scholarly journals EXPRESSION OF ANTI-APOPTOTIC PROTEIN BCL-2 IN CUTANEOUS BASAL CELL CARCINOMA

2018 ◽  
Vol 4 (4) ◽  
Author(s):  
Vladimír Bartoš ◽  
Millada Kullová
Keyword(s):  
Basal Cell Carcinoma ◽  
Protein Expression ◽  
B Cell ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
High Expression ◽  
Biological Behavior ◽  
Positive Staining

Purpose: Overexpression of antiapoptotic B-cell lymphoma-2 (Bcl-2) protein is one of the major contributors to oncogenesis and high levels have been identified in a variety of tumour types. We investigated an immunohistochemicalexpression of Bcl-2 protein in cutaneous basal cell carcinomas (BCCs) to elucidate whether there are differences in the expression pattern related to tumour growth phenotype.Materials and Methods: The study group consisted of 45 cutaneous BCCs, which were categorised into the nonaggressive (NA-BCCs; 31 cases) and aggressive histologic variants (A-BCCs; 14 cases).Results: There were 3 tumours (6.6%) with negative staining and 42 tumours (93.4%) with positive staining for Bcl-2 protein, 10 of which (23.8%) displayed low and remaining 32 cases (76.2%) exhibited high expression. All three “Bcl-2 negative” BCCs showed aggressive-growth features (infiltrative subtypes). When Bcl-2 values were evaluated as negative/low versus high expression, there was significantly lower Bcl-2 protein expression in the A-BCCs comparedto the NA-BCCs. Even an intensity of immunostaining showed a tendency of being weaker in the A-BCCs. In spite of that, three infiltrative BCCs showed a diffuse strong immunoreactivity.Conclusion: An immunohistochemical positivity of Bcl-2 protein in the neoplastic cells of cutaneous BCC was nearly constant feature, and its decreased staining was associated with an infiltrative growth pattern. It suggests that a lowBcl-2 protein expression in tumor tissue might be considered an unfavorable prognostic indicator.Key words: Basal cell carcinoma, B-cell lymphoma-2 protein, biological behavior

74 DIFFUSE LARGE B-CELL LYMPHOMA MIMICKING ADVANCED BASAL CELL CARCINOMA.

2007 ◽  
Vol 55 (2) ◽  
pp. S360.5-S360
Author(s):  
E. Akinyemi ◽  
M. Le ◽  
P. Sircar ◽  
A. Maini ◽  
A. Barua ◽  
...  
Keyword(s):  
Basal Cell Carcinoma ◽  
B Cell ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Large B Cell Lymphoma ◽  
Large B Cell ◽  
Advanced Basal Cell Carcinoma

DIFFUSE LARGE B-CELL LYMPHOMA MIMICKING ADVANCED BASAL CELL CARCINOMA.

2007 ◽  
Vol 55 (2) ◽  
pp. S360
Author(s):  
E. Akinyemi ◽  
M. Le ◽  
P. Sircar ◽  
A. Maini ◽  
A. Barua ◽  
...  
Keyword(s):  
Basal Cell Carcinoma ◽  
B Cell ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Large B Cell Lymphoma ◽  
Large B Cell ◽  
Advanced Basal Cell Carcinoma

scholarly journals High Expression of Caspase-8 Associated with Improved Survival in Diffuse Large B-Cell Lymphoma: Machine Learning and Artificial Neural Networks Analyses

2021 ◽  
Vol 1 (1) ◽  
pp. 18-46
Author(s):  
Joaquim Carreras ◽  
Yara Yukie Kikuti ◽  
Giovanna Roncador ◽  
Masashi Miyaoka ◽  
Shinichiro Hiraiwa ◽  
...  
Keyword(s):  
Artificial Intelligence ◽  
Protein Expression ◽  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
High Protein ◽  
High Expression ◽  
Caspase 8 ◽  
Large B Cell Lymphoma ◽  
Large B Cell

High expression of the anti-apoptotic TNFAIP8 is associated with poor survival of the patients with diffuse large B-cell lymphoma (DLBCL), and one of the functions of TNFAIP8 is to inhibit the pro-apoptosis Caspase-8. We aimed to analyze the immunohistochemical expression of Caspase-8 (active subunit p18; CASP8) in a series of 97 cases of DLBCL from Tokai University Hospital, and to correlate with other Caspase-8 pathway-related markers, including cleaved Caspase-3, cleaved PARP, BCL2, TP53, MDM2, MYC, Ki67, E2F1, CDK6, MYB and LMO2. After digital image quantification, the correlation with several clinicopathological characteristics of the patients showed that high protein expression of Caspase-8 was associated with a favorable overall and progression-free survival (Hazard Risks = 0.3; p = 0.005 and 0.03, respectively). Caspase-8 also positively correlated with cCASP3, MDM2, E2F1, TNFAIP8, BCL2 and Ki67. Next, the Caspase-8 protein expression was modeled using predictive analytics, and a high overall predictive accuracy (>80%) was obtained with CHAID decision tree, Bayesian network, discriminant analysis, C5 tree, logistic regression, and Artificial Intelligence Neural Network methods (both Multilayer perceptron and Radial basis function); the most relevant markers were cCASP3, E2F1, TP53, cPARP, MDM2, BCL2 and TNFAIP8. Finally, the CASP8 gene expression was also successfully modeled in an independent DLBCL series of 414 cases from the Lymphoma/Leukemia Molecular Profiling Project (LLMPP). In conclusion, high protein expression of Caspase-8 is associated with a favorable prognosis of DLBCL. Predictive modeling is a feasible analytic strategy that results in a solution that can be understood (i.e., explainable artificial intelligence, “white-box” algorithms).

BCL2 protein expression parallels its mRNA level in normal and malignant B cells

Blood ◽  
2004 ◽  
Vol 104 (9) ◽  
pp. 2936-2939 ◽  
Author(s):  
Yulei Shen ◽  
Javeed Iqbal ◽  
James Z. Huang ◽  
Guimei Zhou ◽  
Wing C. Chan
Keyword(s):  
B Cells ◽  
Protein Expression ◽  
B Cell ◽  
Posttranscriptional Regulation ◽  
Cell Lymphoma ◽  
Dominant Role ◽  
Mrna Level ◽  
B Cell Lymphoma ◽  
Transcriptional Level ◽  
Bcl2 Protein

Abstract The regulation of B-cell lymphoma 2 (BCL2) protein expression in germinal center (GC) B cells has been controversial. Previous reports have indicated posttranscriptional regulation plays a dominant role. However, a number of recent studies contradicted these reports. Using real-time polymerase chain reaction (PCR) and Standardized Reverse Transcriptase-PCR (StaRT-PCR), we measured the level of mRNA expression in GC, mantle zone (MNZ), and marginal zone (MGZ) cells from laser capture microdissection. Both quantitative RT-PCR measurements of microdissected GC cells from tonsils showed that GC cells had low expression of BCL2 transcripts commensurate with the low protein expression level. These results are in agreement with microarray studies on fluorescence-activated cell sorter (FACS)-sorted cells and microdissected GC cells. We also examined BCL2 mRNA and protein expression on a series of 30 cases of diffuse large B-cell lymphoma (DLBCL) and found, in general, a good correlation. The results suggested that BCL2 protein expression is regulated at the transcriptional level in normal B cells and in the neoplastic cells in most B-cell lymphoproliferative disorders.

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