scholarly journals Changes in Mass and Performance in Rabbit Muscles after Muscle Damage with or without Transplantation of Primary Satellite Cells

2002 ◽  
Vol 11 (2) ◽  
pp. 169-180 ◽  
Author(s):  
Ramzi Boubaker El Andalousi ◽  
Paul-André Daussin ◽  
Jean-Paul Micallef ◽  
Colette Roux ◽  
Jean Nougues ◽  
...  
Keyword(s):  
Muscle Damage ◽  
Satellite Cells ◽  
And Performance

scholarly journals Skeletal muscle regeneration is altered in the R6/2 mouse model of Huntington's disease

2022 ◽  
Author(s):  
Sanzana Hoque ◽  
Marie Sjogren ◽  
Valerie Allamand ◽  
Kinga Gawlik ◽  
Naomi Franke ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Muscle Damage ◽  
Satellite Cell ◽  
Mouse Models ◽  
Satellite Cells ◽  
Muscle Fibers ◽  
Cag Repeat ◽  
Skeletal Muscle Regeneration

Huntington's disease (HD) is caused by CAG repeat expansion in the huntingtin (HTT) gene. Skeletal muscle wasting alongside central pathology is a well-recognized phenomenon seen in patients with HD and HD mouse models. HD muscle atrophy progresses with disease and affects prognosis and quality of life. Satellite cells, progenitors of mature skeletal muscle fibers, are essential for proliferation, differentiation, and repair of muscle tissue in response to muscle injury or exercise. In this study, we aim to investigate the effect of mutant HTT on the differentiation and regeneration capacity of HD muscle by employing in vitro mononuclear skeletal muscle cell isolation and in vivo acute muscle damage model in R6/2 mice. We found that, similar to R6/2 adult mice, neonatal R6/2 mice also exhibit a significant reduction in myofiber width and morphological changes in gastrocnemius and soleus muscles compared to WT mice. Cardiotoxin (CTX)-induced acute muscle damage in R6/2 and WT mice showed that the Pax7+ satellite cell pool was dampened in R6/2 mice at 4 weeks post-injection, and R6/2 mice exhibited an altered inflammatory profile in response to acute damage. Our results suggest that, in addition to the mutant HTT degenerative effects in mature muscle fibers, expression of mutant HTT in satellite cells might alter developmental and regenerative processes to contribute to the progressive muscle mass loss in HD. Taken together, the results presented here encourage further studies evaluating the underlying mechanisms of satellite cell dysfunction in HD mouse models.

scholarly journals Effects of nandrolone decanoate on the viability of muscle satellite cells during the differentiation process

2014 ◽  
Vol 21 (1) ◽  
pp. 16-20
Author(s):  
Beatriz Guimaraes Ribeiro ◽  
Kristianne Porta Santos Fernandes ◽  
Mikaele Tavares Silva ◽  
Simone Oliveira Sierra ◽  
Sandra Kalil Bussadori ◽  
...  
Keyword(s):  
Satellite Cells ◽  
Anabolic Steroids ◽  
Horse Serum ◽  
Muscle Cells ◽  
C2c12 Cells ◽  
Nandrolone Decanoate ◽  
Significance Level ◽  
Mtt Method ◽  
And Performance ◽  
Cycle Regulation

Studies indicate that the anabolic nandrolone decanoate (Deca-Durabolin(r)) can modulate cell cycle regulation, but little is known about its effects on muscle cells. Anabolic steroids are used, especially by athletes, to improve muscle mass and performance in the practice of exercises. The aim of this study was to evaluate the effect of the anabolic Deca-Durabolin(r) on the proliferation of skeletal muscle precursor cells C2C12. Cells were grown in Dulbecco's Modified Eagle Medium (DMEM), being supplemented with 10% Fetal Bovine Serum (FBS) and subjected to differentiation by the addition of 2% horse serum. They were incubated with anabolic at concentrations of 5, 10, 25 and 50 µM. The groups that received no anabolic or vehicle served as controls. The viability (proliferation) was evaluated by the MTT method (3-[4,5-Dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide; Thiazolyl blue) after one, three and five days of incubation. Three independent experiments were performed in each of the mentioned conditions, and the results were submitted to statistical analysis with significance level of p≤0.05 (ANOVA/Dunnett). Results showed no difference in viability between muscle cells treated with anabolic and the control cultures in all parameters. In conclusion, nandrolone, at the used concentrations, was not able to alter the viability of muscle C2C12 satellite cells.

scholarly journals Effects of Zembrin® (Sceletium tortuosum) Supplementation on Mood, Soreness, and Performance Following Unaccustomed Resistance Exercise: A Pilot Study

2021 ◽  
Vol 1 (1) ◽  
pp. 2-11
Author(s):  
Angela Berry ◽  
Haley Langley ◽  
Rebecca Rogers ◽  
Courteney Benjamin ◽  
Tyler Williams ◽  
...  
Keyword(s):  
Resistance Exercise ◽  
Muscle Damage ◽  
Exercise Performance ◽  
Muscle Soreness ◽  
Perceived Exertion ◽  
Mood State ◽  
Day Treatment ◽  
Short Term ◽  
And Performance

The purpose of this study was to investigate acute Zembrin® (Sceletium tortuosum) supplementation on muscle soreness, markers of muscle damage, mood, and exercise performance following unaccustomed resistance exercise. Untrained females (n = 16) were divided into two groups with a different three-day treatment regimen: (1) placebo (PL) and (2) Zembrin® (ZEM). During the initial visit, baseline perceived soreness, range of motion (ROM), mood state (profile of mood states (POMS) questionnaire), and plasma lactate dehydrogenase concentrations (LDH) were measured followed by the performance of an eccentric bicep curl protocol with their non-dominant arm. The total repetitions and rate of perceived exertion (RPE) were recorded throughout the exercise. The participants then supplemented with the corresponding treatment immediately following, the subsequent day, and 30 min prior to completing a 48 h follow-up visit. For the 48 h visit, all procedures were repeated and comparisons were drawn for perceived soreness, ROM, LDH, mood scores, total repetitions, and RPE. The findings indicate that short-term ZEM supplementation resulted in lower perceived soreness (p = 0.020) and a greater preservation of ROM (p = 0.028) at 48 h versus the PL group. Mood worsened from the baseline to 48 h regardless of the treatment (p = 0.043) but the decrements were exacerbated in the PL group compared with the ZEM group (p < 0.001). LDH levels (p = 0.019) and RPE (p = 0.008) were higher and total repetitions were lower (p < 0.001) at 48 h irrespective of the treatment. Although short-term dietary enrichment with ZEM did not alter the exercise performance or biomarkers of muscle damage, the current results suggest ZEM supplementation may be effective in reducing the markers of soreness and preserve mood following unaccustomed eccentric exercise.

The effect of time of day and recovery type after a football game on muscle damage and performance in anaerobic tests on young soccer players

2016 ◽  
Vol 47 (5) ◽  
pp. 797-814 ◽  
Author(s):  
Intissar Shimi ◽  
Salma Abedelmalek ◽  
Khouloud Aloui ◽  
Hamdi Chtourou ◽  
Nizar Souissi
Keyword(s):  
Muscle Damage ◽  
Time Of Day ◽  
Soccer Players ◽  
Football Game ◽  
And Performance

scholarly journals Time Course of Recovery for Performance Attributes and Circulating Markers of Muscle Damage Following a Rugby Union Match in Amateur Athletes

Sports ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 64 ◽  
Author(s):  
Bruno Victor Corrêa da Silva ◽  
Mário Antônio de Moura Simim ◽  
Rodrigo Barboza da Silva ◽  
Edmar Lacerda Mendes ◽  
Bernardo Neme Ide ◽  
...  
Keyword(s):  
Muscle Damage ◽  
Time Course ◽  
Rugby Union ◽  
Test Time ◽  
Amateur Athletes ◽  
Change Of Direction ◽  
Circulating Markers ◽  
Time Courses ◽  
And Performance ◽  
Run Test

Background: We sought to determine the time course of changes in neuromuscular performance and muscle damage following a single rugby union match. Methods: Fourteen male amateur rugby players (28.9 ± 3.5 yrs; 1.7 ± 5.1 m; 86.1 ± 11.1 kg) participated. Plasma activity of creatine kinase ([CK]) and lactate dehydrogenase (LDH), L-run test (change of direction) and 30-m sprint (T30; speed) with 10-m lap time (T10; acceleration) were assessed on six occasions: one week before the match (PRE) and immediately, 24, 48, 72, and 96 h post-match. Results: Relative to PRE, LDH was elevated immediately post-match (+33.6% ± 13.6%; p < 0.001) and [CK] was elevated immediately (+64.1% ± 38.8%, p = 0.001) and 24 h post-match (+352% ± 317%; p = 0.024). L-run test time increased 16.0 ± 8.7% relative to PRE at 24 h post (p < 0.001) and remained elevated through 96 h post-match (p < 0.05). T10 and T30 times increased relative to PRE immediately post-match (+12.0% ± 10.4%, p = 0.008; and +6.1% ± 4.9%; p = 0.006, respectively), though T30 times were similar to baseline by 48 h post-match whereas T10 times remained elevated through 72 h post-match. Conclusions: A single, competitive rugby union match induces significant muscle damage and performance decrements with distinct time courses of recovery in amateur athletes. Notably, change of direction attributes (i.e., L-run) appear to have the longest time course to full recovery.

scholarly journals The Effects of Spirulina Supplementation on Redox Status and Performance Following a Muscle Damaging Protocol

2021 ◽  
Vol 22 (7) ◽  
pp. 3559
Author(s):  
Aggelos Pappas ◽  
Athanasios Tsiokanos ◽  
Ioannis Fatouros ◽  
Athanasios Poulios ◽  
Dimitris Kouretas ◽  
...  
Keyword(s):  
Muscle Damage ◽  
Muscle Soreness ◽  
Peak Torque ◽  
Redox Status ◽  
Muscle Performance ◽  
Protein Carbonyls ◽  
Post Exercise ◽  
Beneficial Effects ◽  
Total Antioxidant ◽  
And Performance

Spirulina plantensis is a popular supplement which has been shown to have antioxidant and performance enhancing properties. The purpose of this study was to evaluate the effects of spirulina supplementation on (a) redox status (b) muscle performance and (c) muscle damage following an eccentric bout of exercise that would induce muscle damage. Twenty-four healthy, recreationally trained males participated in the study and were randomly separated into two groups: a spirulina supplementation (6 g per day) and a placebo group. Both groups performed an eccentric bout of exercise consisting of 5 sets and 15 maximum reps per set. Blood was collected at 24, 48, 72 and 96 h after the bout and total antioxidant capacity (TAC) and protein carbonyls (PC) were assessed in plasma. Delayed onset muscle soreness (DOMS) was also assessed at the same aforementioned time points. Eccentric peak torque (EPT) was evaluated immediately after exercise, as well as at 24, 48, 72 and 96 h post exercise. Redox status indices (TAC and PC) did not change significantly at any time point post exercise. DOMS increased significantly 24 h post exercise and remained elevated until 72 h and 96 h post exercise for the placebo and spirulina group, respectively. EPT decreased significantly and immediately post exercise and remained significantly lower compared to baseline until 72 h post exercise. No significant differences between groups were found for DOMS and EPT. These results indicate that spirulina supplementation following a muscle damaging protocol does not confer beneficial effects on redox status, muscle performance or damage.

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