scholarly journals Antracología de una unidad residencial de el Pichao (Tucumán-Argentina)

2020 ◽  
Vol 24 (2) ◽  
pp. 5-28
Author(s):  
María Gabriela Aguirre ◽  
María Marta Sampietro Vattuone ◽  
Maia del Rosario Rodríguez ◽  
Sergio Fabián Cano
Keyword(s):  
Nicotiana Glauca ◽  
Larrea Divaricata

El sitio arqueológico El Pichao ha sido estudiado desde diversos aspectos, sin embargo, hasta la actualidad no se había llevado a cabo el análisis antracológico de las muestras recuperadas en unidades residenciales del período de Desarrollos Regionales (1000-535 AP). Este tema se aborda mediante el estudio de carbones obtenidos en la unidad STucTav5 - 100. La identificación taxonómica de los macro restos se realizó mediante la comparación de los carbones arqueológicos con una colección de referencia de cortes histológicos que incluye ejemplares de la flora del área de estudio; los carbones se examinaron observando las secciones transversal, longitudinal tangencial y longitudinal radial del leño bajo lupa binocular, microscopio óptico con luz incidente y se tomaron fotografías con microscopio electrónico de barrido. En el conjunto de carbones se identificaron a los siguientes taxa: Prosopis sp., P. torquata, Geoffroea decorticans, Acacia sp. Nicotiana glauca, Bulnesia schickendantzii, Larrea divaricata y un taxón quedó en la categoría de No Identificado. Los mismos forman parte de la flora local del área y habrían sido usados durante ocupaciones correspondientes al período de Desarrollos Regionales y posteriores. Los datos generados alientan al desarrollo de esta línea de trabajo en el área.

scholarly journals Tobacco mild green mosaic virus found naturally infecting Nicotiana glauca in Brazil

2019 ◽  
Vol 14 (1) ◽  
Author(s):  
Gabriel Madoglio Favara ◽  
Viviana Marcela Camelo-García ◽  
Tatsuya Nagata ◽  
João Marcos Fagundes Silva ◽  
Mariana Saito ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Nicotiana Glauca

scholarly journals The Presence and Characterization of a virF Gene on Agrobacterium vitis Ti Plasmids

1998 ◽  
Vol 11 (5) ◽  
pp. 429-433 ◽  
Author(s):  
B. Schrammeijer ◽  
J. Hemelaar ◽  
P. J. J. Hooykaas
Keyword(s):  
Amino Acids ◽  
Agrobacterium Tumefaciens ◽  
Molecular Mass ◽  
Promoter Region ◽  
Consensus Sequence ◽  
Tumor Formation ◽  
Nicotiana Glauca ◽  
Agrobacterium Vitis ◽  
Ti Plasmids

Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N. glauca a host plant for these strains, indicating that the VirF protein is a host-range determinant. Here we show the presence of a virF locus not only on the Agrobacterium vitis octopine/cucumopine plasmids pTiAg57 and pTiTm4, but also on the nopaline Ti plas-mids pTiAT1, pTiAT66a, and pTiAT66b. On the octopine Ti plasmids from A. tumefaciens the virF gene is located between the virE locus and the left border of the T-region. In contrast, the virF gene on Ti plasmids of A. vitis is located at the very left end of the vir-region near the virA locus. The virF gene of pTiAg57 has been sequenced and codes for a protein of 202 amino acids with a molecular mass of 22,280 Da. Comparison showed that the virF gene from A. vitis strain Ag57 is almost identical to that from A. tumefaciens octopine strains. The transcription of the pTiAg57 virF is inducible by the plant phenolic compound acetosyringone through the presence of a vir-box consensus sequence in its promoter region. The VirF protein from pTiAg57 can complement octopine A. tumefaciens strains deleted for virF as shown by tumor formation on N. glauca.

Response to cutting of Larrea divaricata and L. cuneifolia in Argentina

2002 ◽  
Vol 5 (1) ◽  
pp. 127-133 ◽  
Author(s):  
E. Martinez Carretero ◽  
A. Dalmasso
Keyword(s):  
Larrea Divaricata

Analysis of wound-induced gene expression in Nicotiana species with contrasting alkaloid profiles

2004 ◽  
Vol 31 (7) ◽  
pp. 721 ◽  
Author(s):  
Steven J. Sinclair ◽  
Richard Johnson ◽  
John D. Hamill
Keyword(s):  
Fold Increase ◽  
Transcript Abundance ◽  
Nicotiana Sylvestris ◽  
Nicotiana Glauca ◽  
Transcriptional Responses ◽  
Transcript Levels ◽  
Solanaceae Species ◽  
Genes Encoding ◽  
Root Tissues ◽  
Alkaloid Profiles

We determined the capacity of three Nicotiana (Solanaceae) species with very different alkaloid profiles (Nicotiana sylvestris Speg & Comes, Nicotiana alata Link & Otto and Nicotiana glauca Grah.) to increase their alkaloid contents in both leaf and root tissues following foliage damage. We also investigated the transcriptional responses of genes encoding enzymes important for alkaloid biosynthesis, namely quinolinate phosphoribosyltransferase (QPT), putrescine N-methyltransferase (PMT), ornithine decarboxylase (ODC) and the putative alkaloid biosynthetic gene A622. In response to wounding of foliage in the well studied ‘model’ species N. sylvestris, a rise, approximately 2-fold, in leaf nicotine levels was observed several days after a 4–5-fold increase in the transcript levels of all genes in the roots. In contrast, leaf tissues of the ornamental tobacco N. alata showed very low levels of any pyridine alkaloid, even when analysed 1 week after wounding, correlating with a general lack of transcript abundance representing any of these genes in leaves or roots following foliage damage. However, addition of methyl jasmonate to cultured roots of N. alata did produce elevated levels of nicotine and anatabine raising the possibility that components of the leaf–root wound signalling system in N. alata are different from those in N. sylvestris. Wounding of the tree tobacco N. glauca, was followed by a 2-fold increase in anabasine levels several days later. This increase followed a large rise in transcript levels of ODC, QPT and A622, though not PMT, in wounded leaves, but not in non-wounded leaves or roots. These data support the hypothesis that N. glauca is able to produce increased anabasine levels following wounding in its foliage, setting it apart from N. sylvestris where induced alkaloid production takes place in roots. We discuss the possibility that increased transcript levels detected by ODC and A622 probes play important roles in anabasine synthesis in N. glauca.

Reproductive ecology of the bird-pollinated Nicotiana glauca across native and introduced ranges with contrasting pollination environments

2019 ◽  
Vol 22 (2) ◽  
pp. 485-498 ◽  
Author(s):  
E. A. Issaly ◽  
A. N. Sérsic ◽  
A. Pauw ◽  
A. A. Cocucci ◽  
A. Traveset ◽  
...  
Keyword(s):  
Reproductive Ecology ◽  
Nicotiana Glauca ◽  
Native And Introduced Ranges

scholarly journals UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca

2021 ◽  
Vol 72 (1) ◽  
pp. 97-108
Author(s):  
Rafeef K. Massadeh ◽  
Tamam El-Elimat ◽  
Mohammad Al-Gharaibeh ◽  
Khaled Tawaha ◽  
Feras Q. Alali
Keyword(s):  
High Resolution ◽  
Plant Material ◽  
Methanolic Extract ◽  
Volatile Oil ◽  
Ethyl Linoleate ◽  
Major Constituent ◽  
Hexadecanoic Acid ◽  
Nicotiana Glauca ◽  
Plant Oil ◽  
Ms Analysis

Abstract The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g−1 dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of N. glauca. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (e.g., β-bisabolol) and carboxylic acids and esters (e.g., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.

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