Spectrophotometric Determination of Copper (II) with 5-Hydroxy-6-Mercapto-Benzo [a] Phenazine

2008 ◽  
Vol 59 (1) ◽  
pp. 3-7
Author(s):  
Aurora Reiss ◽  
Mihaela Mureseanu ◽  
Nicolae Muresan
Keyword(s):  
Spectrophotometric Determination ◽  
Molar Absorptivity ◽  
Molar Ratio ◽  
Variation Method ◽  
Ratio Method ◽  
Relative Standard ◽  
Analytical Reagent ◽  
Experimental Values ◽  
Determination Of Copper

5-Hydroxy-6-mercapto-benzo [a] phenazine (HMBP) is proposed as a sensitive and selective analytical reagent for the extractive spectrophotometric determination of copper (II). Copper reacts with HMBP to give a dark red complex which is soluble in chloroform. The composition of the CuII � HMBP complex is established as 1:2 by Job�s continuous variation method and molar ratio method. The instability constant of the complex calculated by Job�s relation for nonisomolar series is 4.65 . 10-9, at room temperature. The CuII-HMBP complex in chlorophorm shows a maximum absorbance at 495 nm, with molar absorptivity and Sandell�s sensitivity values of 7.39 . 103dm3 mol-1 cm-1 and 0.012 mg cm-2, respectively. Beer�s law is obeyed in the concentration range 0.67 - 26.90 mg mL-1 and the detection limit is 0.59 mg mL-1. A repetition of the method is checked by finding the relative standard deviation (RSD) at 1.00 mg mL-1 CuII of 0.6%. The method is successfully employed for the determination of copper (II) in environmental samples. The reliability of the method is assured by analysing the standard alloys and by inter-comparison of experimental values, using an atomic absorption spectrometer.

scholarly journals Spectrophotometric Determination of Selenium in Various Samples Using 5,6-Diaminouracil Hydrochloride (DAUH)

2015 ◽  
Vol 61 ◽  
pp. 128-136 ◽  
Author(s):  
Salwa Fares Rassi
Keyword(s):  
Acidic Medium ◽  
Metal Ion ◽  
Molar Absorptivity ◽  
Molar Ratio ◽  
Ratio Method ◽  
Colored Complex ◽  
Molar Ratio Method ◽  
Analytical Reagent ◽  
Color Reactions

A simple, rapid and sensitive spectrophotometric method was developed for the determination of Se (IV) using 5,6-diaminouracil hydrochloride (DAUH) as an analytical reagent. The reagent has been synthesized and characterized using IR, 1H NMR. The metal ion in aqueous medium forms yellow colored complex with DAUH showing maximum absorbance at 343 nm. Hence, analytical studies were further carried out at 343 nm. The reagent reacts with selenium in acidic medium to form yellow colored 1:1 (M:L) complex . The color reactions are instantaneous and absorbance values remain constant for one week. The composition of the Se (IV) complex with DAUH was studied by the method of job’s continuous variation and molar ratio method. Beer’s law was obeyed in the range 0.2-15.0 μg mL−1of Se (IV). The molar absorptivity and Sandell’s sensitivity of the method were found to be 0.5×10+4 L.mol-1.cm-1and 0.031μg.cm-2 respectively. Since DAUH method is more sensitive, it was applied for the determination of selenium in samples.

Solvent Extraction and Spectrophotometric Determination of Uranium (VI) Using 2,2′-[1,2-phenylenebis(nitrilomethylidene)]bisphenol (BSOPD) as an Analytical Reagent

2013 ◽  
Vol 12 ◽  
pp. 125-133 ◽  
Author(s):  
Sebastian Satya ◽  
Pravin U. Singare ◽  
R.S. Lokhande
Keyword(s):  
Molar Absorptivity ◽  
Variation Method ◽  
Ratio Method ◽  
Slope Ratio ◽  
Masking Agents ◽  
Mole Ratio Method ◽  
Intense Absorption ◽  
Analytical Reagent ◽  
Slope Ratio Method

2,2'-[1,2-phenylenebis(nitrilomethylidene)]bisphenol (BSOPD) is synthesized in the laboratoryand characterized by NMR, IR and elemental analysis for its purity. This reagent forms a yellowcomplex with U(VI) which can be quantitatively extracted into chloroform at pH 10. This complex inchloroform shows an intense absorption peak at 413.0 nm. It is observed that Beer’s law is obeyed inthe range of 2.0-10.0 ppm of metal solution. It gives a linear and reproducible graph under appropriateconditions, the complex having a molar absorptivity of 3.69 x 104 L mol-1·cm-1. Sandell’s sensitivitycalculated was found to be 4.4 x 10-3μg·cm-2. Nature of the extracted complex, determined by Job’scontinuous variation method, Slope ratio method and Mole ratio method shows that the compositionof U(VI) : BSOPD complex is 1:4. Interference by various ions is studied and masking agents usedwhere required. The proposed method has been applied for the determination of U(VI) in monazite sand and synthetic samples. The results of the analysis are found to be comparable with those obtained by standard method.

scholarly journals A Double-Line Sequential Injection System for the Spectrophotometric Determination of Copper, Iron, Manganese, and Zinc in Waters

2005 ◽  
Vol 88 (2) ◽  
pp. 639-644 ◽  
Author(s):  
Inês P A Morais ◽  
M Renata S Souto ◽  
António O S S Rangel
Keyword(s):  
Metal Ions ◽  
Spectrophotometric Determination ◽  
Absorption Spectrometry ◽  
Injection System ◽  
Sequential Injection ◽  
Double Line ◽  
Relative Standard ◽  
Iron Manganese ◽  
Determination Of Copper

Abstract A double-line sequential injection system was developed for the spectrophotometric determination of several metal ions in waters. The proposed double-line configuration was used to enable adding sample and chromogenic reagents as merging zones. The methodology was applied to the spectrophotometric determination of copper, iron, manganese, and zinc in samples of diverse origins at the range of 0.15–5.00, 0.10–10.0, 0.48–4.00, and 0.11–5.00 mg/L, respectively. Different chromogenic reagents and detection wavelengths were used. The chromogenic reagents for iron and manganese were 1,10-phenanthroline and formaldoxime, respectively. Copper and zinc were both determined using the analytical reagent zincon. Analytical characteristics of the methodology, such as manifold parameters, buffer pH, and reagent concentrations were optimized, and interference of some of the metal ions commonly present in water sample was assessed. Results of the analysis were in agreement with those obtained by atomic absorption spectrometry. Repeatability, expressed as the relative standard deviation for 10 consecutive injections of water samples, was lower than 6%. The determination rate was approximately 36/h.

scholarly journals 2-Hydroxy-5-methylbenzophenone oxime(HMBO) as an Analytical Reagent for Gravimetric Determination of Cu(II)

2007 ◽  
Vol 4 (1) ◽  
pp. 97-102 ◽  
Author(s):  
S. K. Shingadia ◽  
K. K. Desai
Keyword(s):  
Copper Ion ◽  
Molar Absorptivity ◽  
Ratio Method ◽  
Formation Reaction ◽  
Gravimetric Determination ◽  
Analytical Reagent ◽  
Gibb’S Free Energy ◽  
The Stability ◽  
Job's Method

2-Hydroxy-5-methylbenzophenone oxime (HMBO) was developed as a new analytical reagent for the gravimetric determination of divalent copper ion. In pH rang of 3.0 to 6.0, the reagent gives a buff colored precipitate with Cu(II). Job’s method and mole ratio method revealed that the stoichiometry of the complex is 1:2 (metal: ligand). Beer’s law is obeyed up to 61.25 ppm of Cu(II). Molar absorptivity and Sandell’s sensitivity at 400 nm were found to be 6.32 × 102L mol-1cm-1and 0.359 μg/cm2respectively. The stability constant of Cu(II)-HMBO complex is found to be 2.43 × 109. Gibb’s free energy change for complex formation reaction was found to be -12.88 Kcal/mol. The reagent can be used for the analysis of brass and alloy.

Spectrophotometric Determination of Boron in Dates of Some Cultivars Grown in Saudi Arabia

1993 ◽  
Vol 76 (3) ◽  
pp. 601-603 ◽  
Author(s):  
A A Al-Warthan ◽  
S S Al-Showiman ◽  
S A Al-Tamrah ◽  
A A BaOsman
Keyword(s):  
Saudi Arabia ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

Abstract The formation of a red complex between boron and the quinalizarin reagent was investigated and used as the basis for a simple and sensitive spectrophotometric method for boron in date cultivars. At 620 nm, the absorbance was linear (r= 0.999) over the 0.25-2.5 μg/mL concentration range. The molar absorptivity was found to be 2.23 x 103mol-1cm-1 and the relative standard deviation for 10 replicates (1.0 μg/mL) was 0.97%.

Hydroxyamidines as New Extracting Reagents for Spectrophotometric Determination of Cadmium with 4-(2-Pyridylazo)naphthol in Industrial Effluents, Coal, and Fly Ash

1993 ◽  
Vol 76 (3) ◽  
pp. 604-608 ◽  
Author(s):  
Sumon Chakravarty ◽  
Manas Kanti Deb ◽  
Rajendra Kumar Mishra
Keyword(s):  
Spectrophotometric Determination ◽  
Environmental Samples ◽  
Present Method ◽  
Industrial Effluents ◽  
Molar Absorptivity ◽  
Binary Complex ◽  
Matrix Interference ◽  
Relative Standard ◽  
The Common

Abstract A simple, sensitive, and selective extractive spectrophotometric method for the determination of cadmium in trace quantities with N1-hydroxy-N1,N2- diphenylbenzamidine (HDPBA) and 4-(2-pyridylazo) naphthol (PAN) is described. The method is based on the extraction of cadmium with HDPBA into chloroform at pH 9.0 ± 0.2 and simultaneous spectrophotometric determination wiith PAN. The binary Cd(ll)-HDPBA complex extracted into chloroform has a molar absorptivity of 1.96 x 104L/ mol/cm at λmax 400 nm. The sensitivity of the yellow Cd(ll)-HDPBA complex was increased remarkably by the addition of PAN to the binary complex. With 6 different hydroxyamidines tested, the red-orange complex in chloroform exhibited maximum absorbance at 530-550 nm, with molar absorptivity values of 3.2-5.6 x 104L/mol/cm. The method adheres to Beer’s law up to 1.5 μg cadmium/mL organic phase. The detection limit of the method is 0.02 μg Cd/mL. Investigations of the effect of foreign ions revealed that the present method is free from matrix interference of most of the common ions (e.g., Fe(lll), Ni(ll), Cu(ll), Mn(ll), V(V), Co(ll), Al(lll), Cu(ll), Mg(ll), and Mo(VI)). The relative standard deviation for 10 repetitive analyses of the metal was 1.4%. The validity of the method was tested successfully with various environmental samples.

Use of Acidic Hydrolysis and Diazo Coupling Reaction for Spectrophotometric Determination of Furosemide in Urine and Pharmaceutical Formulation.

2018 ◽  
pp. 225
Author(s):  
Hawraa Ali ◽  
Sumayha Muhammed
Keyword(s):  
Spectrophotometric Determination ◽  
Limit Of Detection ◽  
Coupling Reaction ◽  
Molar Absorptivity ◽  
Pharmaceutical Formulation ◽  
Basic Medium ◽  
Ratio Method ◽  
Diazo Coupling ◽  
Limit Of Quantitation

     Simple and rapid spectrophotometric determination of furosemide (FUR) has been investigated .The method is based on acid hydrolysis of FUR to free primary aromatic amine and diazotization followed by coupling with 3, 5 di methyl phenol (3, 5-DMPH) at basic medium. The absorbance was measured at 434 nm, the method was optimized for best condition, and beers’ law is obeyed over the range of 0.4-50 µg.mL-1 with molar absorptivity and sandal’s sensitivity 1.3899 x104 L moL-1 .cm-1 and 0.0238x104 µg.cm-2 respectively. Analysis of solution containing nineteen different concentrations of FUR gave a correlation coefficient of (0.9999) and limit of detection, limit of quantitation were 0.127, 0.464µg.mL-1 respectively. The reaction stoichiometry was evaluated by Job’s and mole ratio method was found to be 1:1(diazotized FUR: 3, 5-DMPH) .The method was applied in synthetic urine and pharmaceutical formulation. The recovery of FUR in spiked urine was satisfactory resulting in the values of (99±3.32) %, the results of the suggested method was compared with available official literature method.

Flotation-Dissolution-Spectrophotometric Determination of Phorate in Various Environmental Samples

2020 ◽  
Vol 33 (1) ◽  
pp. 18-23
Author(s):  
Harshita Sharma ◽  
Anushree Saha ◽  
Chhaya Bhatt ◽  
Kalpana Wani ◽  
Ajay Kumar Sahu ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Determination ◽  
Acidic Medium ◽  
Molar Absorptivity ◽  
Ammonium Molybdate ◽  
Organophosphate Pesticide ◽  
Organic Layers ◽  
Relative Standard ◽  
Ion Associate

The proposed method is based on flotation–dissolution an easy, impressible, extractive spectrophotometric determination, explained for easy investigation of the organophosphate pesticide phorate (O,O-diethyl S-[ethylthiomethyl] phosphorodithioate) on trace levels. A molybdophospho complex is generated when prorate is treated with ammonium molybdate in acidic medium. As an ion associate complex with methylene blue the complex is present in between of the water and organic layers which is extracted and then dissolved with acetone. The greenish blue complex produced show absorption maxima at 660 nm. Beer’s law range is found to be 0.5 to 16 µg per 10 ml for phorate. The molar absorptivity is 0.989×103 L mol-1 cm-1 and sandell’s sensitivity is 1.00×10-5 µg cm-2. Also calculated the standard deviation and relative standard deviation for the above method were ±0.006 and 1.95% respectively. The method has been applied and checked for the determination of phorate in water, soil and vegetables.

scholarly journals 2-Hydroxy-4-n-propoxy-5-bromoacetophenone oxime as an Analytical Reagent for Gravimetric Determination of V(V)

2009 ◽  
Vol 6 (2) ◽  
pp. 303-307 ◽  
Author(s):  
Ambily P. Nair ◽  
J. Christine
Keyword(s):  
Molar Absorptivity ◽  
Ratio Method ◽  
Gravimetric Determination ◽  
Pentavalent Vanadium ◽  
Analytical Reagent ◽  
Gibb’S Free Energy ◽  
The Stability ◽  
Ph Range ◽  
Job's Method

2-Hydroxy-4-n-propoxy-5-bromoacetophenone oxime (HnPBAO) was developed as a new analytical reagent for the gravimetric determination of pentavalent vanadium ion. In the pH range 4.0-6.0, the reagent gave a brown coloured precipitate with V(V). Job’s method and Mole ratio method revealed that the stoichiometry of the complex is 1:1 (metal: ligand). Beer’s law is obeyed up to 20.38 ppm of V(V). Molar absorptivity and Sandells sensitivity at 450 nm were found to be 10.22 × 102L/mol/cm and 0.049 µg/cm2respectively. The stability constant of V(V)-HnPBAO complex is found to be 1.195 × 106. Gibb’s free energy change for complex formation reaction was found to be –8.34 kcal/mol. The reagent can be used for the analysis of vanadium in ferro-vanadium alloys.

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