A rapid salting out method for DNA extraction from buccal swabs

2012 ◽  
Vol 31 (12) ◽  
pp. 1370-1374
Author(s):  
Wei-feng ZHU ◽  
Da-ya LUO ◽  
Shuo TU ◽  
Xia-li ZHANG ◽  
Ke-min JIE ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Salting Out ◽  
Buccal Swabs

scholarly journals Single Lysis-Salting Out Method of Genomic DNA Extraction From Dried Blood Spots

2016 ◽  
Vol 30 (6) ◽  
pp. 1009-1012 ◽  
Author(s):  
Muntaj Shaik ◽  
Devaraju Kuramkote Shivanna ◽  
Mahesh Kamate ◽  
Vedamurthy AB ◽  
Kruthika-Vinod TP
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Dried Blood Spots ◽  
Salting Out ◽  
Genomic Dna Extraction ◽  
Blood Spots

scholarly journals DNA Extraction from Hair Roots using Modified Salting out Method

2017 ◽  
Vol 8 (16) ◽  
pp. 145-151
Author(s):  
Mehdi Mokhber ◽  
Mostafa Sadeghi ◽  
Mohammad Moradi Shahrbabak ◽  
Hosein Moradi Shahr-Babak ◽  
Javad Rahmaninia ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Salting Out ◽  
Hair Roots

scholarly journals DNA Isolation: A Method for Improving the Efficiency of DNA Extraction From Clotted Blood Samples

2021 ◽  
Vol 1 (2) ◽  
pp. 44-47
Author(s):  
Bobby Rianto Adi Putra
Keyword(s):  
Dna Extraction ◽  
Ball Bearing ◽  
Dna Isolation ◽  
Genome Research ◽  
Salting Out ◽  
Blood Samples ◽  
Blood Clots ◽  
Safe Technique ◽  
Yield And Purity

In generally, the genome research of high DNA extraction from clotted blood produced a low quality. The aim of this study is to develop a simple and safe technique to dispering the blood clots by the ball bearing metal shots. The yield and purity of DNA obtained by three steps were significantly different (P < 0.0001), with a higher yield in the modified salting-out method. The salting-out method is simple, efficient and economical for DNA isolation from old clotted blood samples.

scholarly journals Improving DNA extraction quality by the salting‐out method to prepare blood samples for real‐time PCR. (LB124)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Natalia Araujo ◽  
Bruno Carmona ◽  
Glaucia Regina Nogueira ◽  
Marcor Ferreira Minicucci ◽  
Sandro Conde
Keyword(s):  
Real Time ◽  
Dna Extraction ◽  
Real Time Pcr ◽  
Salting Out ◽  
Blood Samples

scholarly journals Comparative account of DNA extraction protocols in some freshwater prawns of Genus Macrobrachium (Bate, 1868) (Family Palaemonidae) from Jammu waters for PCR based applications

2019 ◽  
Vol 12 (3) ◽  
pp. 1201-1206 ◽  
Author(s):  
Raman Jasrotia ◽  
Seema Langer
Keyword(s):  
Dna Extraction ◽  
Muscle Tissue ◽  
Genomic Dna ◽  
Uv Spectrophotometry ◽  
High Concentration ◽  
Salting Out ◽  
Basic Step ◽  
Tissue Samples ◽  
Inter Simple Sequence Repeats ◽  
High Yields

Genetic variations among prawns act as an important tool to characterize and differentiate between the species. Molecular and phylogenetic analysis of shrimps and prawns like any other organism rely on high yields of pure and better quality genomic DNA. In this regard isolation of DNA is the first and basic step. In spite of the availability of many protocols of DNA extraction from animal tissues, it is difficult to ascertain that which one would provide desired results for prawn tissue. In the present study, three different techniques of DNA isolation i.e., salting out, phenol-chloroform and Qiagen DNA extraction kit were performed and compared for their yield. Cephalothoracic tissue and muscle tissue of pleopods were used for isolation. Tissue samples from fresh specimens as well as from alcohol preserved specimens were employed for extraction. The quantity (µg/ml) and quality of isolated DNA were determined by UV spectrophotometry and agarose gel electrophoresis. Results showed that Phenol-chloroform method with slight modifications obtained higher yield of genomic DNA as compared to other methods. The present work also revealed that among fresh specimens cephalotoracic tissue yielded high concentration DNA than muscle tissue. However, among alcohol preserved specimens, the concentration of DNA was higher in muscle tissue of pleopods. The high quality DNA was then subjected to randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analysis. The DNAs produced clear, sharp and reproducible PCR (Polymerse chain reaction) product pattern.

scholarly journals ALLELE FREQUENCY, HETEROZIGOSITY, AND ALLELE MIGRATION IN JAVANESE AND MADURESE POPULATION IN MALANG AND MADURA, EAST JAVA INDONESIA

2017 ◽  
Vol 17 (1) ◽  
pp. 43
Author(s):  
Nikmatul Iza
Keyword(s):  
Allele Frequency ◽  
Dna Extraction ◽  
Pcr Amplification ◽  
Polyacrylamide Gel ◽  
Salting Out ◽  
Blood Samples ◽  
Band Profile ◽  
Program Software

FREKUENSI ALEL, HETEROZIGOSITAS DAN MIGRASI ALEL PADA POPULASI ETNIS JAWA DAN MADURA DI MALANG DAN MADURA,  JAWA TIMUR, INDONESA ABSTRAKPenelitian ini bertujuan untuk menentukan frekuensi alel, heterozigositas, dan migrasi alel pada populasi etnis Jawa dan Madura berdasarkan penanda 13 CODIS. Metode yang digunakan dalam penelitian ini adalah (1) Pengambilan sampel darah dari 5 populasi yang terdiri dari 3 populasi etnis jawa dan 2 populasi etnis Madura; (2) Isolasi DNA dari sampel darah dilakukan dengan menggunakan metode salting out; (3) Amplifikasi PCR dengan menggunakan 13 CODIS  yang terdiri dari TPOX, D3S1358, FGA, D5S818, CSF1PO, D7S820, D8S1179, TH01, VWA, D13S317, D16S539, D18S51, D21S11 dan divisualisasi dengan elektroforesis gel polyacrylamid 8%; serta (4) Profil pita dianalisis dengan program QuantityOne dan variasi pola pita DNA dianalisis dengan menggunakan program software GENEPOP package versi 4.2 yang akan digunakan untuk menentukan frekuensi alel, heterozigositas, dan migrasi alel. Hasil penelitian menunjukkan bahwa (1) Pada populasi etnis Jawa frekuensi alel yang memiliki keragaman tertinggi terdapat pada lokus D21S11 dengan jumlah alel sebanyak enam, lokus VWA dengan jumlah alel sebanyak lima, dan lokus FGA, TH01, D13S317, D16S539 dengan jumlah alel sebanyak empat yang digunakan sebagai penanda. Populasi etnis Madura memiliki frekuensi alel dengan keragaman tertinggi terdapat pada lokus TH01, D13S317, dan D21S11 dengan jumlah alel sebanyak lima dan lokus FGA dengan jumlah alel sebanyak empat. (2) Nilai heterozigositas populasi etnis Madura I (90.38%) dan populasi etnis Madura II (86.54%) lebih tinggi dibandingkan dengan populasi etnis Jawa I (67.69%), populasi etnis Jawa II (83.03%), maupun populasi etnis Jawa III (70.77%) dan (3) Migrasi alel pada populasi etnis Jawa sebesar 0.085% dan pada populasi etnis Madura sebesar 0.081%.Kata kunci: Frekuensi Alel, Heterozigositas, Migrasi Alel, 13 CODIS, Etnis Jawa dan Madura ALLELE FREQUENCY, HETEROZIGOSITY, AND ALLELE MIGRATION IN JAVANESE AND MADURESE POPULATION IN MALANG AND MADURA, EAST JAVA INDONESIA ABSTRACTThe aim of this study is to determine the frequency of alleles, heterozygosity, and allele migration in Javanese and Madurese population use 13 CODIS. The methods used in this study were (1) blood samples from five population consist three population of Javanese ethnic and two population of Madurese ethnic, (2) DNA extraction from blood samples by salting out method, (3) PCR amplification use 13 CODIS were TPOX, D3S1358, FGA, D5S818, CSF1PO, D7S820, D8S1179, TH01, VWA, D13S317, D16S539, D18S51, D21S11 and visualized by 8% polyacrylamide gel (4) The band profile was analyzed by using QuantityOne software and variations in the pattern of DNA bands were analyzed by using GENEPOP software version 4.2 package to determine the frequency of alleles, heterozygosity, and allele migration. The results showed that (1) The population of Javanese allele frequency that has the highest diversity found in the locus D21S11 with a number of alleles of six, locus VWA by the number of alleles of five, and the locus FGA, TH01, D13S317, and D16S539 with a number of alleles of four  which is used as a marker. The populations of Madurese allele frequency that has the highest diversity found in the locus TH01, D13S317, and D21S11 with a number of alleles of five and locus FGA with a number of alleles of four, (2) Value of heterozygosity populations of Madurese I (90.38 %) and populations of Madurese II (86.54%) was higher than the population of Javanese I (67.69%), the population of Javanese II (83.03%), as well as the population of Javanese III (70.77%) and (3) There has been a migration of alleles in population Javanese of 0.085% and population Madurese of 0.081%.Keywords: Allele Frequency, Heterozigosity, Allele Migration, 13 CODIS,       Javanese and Madurese ethnic.

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