Isolation of a Tryptophan-Overproducing Strain Generated by EMS Mutagenesis of Candida rugosa

Abstract We identified and characterized the Drosophila gene ear (ENL/AF9-related), which is closely related to mammalian genes that have been implicated in the onset of acute lymphoblastic and myelogenous leukemias when their products are fused as chimeras with those of human HRX, a homolog of Drosophila trithorax. The ear gene product is present in all early embryonic cells, but becomes restricted to specific tissues in late embryogenesis. We mapped the ear gene to cytological region 88E11-13, near easter, and showed that it is deleted by Df(3R)ea5022rx1, a small, cytologically invisible deletion. Annotation of the completed Drosophila genome sequence suggests that this region might contain as many as 26 genes, most of which, including ear, are not represented by mutant alleles. We carried out a large-scale noncom-plementation screen using Df(3R)ea5022rx1 and chemical (EMS) mutagenesis from which we identified sevenc novel multi-allele recessive lethal complementation groups in this region. An overlapping deficiency, Df(3R)Po4, allowed us to map several of these groups to either the proximal or the distal regions of Df(3R)ea5022rx1. One of these complementation groups likely corresponds to the ear gene as judged by map location, terminal phenotype, and reduction of EAR protein levels.

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Candida rugosa Lipase-Mediated Enantioselective Acetylation Studies on (.+-.)-3-Arylmethyl-3-hydroxymethyl-2,3-dihydro-1-benzopyran-4(H)-ones.

ChemInform ◽

10.1002/chin.200523030 ◽

2005 ◽

Vol 36 (23) ◽

Author(s):

Smriti Trikha ◽

Rajesh Kumar ◽

Ashish Dhawan ◽

Poonam Poonam ◽

Ashok K. Prasad ◽

...

Keyword(s):

Candida Rugosa ◽

Candida Rugosa Lipase

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Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane

Catalysts ◽

10.3390/catal9060495 ◽

2019 ◽

Vol 9 (6) ◽

pp. 495 ◽

Cited By ~ 3

Author(s):

Peng-Cheng Chen ◽

Zhen Ma ◽

Xue-Yan Zhu ◽

Da-Jing Chen ◽

Xiao-Jun Huang

Keyword(s):

Hollow Fiber ◽

High Performance ◽

Hollow Fiber Membrane ◽

Candida Rugosa ◽

Operational Parameters ◽

Membrane Pore ◽

Microfiltration Membranes ◽

Glycerol Triacetate ◽

Operation Pressure ◽

Enzymatic Membrane

Enzymatic membrane bioreactors (EMBRs) possess the characteristic of combining catalysis with separation, and therefore have promising application potentials. In order to achieve a high-performance EMBR, membrane property, as well as operating parameters, should give special cause for concerns. In this work, an EMBR based on hollow fiber polysulfone microfiltration membranes with radial gradient pore structure was fabricated and enzyme immobilization was achieved through pressure-driven filtration. Lipase from Candida rugosa was used for immobilization and EMBR performance was studied with the enzymatic hydrolysis of glycerol triacetate as a model reaction. The influences of membrane pore diameter, substrate feed direction as well as operational parameters of operation pressure, substrate concentration, and temperature on the EMBR activity were investigated with the production of hydrolysates kinetically fitted. The complete EMBR system showed the highest activity of 1.07 × 104 U⋅g−1. The results in this work indicate future efforts for improvement in EMBR.

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