Standardized Seed Extract of Quisqualis indica (HU-033) Attenuates Testosterone Propionate-Induced Benign Prostatic Hyperplasia via α1-Adrenergic Receptors and Androgen/Estrogen Signaling

Abstract Background Benign prostatic hyperplasia (BPH) is a common disease which causes various health problems for elderly men such as urinary retention, recurring urinary tract infection and bladder stones. The aim of this study is to evaluate the therapeutic effects of Juniperus communis L. seed extract (JCS) on BPH in male Wistar rats. Methods To this end, 30 rats were divided into 5 groups (N = 6): group 1 (vehicle), group 2 (disease control), group 3 (standard medicine; 10 mg/kg finasteride), and groups 4 and 5 were treated with 300 mg/kg and 600 mg/kg of the hydroalcoholic JCS seed extract, respectively. Groups 2, 3, 4 and 5 received testosterone enanthate to induce prostatic hyperplasia. At the end of experimental period (28 days), prostate glands were cut off under anesthesia. Histopathological examination was done and biochemical parameters such as Malondialdehyde, Glutathione and protein carbonyl were also measured. Their body weights were also observed during the study. At the end of the experiment, prostate weights and prostate specific antigen (PSA) levels were measured. Prostate index, inhibition prostate weight and inhibition prostate index were also calculated. Results Both histopathological examination and biochemical parameter results showed significant improvements in rats treated with finasteride and 600 mg/kg JCS extract (p < 0.01). In addition, PSA levels showed significant decrease in comparison with the disease group. But acute toxicity test indicated that using JCS extract resulted in an increase in liver enzymes (ALP, LDH, SGOT, SGPT). As a result, the extract should be used with caution. Conclusions Oral administration of JCS extract is effective on preventing testosterone-induced benign prostatic hyperplasia.

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Effects of Pumpkin Seed in Men with Lower Urinary Tract Symptoms due to Benign Prostatic Hyperplasia in the One-Year, Randomized, Placebo-Controlled GRANU Study

Urologia Internationalis ◽

10.1159/000362903 ◽

2014 ◽

Vol 94 (3) ◽

pp. 286-295 ◽

Cited By ~ 21

Author(s):

Winfried Vahlensieck ◽

Christoph Theurer ◽

Edith Pfitzer ◽

Brigitte Patz ◽

Norbert Banik ◽

...

Keyword(s):

Urinary Tract ◽

Benign Prostatic Hyperplasia ◽

Lower Urinary Tract Symptoms ◽

Lower Urinary Tract ◽

Seed Extract ◽

Prostatic Hyperplasia ◽

Secondary Outcome ◽

Pumpkin Seed ◽

Urinary Tract Symptoms ◽

Lower Urinary

Introduction: The German Research Activities on Natural Urologicals (GRANU) study was a randomized, partially blinded, placebo-controlled, parallel-group trial that investigated the efficacy of pumpkin seed in men with lower urinary tract symptoms suggestive of benign prostatic hyperplasia (BPH/LUTS). Subjects and Methods: A total of 1,431 men (50−80 years) with BPH/LUTS were randomly assigned to either pumpkin seed (5 g b.i.d.), capsules with pumpkin seed extract (500 mg b.i.d.) or matching placebo. The primary response criterion was a decrease in International Prostate Symptom Score (IPSS) of ≥5 points from baseline after 12 months. Secondary outcome measures included IPSS-related quality of life, IPSS single items and diary-recorded nocturia. Results: After 12 months, the response rate (intention-to-treat/last-observation-carried-forward approach) did not differ between pumpkin seed extract and placebo. In the case of pumpkin seed (responders: 58.5%), the difference compared with placebo (responders: 47.3%) was descriptively significant. The study products were well tolerated. Overall, in men with BPH, 12 months of treatment with pumpkin seed led to a clinically relevant reduction in IPSS compared with placebo. Conclusion: In order to fully justify a recommendation for the use of pumpkin seed to treat moderate LUTS, these findings need to be substantiated in a confirmatory study or systematic review.

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Effect of an oil-free hydroethanolic pumpkin seed extract on symptoms of benign prostatic hyperplasia

10.1055/s-0039-3399723 ◽

2019 ◽

Author(s):

M Leibbrand ◽

S Siefer ◽

C Schön ◽

T Perrinjaquet-Moccetti ◽

A Kompek ◽

...

Keyword(s):

Benign Prostatic Hyperplasia ◽

Seed Extract ◽

Prostatic Hyperplasia ◽

Pumpkin Seed

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The effects of Sida rhombifoia L. on benign prostatic hyperplasia in testosterone propionate-induced animal model and its active constituents

10.1055/s-0039-3400097 ◽

2019 ◽

Author(s):

EJ Jeong ◽

YN Kim ◽

NH Kim ◽

JD Heo

Keyword(s):

Animal Model ◽

Benign Prostatic Hyperplasia ◽

Testosterone Propionate ◽

Prostatic Hyperplasia ◽

Active Constituents

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Effect of Paecilomyces tenuipes Extract on Testosterone-Induced Benign Prostatic Hyperplasia in Sprague–Dawley Rats

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16193764 ◽

2019 ◽

Vol 16 (19) ◽

pp. 3764 ◽

Cited By ~ 1

Author(s):

Choi ◽

Kim ◽

Fan ◽

Tang ◽

Hwang ◽

...

Keyword(s):

Benign Prostatic Hyperplasia ◽

Rat Model ◽

Testosterone Propionate ◽

Inhibitory Effect ◽

Prostatic Hyperplasia ◽

Control Group ◽

Lncap Cells ◽

Prostate Cell ◽

Expression Levels ◽

Prostate Size

: Benign prostatic hyperplasia (BPH) is one of the major public health concerns, which has a high prevalence rate and causes significant decline in men's quality of life. BPH is highly related to sexual hormone metabolism and aging. In particular, dihydrotestosterone (DHT), to which testosterone is modified by 5α-reductase (5AR), has a significant effect on BPH development. DHT binds to an androgen receptor (AR) and steroid receptor coactivator 1 (SRC-1); then, it induces the proliferation of a prostate cell and expression of prostate specific antigen (PSA). Paecilomyces tenuipes (P. tenuipes) is a mushroom that has been popularized by the artificial cultivation of fruiting bodies based on silkworms by researchers from the Republic of Korea. In a previous study, we identified the effect of PE on PSA mRNA expression in LNCaP cells. This suggests that PE may have an inhibitory effect on androgen signaling. Therefore, we confirmed the expression of androgen signaling-related factors, such as AR, SRC-1, and PSA in LNCaP. Furthermore, we confirmed the androgen signaling inhibitory effect of PE using the testosterone propionate (TP)-induced BPH rat model. A BPH rat model was established with a four-week treatment of daily subcutaneous injections of testosterone propionate (TP, 3 mg/kg) dissolved in corn oil after castration. The rats in the treatment group were orally gavaged P. tenuipes extract (PE), finasteride (Fi), or saw palmetto extract (Saw) with TP injection. DHT induced an increase in the expression levels of AR, SRC-1, and PSA proteins in LNCaP cells. On the contrary, the PE treatment reduced the expression levels. In vivo, the BPH group showed an increase in prostate size compared with the control group. The PE gavaged group showed a decrease in prostate size compared with the BPH group. In addition, the protein expressions of AR, 5AR2, and PSA were significantly lower in the PE gavaged group than BPH group in prostate tissue. These results suggest the beneficial effects of PE on BPH via the modulation of AR signaling pathway.

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1576 A PROSPECTIVE STUDY OF THE EFFECTS OF DOXAZOSIN ON ALPHA 1-ADRENERGIC RECEPTORS IN PROSTATES WITH BENIGN PROSTATIC HYPERPLASIA

The Journal of Urology ◽

10.1016/j.juro.2011.02.1612 ◽

2011 ◽

Vol 185 (4S) ◽

Author(s):

Ha Na Lee ◽

Bong Suk Shim ◽

Jeong Hee Hong ◽

Won Jin Cho ◽

Deok Hyun Han ◽

...

Keyword(s):

Benign Prostatic Hyperplasia ◽

Prospective Study ◽

Adrenergic Receptors ◽

Prostatic Hyperplasia ◽

A Prospective Study

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Aconiti Lateralis Radix Preparata, the Dried Root of Aconitum carmichaelii Debx., Improves Benign Prostatic Hyperplasia via Suppressing 5-Alpha Reductase and Inducing Prostate Cell Apoptosis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/6369132 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Jinbong Park ◽

Dong-Hyun Youn ◽

Jae-Young Um

Keyword(s):

Benign Prostatic Hyperplasia ◽

Therapeutic Agent ◽

Testosterone Propionate ◽

Prostatic Hyperplasia ◽

Common Disease ◽

Elderly Men ◽

Prostate Cell ◽

Al Treatment ◽

Aconitum Carmichaelii ◽

Lower Urinary

Benign prostatic hyperplasia (BPH) is a common disease in elderly men which can be characterized by an abnormal enlargement of the prostate associated with lower urinary symptoms. Current medications available for BPH treatment display several adverse effects; thus, the search for effective treatments with less side effects is still ongoing. In this study, we investigated the effect of Aconiti Lateralis Radix Preparata (dried root of Aconitum carmichaelii Debx.; AL), which is an herb used to treat extremely cold symptoms in traditional Korean medicine, on BPH using a testosterone propionate- (TP-) induced BPH rat model. Eight-week inguinal injection of TP induced BPH in rats, the prostate of which was displaying an abnormal proliferation. The pathological proliferation of the prostate was ameliorated by AL treatment of 4 weeks. Pathohistological changes in the prostate including epithelial thickness and lumen area were restored in AL-treated rats. Furthermore, 5α-reductase (5AR) and androgen receptor (AR), the two main factors in the pathogenesis of BPH, were decreased. In addition, the ratio of BAX and Bcl-2, an indicator of apoptosis, was increased by AL as well. Similar results were observed in AL-treated LNCaP prostate cancer cells. AL treatment suppressed the expression of the 5AR-AR axis and increased the ratio of BAX and Bcl-2. Apoptosis in the testis is considered a crucial side effect of finasteride, a 5AR inhibitor used to treat BPH. Our results showed that AL treatment did not display such effects, while finasteride treatment resulted in loss of spermatogenic cells within the prostate. Overall, these results suggest AL as a potentially safe nature-derived therapeutic agent for BPH treatment.

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