EPIGENOMIC FACTORS FOR THE DEVELOPMENT OF PERITONEAL ADHESIONS

Summary. The etiology of the development of adhesive disease — the most common postoperative complication — can be determined by epigenomic disorders related to various links of resistance and immunogenetic control. Purpose of the study. To study the epigenomic factors in the development of adhesive disease, the immune status of patients operated on in the abdominal cavity was studied. The comparison group included 55 patients with a complicated course of peritoneal commissural disease, the main group consisted of 59 patients operated on on the abdominal organs on the background of peritoneal commissural disease, the course of which was asymptomatic. Results and its discussion. The revealed epigenomic factors associated with the risk of developing adhesive disease: belongs to an increase in the concentration of acute phase proteins - ceruloplasmin, haptoglobin, C-reactive protein, C3 fragment of the complement system proteins, changes in the expression of adhesion molecule genes (CD31 increased by 10 %, CD54 increased to 24.1 % in comparison with the comparison group — 13.25 %). Conclusions. The results of our studies showed that patients with adhesive disease have a wide range of epigenome trigger factors. Epigenomic factors associated with the risk of developing adhesive disease include an increase in the concentration of acute phase proteins - ceruloplasmin, haptoglobin, C-reactive protein, C3 fragment of the complement system proteins, changes in the expression of adhesion molecule genes (CD31 increased by 10 %, CD54 increased to 24, 1 % compared with the comparison group - 13.25 %)

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The acute phase response and C-reactive protein

Oxford Textbook of Medicine ◽

10.1093/med/9780198746690.003.0239 ◽

2020 ◽

pp. 2199-2207

Author(s):

Mark B. Pepys

Keyword(s):

Acute Phase ◽

Acute Phase Response ◽

Serum Amyloid A ◽

Acute Phase Proteins ◽

Phase Response ◽

Serum Amyloid ◽

C Reactive Protein ◽

Reactive Protein ◽

Amyloid A ◽

Serum Amyloid A Protein

The acute phase response—trauma, tissue necrosis, infection, inflammation, and malignant neoplasia induce a complex series of nonspecific systemic, physiological, and metabolic responses including fever, leucocytosis, catabolism of muscle proteins, greatly increased de novo synthesis and secretion of a number of ‘acute phase’ plasma proteins, and decreased synthesis of albumin, transthyretin, and high- and low-density lipoproteins. The altered plasma protein concentration profile is called the acute phase response. Acute phase proteins—these are mostly synthesized by hepatocytes, in which transcription is controlled by cytokines including interleukin 1, interleukin 6, and tumour necrosis factor. The circulating concentrations of complement proteins and clotting factors increase by up to 50 to 100%; some of the proteinase inhibitors and α‎1-acid glycoprotein can increase three- to fivefold; but C-reactive protein (CRP) and serum amyloid A protein (an apolipoprotein of high-density lipoprotein particles) are unique in that their concentrations can change by more than 1000-fold. C-reactive protein—this consists of five identical, nonglycosylated, noncovalently associated polypeptide subunits. It binds to autologous and extrinsic materials which contain phosphocholine, including bacteria and their products. Ligand-bound CRP activates the classical complement pathway and triggers the inflammatory and opsonizing activities of the complement system, thereby contributing to innate host resistance to pneumococci and probably to recognition and safe ‘scavenging’ of cellular debris. Clinical features—(1) determination of CRP in serum or plasma is the most useful marker of the acute phase response in most inflammatory and tissue damaging conditions. (2) Acute phase proteins may be harmful in some circumstances. Sustained increased production of serum amyloid A protein can lead to the deposition of AA-type, reactive systemic amyloid.

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Acute phase proteins in bitches subjected to conventional and minimally invasive ovariohysterectomy

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5132 ◽

2018 ◽

Vol 38 (11) ◽

pp. 2124-2128 ◽

Cited By ~ 1

Author(s):

Elizabeth M.S. Schmidt ◽

Camila P. Rubio ◽

Funmilola Thomas ◽

João C.P. Ferreira ◽

David P. Eckersall

Keyword(s):

Minimally Invasive ◽

Acute Phase ◽

Acute Phase Proteins ◽

C Reactive Protein ◽

Reactive Protein ◽

Serum Haptoglobin ◽

Hemoglobin Binding ◽

Mixed Breed ◽

Significant Difference ◽

Haptoglobin Concentration

ABSTRACT: The aim of this study was to evaluate and to compare the possible inflammatory changes by screening acute phase proteins concentrations in healthy bitches subjected to ovariohysterectomy. Minimally invasive and conventional (laparotomy) ovariohysterectomies were performed in 17 client-owned adult female mixed breed dogs. Nine animals were subjected to minimally invasive and eight animals to conventional ovariohysterectomy. Blood samples were taken before surgery, 24, 48 hours, and seven days postoperatively. Serum C-reactive concentration was determined by a commercial ELISA kit and serum haptoglobin concentration was measured via hemoglobin binding assay, both previously validated for use in dogs. As the data did not meet the normal distribution criteria, the nonparametric Kruskall-Wallis was performed to compare quantitative variables between groups. One-way ANOVA and the Friedman test were used for multiple comparisons between time points, with a P<0.05 considered significant. C-reactive protein concentration was significantly different (P<0.0001) at 24 hours postoperatively between groups. There was no significant difference in haptoglobin concentration between groups. C-reactive protein and haptoglobin concentrations were significantly different at 24 and 48 hours postoperatively for minimally invasive and conventional ovariohisterectomies. These findings provided an overview of the short-term inflammatory effects produced by minimally invasive and conventional ovariohysterectomies.

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Luminometric assays of seven acute-phase proteins in minimal volumes of serum, plasma, sputum, and bronchioalveolar lavage.

Clinical Chemistry ◽

10.1093/clinchem/32.5.743 ◽

1986 ◽

Vol 32 (5) ◽

pp. 743-747 ◽

Cited By ~ 13

Author(s):

J Braun ◽

T Schultek ◽

K F Tegtmeier ◽

A Florenz ◽

C Rohde ◽

...

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Immunoglobulin A ◽

C Reactive Protein ◽

Acid Glycoprotein ◽

Reactive Protein ◽

Coefficients Of Variation ◽

Alpha 1 Antitrypsin ◽

Heel Prick ◽

Secretory Immunoglobulin

Abstract We describe immunoluminometric assays for seven acute-phase proteins, which can be determined in minimal volumes of plasma, serum, sputum, and bronchioalveolar lavage. The theoretical volume of serum or plasma required to measure all seven analytes in duplicate is 130 nL, although in practice the smallest volume of sample was enough to fill a hematocrit tube (about 25 microL of blood), collected from neonates by the heel-prick method. The assays could be performed with 10 microL of sputum or with 100 microL of bronchioalveolar lavage. We measured alpha 1-antitrypsin, alpha 2-macroglobulin, alpha 1-acid glycoprotein, thyroxin-binding prealbumin, C-reactive protein, and total and secretory immunoglobulin A. The assays are rapid enough for all results to be returned to the ward on the same day and are suitable for monitoring neonatal sepsis. All coefficients of variation, derived from compound precision profiles, were less than 7% for clinically relevant analyte concentrations. Correlation with commercially available nephelometric assays was good.

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Autoantibodies to C-reactive protein (CRP) and other acute-phase proteins in systemic autoimmune diseases

Clinical & Experimental Immunology ◽

10.1046/j.1365-2249.1998.00655.x ◽

1998 ◽

Vol 113 (3) ◽

pp. 327-332 ◽

Cited By ~ 52

Author(s):

Bell ◽

Faust ◽

Schmid ◽

Meurer

Keyword(s):

Autoimmune Diseases ◽

Acute Phase ◽

Acute Phase Proteins ◽

C Reactive Protein ◽

Systemic Autoimmune Diseases ◽

Reactive Protein

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Autoantibodies to C-reactive protein and other acute phase proteins in systemic autoimmune diseases

Journal of Dermatological Science ◽

10.1016/s0923-1811(98)84140-6 ◽

1998 ◽

Vol 16 ◽

pp. S191

Author(s):

Susanne A. Bell ◽

Anja Schmid ◽

Michael Meurer

Keyword(s):

Autoimmune Diseases ◽

Acute Phase ◽

Acute Phase Proteins ◽

C Reactive Protein ◽

Systemic Autoimmune Diseases ◽

Reactive Protein

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Abstract B87: Acute-phase proteins (C-reactive protein and Serum Amyloid A) and post-diagnosis mammographic density in breast cancer survivors

Cancer Prevention Research ◽

10.1158/1940-6207.prev-12-b87 ◽

2012 ◽

Vol 5 (11 Supplement) ◽

pp. B87-B87

Author(s):

Anne Dee ◽

Roberta McKean-Cowdin ◽

Anne McTiernan ◽

Richard N. Baumgartner ◽

Kathy B. Baumgartner ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Survivors ◽

Mammographic Density ◽

Acute Phase ◽

Breast Cancer Survivors ◽

Serum Amyloid A ◽

Acute Phase Proteins ◽

C Reactive Protein ◽

Reactive Protein ◽

Amyloid A

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Evolutionary changes in acute-phase proteins in alcoholic hepatocellular diseases.

Clinical Chemistry ◽

10.1093/clinchem/29.1.45 ◽

1983 ◽

Vol 29 (1) ◽

pp. 45-47 ◽

Cited By ~ 4

Author(s):

C Perier ◽

A Chamson ◽

R Engler ◽

J Frey

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Hepatic Disease ◽

Serum Transferrin ◽

Chronic Alcohol ◽

C Reactive Protein ◽

Chronic Alcohol Consumption ◽

Reactive Protein ◽

Evolutionary Changes ◽

Two Stages

Abstract We studied the pattern of acute-phase proteins (orosomucoid, C-reactive protein, and haptoglobin) in hepatocellular deficiency due to chronic alcohol consumption, characterized by a decrease in serum transferrin concentration. We found that their patterns could vary independently of hepatocellular deficiency, but depend on the progression of hepatic disease. The most useful protein for discriminating the stage of inflammatory reaction is orosomucoid. In moderate hepatocellular deficiency, acute-phase proteins are increased independently of the decrease in transferrin, whereas in severe hepatocellular deficiency the acute-phase proteins are also decreased. Thus, it is possible to distinguish the two stages of hepatocellular deficiency by following changes in the concentration of orosomucoid.

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Concentrations of tumor-associated trypsin inhibitor and C-reactive protein in serum in acute pelvic inflammatory disease.

Clinical Chemistry ◽

10.1093/clinchem/35.5.869 ◽

1989 ◽

Vol 35 (5) ◽

pp. 869-871 ◽

Cited By ~ 12

Author(s):

J Paavonen ◽

M Lehtinen ◽

M Lehto ◽

S Laine ◽

R Aine ◽

...

Keyword(s):

Pelvic Inflammatory Disease ◽

Acute Phase ◽

Trypsin Inhibitor ◽

Inflammatory Disease ◽

Acute Phase Proteins ◽

Endometrial Biopsy ◽

C Reactive Protein ◽

Reactive Protein ◽

Acute Pelvic Inflammatory Disease ◽

Severe Infections

Abstract We measured tumor-associated trypsin inhibitor (TATI) and C-reactive protein (CRP) in serum of 29 patients with proven pelvic inflammatory disease (PID). TATI values were increased in seven (24%), paralleling increases in CRP. TATI was increased by about 3.5-fold in seven of eight patients with CRP concentrations greater than 90 mg/L, but in none of 21 patients with CRP concentrations less than 90 mg/L. TATI concentration and severity of PID as determined by laparoscopy or endometrial biopsy were not correlated. These results suggest that, in severe infections, regulation of TATI synthesis resembles that of acute-phase proteins.

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