are more precise and definite but expense and time makes them less practicable for routine use. Therefore an effort was made to use economical as well as simple approach involving crystal violet stain (1%) to study the mitotic figures in oral squamous cell carcinoma. Materials and Methods: This descriptive research included samples, consisting of thirty three cases of the oral squamous cell carcinoma (OSCC). Representative sections were stained with H&E stain and 1% crystal violet stain respectively. The stained sections were viewed under optical microscope to count mitotic figures for evaluating the effectiveness of 1% crystal violet stain. Data obtained was statistically analyzed by using sample t-test. Results: There was noteworthy increase in the mean mitotic count among the crystal violet stained sections of OSCC in contrast to the OSCC sections stained with H&E (P = 0.00). Conclusion:1% Crystal violet stain can be considered as one of the optimum stains to observe the mitotic figure. Practice of staining with 1% crystal violet during routine histopathological procedures will be cost effective and may be used as a selective stain.

scholarly journals Evaluation of Mitotic Figures and Cellular and Nuclear Morphometry of Various Histopathological Grades of Oral Squamous Cell Carcinoma: Comparative study using crystal violet and Feulgen stains

2018 ◽  
Vol 18 (2) ◽  
pp. 149 ◽  
Author(s):  
Kashmira Kesarkar ◽  
Avinash Tamgadge ◽  
Treville Peirera ◽  
Sandhya Tamgadge ◽  
Swati Gotmare ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Crystal Violet ◽  
Image Cytometry ◽  
Control Group ◽  
Case Group ◽  
Selective Staining ◽  
Mitotic Figures

Objectives: The objectives of this study were to quantitatively estimate the number of mitotic figures (MFs) and evaluate the cellular and nuclear features of various histological grades of oral squamous cell carcinoma (OSCC) using Feulgen and 1% crystal violet stains. Methods: This case-control study took place at the Dr D. Y. Patil Dental College & Hospital in Mumbai, Maharashtra, India, between June and December 2016. A total of 51 samples were retrieved from the hospital archives. Of these, 15 well-differentiated, 15 moderately-differentiated and six poorly-differentiated OSCC samples formed the case group while 15 samples of normal gingival mucosa constituted the control group. Each sample was dyed using Feulgen and 1% crystal violet stains and the mitotic count, nuclear area (NA), cellular area (CA), nuclear perimeter (NP), cellular perimeter (CP) and nuclear-to-cytoplasmic (N/C) ratio was calculated using computeraided morphometry techniques. Results: The number of MFs visible per field was significantly higher in Feulgen-stained sections as compared to those stained with crystal violet (P = 0.050). In addition, the NA, NP, CA and CP values and N/C ratios of samples in the experimental group increased significantly in accordance with an increase in OSCC grade (P <0.001). Conclusion: The Feulgen stain is more reliable than 1% crystal violet in terms of the selective staining of MFs. Moreover, the findings of this study indicate that computer-based morphometric analysis is an effective tool for differentiating between various grades of OSCC.Keywords: Crystal Violet; Feulgen Stain; Mitotic Index; Image Cytometry; Squamous Cell Carcinoma; Oral Cancers.

Feulgen stain as a special stain for mitotic figures and apoptotic bodies in oral squamous cell carcinoma

2018 ◽  
Vol 9 (4) ◽  
pp. 164
Author(s):  
Sankari Radhakrishnan ◽  
Ramesh Venkatapathy ◽  
PD Balamurali ◽  
KarthikShree V Prashad ◽  
B Premalatha ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Apoptotic Bodies ◽  
Special Stain ◽  
Mitotic Figures

scholarly journals Correlation of mitosis obtained by using 1% crystal violet stain with Ki67LI in histological grades of oral squamous cell carcinoma

2018 ◽  
Vol 8 (3) ◽  
pp. 234-240
Author(s):  
Priyanka Kadoo ◽  
Rishikesh Dandekar ◽  
Meena Kulkarni ◽  
Aarti Mahajan ◽  
Ramniwas Kumawat ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Crystal Violet

scholarly journals USE OF CRYSTAL VIOLET STAIN FOR THE ASSESSMENT OF MITOTIC FIGURES IN ORAL EPITHELIAL DYSPLASIA AND ORAL SQUAMOUS CELL CARCINOMA

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Dr. Bipin Gandhi
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Crystal Violet ◽  
Control Group ◽  
Case Group ◽  
Apoptotic Bodies ◽  
Oral Epithelial Dysplasia ◽  
Mitotic Figures ◽  
Oral Epithelial

INTRODUCTION: Histopathological grading depends on several features, of which appearance of mitotic figures and apoptotic bodies in the tissue sections plays an important role. The mitotic figures and apoptotic bodies help assess the cellular proliferation and the turnover of the tumor. As mitotic figures (MF) and apoptotic bodies are difficult to assess under routine H and E sections, other methods such as immunohistochemistry (IHC) and flow cytometry have been implemented but, these methods are expensive and more time-consuming and are highly technique sensitive. Hence, stains, which should be cheap, and can be used routinely, and can be tried in identifying the mitotic figures and apoptotic bodies, which has come out with a better result. Routine histological procedures cannot differentiate between pyknotic nuclei, apoptotic cells and MFs which makes the diagnosis difficult. MATERIAL AND METHODS: Study samples were divided in to 3 groups, group 1- case group in which 40 samples of paraffin blocks diagnosed as oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC) and group 2- control group in which 40 samples from the healthy individuals. 5 micron thickness sections were made from each tissue specimen. One section was stained with H & E stain nd another section was stained with 1% crystal violet stain. RESULTS: In case group there were 24(60%) male and 16(40%) female while in control group there were 22 (55%) male and 18(45%) female. Mean age in case group was 36.2±15.4 and in control group was25.7±12.7. There is a significant increase in mitotic figures of H & E (P< 0.01) and crystal violet (P < 0.01). In case, there was a significant (P<0.05) increase in mitotic figures when stained with crystal violet. As compared to H&E stain there was a significant (P < 0.01) increase in metaphase observed in crystal violet stain. Mean mitotic figure by H&E in case group was 4.15±1.07 while in crystal violet it was 5.97±1.87. CONCLUSION: % crystal violet stain is the cheaper stain and can be performed in small laboratories and resource constraint settings. Crystal violet can be used for the localization of mitotic figures and assessing proliferation.

Touch Imprint Cytology: A Rapid and Accurate Method for Diagnosis of Oral Cancer

2019 ◽  
Vol 63 (5) ◽  
pp. 411-416
Author(s):  
Amit Kumar Adhya ◽  
Madhabananda Kar ◽  
Ranjan Mohanty
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Predictive Value ◽  
Cost Effective ◽  
Accurate Method ◽  
Imprint Cytology ◽  
Cytologic Examination ◽  
Touch Imprint Cytology

Objectives: Touch imprint cytology (TIC) is a rapid and cheap method and can be used as an adjunct to biopsy for the evaluation of suspected malignancy. We evaluated the advantages and limitations of TIC for the preoperative diagnosis of oral squamous cell carcinoma. Study Design: We used 280 incisional biopsies of clinically suspected oral squamous cell carcinoma and compared TIC diagnosis to histopathological sections. The sensitivity, specificity, and diagnostic accuracy of TIC were evaluated. Results: A total of 201 (82.5%) cases were diagnosed as malignant, 22 (7.9%) cases were diagnosed as suspicious of malignancy, and 54 (19.3%) cases as benign/negative on TIC. The overall sensitivity of TIC was 98.2%, specificity was 89.3%, the positive-predictive value 97.3%, and the negative-predictive value was 92.6%. The accuracy of the test was 96.4%. Conclusions: TIC is an accurate, simple, rapid, and cost-effective method that aids the diagnosis of oral squamous cell carcinoma but does not replace incisional biopsy. The cytologic examination of imprints of biopsies also helps determine the adequacy of the specimen for histologic examination.

scholarly journals Salivary Micro-RNA and Oral Squamous Cell Carcinoma: A Systematic Review

2021 ◽  
Vol 11 (2) ◽  
pp. 101
Author(s):  
Maria Menini ◽  
Emanuele De Giovanni ◽  
Francesco Bagnasco ◽  
Francesca Delucchi ◽  
Francesco Pera ◽  
...  
Keyword(s):  
Systematic Review ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cost Effective ◽  
Clinical Results ◽  
Micro Rna ◽  
Non Invasive ◽  
Cost Effective Method

Oral squamous cell carcinoma (OSCC) is a widespread malignancy with high mortality. In particular, a delay in its diagnosis dramatically decreases the survival rate. The aim of this systematic review was to investigate and summarize clinical results in the literature, regarding the potential use of salivary microRNAs (miRNAs) as diagnostic and prognostic biomarkers for OSCC patients. Twelve papers were selected, including both case–control and cohort studies, and all of them detected significantly dysregulated miRNAs in OSCC patients compared to healthy controls. Based on our results, salivary miRNAs might provide a non-invasive and cost-effective method in the diagnosis of OSCC, and also to monitor more easily its evolution and therapeutic response and therefore aid in the establishment of specific therapeutic strategies.

scholarly journals Assessment of Interobserver Variability in Mitotic Figure Counting in Different Histological Grades of Oral Squamous Cell Carcinoma

2012 ◽  
Vol 13 (3) ◽  
pp. 339-344 ◽  
Author(s):  
MD Prasanna ◽  
K Shailaja Yadav ◽  
Sudhir Gonuguntla ◽  
Surekha Reddy Velidandla ◽  
CR Charan Reddy ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Interobserver Variability ◽  
Mitotic Figure ◽  
Significant Difference ◽  
Group 2 ◽  
Mitotic Counting ◽  
Group 1

ABSTRACT Mitotic counting is often used for classification, grading and prognosis of tumors. The count usually stands as a decision point for treatment as well. The easiest way of counting the number of mitoses is done by screening routine H&E stained slides. However, for proper mitotic counting, certain strict protocols should be taken into consideration. This study on 30 cases of different grades of oral squamous cell carcinoma was undertaken to determine the interobserver variations in two different groups: Group1 (A1, A2), who were given certain criteria to be followed during the counting of the mitotic figures and group 2 investigators (B1, B2) who were unaware of such criteria. The paired t-test gives a correlation of 0.988 and a significant difference of 0.000 between the two investigators in group 1. The correlation was 0.650 with a significant difference of 0.058 between two investigators in-group 2, indicating that group 1 observers exhibit good interobserver agreement. The results emphasize that following of strict protocols are of great help in determining the accuracy of mitotic counting. How to cite this article Yadav KS, Gonuguntla S, Ealla KKR, Velidandla SR, Reddy CRC, Prasanna MD, Bommu SR. Assessment of Interobserver Variability in Mitotic Figure Counting in Different Histological Grades of Oral Squamous Cell Carcinoma. J Contemp Dent Pract 2012;13(3):339-344.

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