scholarly journals Polymerase chain reaction detection methods of Survival Motor Neuron genes: a review

2020 ◽  
Vol 1 (1) ◽  
pp. 37-43
Author(s):  
Azra Alimanović ◽  
Jasmin Šutković
Keyword(s):  
Polymerase Chain Reaction ◽  
Spinal Muscular Atrophy ◽  
Motor Neuron ◽  
Muscular Atrophy ◽  
Quantitative Polymerase Chain Reaction ◽  
Survival Motor Neuron ◽  
Detection Methods ◽  
Chain Reaction ◽  
Pcr Rflp ◽  
Polymerase Chain

Two SMN (survival motor neuron) genes are presented in the human genome: SMN1,  which present the telomeric gene whose homozygous deletion or mutation like gene conversion, causes spinal muscular atrophy (SMA), and SMN2, the centromeric version whose copy number modulates the phenotype of SMA These genes are commonly detected by Polymerase Chain reaction-based methods, and these are MLPA (Multiplex ligation-dependent probe amplification), qPCR (quantitative Polymerase chain reaction) and PCR-RFLP (Polymerase chain reaction-Restriction fragment length polymorphism). This paper reviews the current standing of the most common PCR methods used in the detection of spinal muscular atrophy genes. MLPA, qPCR, and PCR-RFLP currently represent the most common methods of choice for the detection of mutations, especially for deletion and duplication mutations.

Detection of Spinal Muscular Atrophy Carriers by Nested Polymerase Chain Reaction of Single Sperm Cells

2006 ◽  
Vol 10 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Yuval Yaron ◽  
Tania Cohen ◽  
Nava Mey-Raz ◽  
Tamar Schwartz ◽  
Ami Amit ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Spinal Muscular Atrophy ◽  
Muscular Atrophy ◽  
Sperm Cells ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Event-Specific Qualitative and Quantitative Polymerase Chain Reaction Methods for Detection of Genetically Modified Rapeseed Ms8Rf3 Based on the Right Border Junctions

2008 ◽  
Vol 91 (1) ◽  
pp. 143-151 ◽  
Author(s):  
Gang Wu ◽  
Yuhua Wu ◽  
Ling Xiao ◽  
Changming Lu
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetically Modified ◽  
Quantitative Polymerase Chain Reaction ◽  
Artificial Chromosome ◽  
Absolute Quantification ◽  
Detection Methods ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
The Absolute ◽  
The Right

Abstract Ms8Rf3 is a genetically modified rapeseed hybrid which is widely cultivated in Canada and exported to some other countries for production of foodstuffs or fodder. In this study, the genomic sequences flanking the right borders of the integrated transgenic sequences in the Ms8Rf3 genome were characterized and showed high similarities with the bacterial artificial chromosome clone of Chinese cabbage. Event-specific qualitative polymerase chain reaction (PCR) methods were established with the primers and probes targeting the junction regions to produce a 123 base pair (bp) product for the Ms8 event and 92 bp for the Rf3 event. The absolute detection limit of qualitative PCR was 2.5 initial template copies for the Ms8 event and 50 copies for the Rf3 event. Quantitiative detection methods were established, with the absolute quantification limit being approximately 25 initial template copies.

Effect of zinc pyrithione shampoo treatment on skin commensal Malassezia

2020 ◽  
Author(s):  
Cheryl Leong ◽  
Joyce Wang ◽  
Min Jet Toi ◽  
Yuen In Lam ◽  
Joleen P Z Goh ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Polymerase Chain Reaction ◽  
Detection Methods ◽  
Chain Reaction ◽  
Zinc Pyrithione ◽  
Qpcr Analysis ◽  
Malassezia Globosa ◽  
Polymerase Chain ◽  
Antifungal Efficacy ◽  
Malassezia Restricta

Abstract Malassezia restricta and Malassezia globosa are lipid dependent commensal yeasts associated with dandruff. Antifungal actives such as zinc pyrithione are commonly used in antidandruff shampoos, although their efficacy is not clearly demonstrated. In this study, we assessed the efficacy of antifungal treatments on scalp Malassezia via a combination of culturomic and genomic detection methods. Zinc pyrithione inhibited Malassezia growth at low minimum inhibitory concentrations (MICs). In a longitudinal pilot study, quantitative polymerase chain reaction (qPCR) analysis showed a decrease in M. restricta on the scalp after zinc pyrithione treatment. These findings validate the antifungal efficacy of zinc pyrithione as a dandruff treatment.

AN IMPROVED METHOD FOR INHIBITOR FREE NUCLEIC ACIDS FROM POLYPHENOL RICH LEAVES OF MUSA SPP

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
N.NANDHA KUMAR ◽  
K. SOURIANATHA SUNDARAM ◽  
D. SUDHAKAR ◽  
K.K. KUMAR
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Polymerase Chain Reaction ◽  
Proteinase K ◽  
Chain Reaction ◽  
Banana Plant ◽  
High Molecular Weight Dna ◽  
Musa Spp ◽  
Leaf Tissues ◽  
Polymerase Chain

Excessive presence of polysaccharides, polyphenol and secondary metabolites in banana plant affects the quality of DNA and it leads to difficult in isolating good quality of DNA. An optimized modified CTAB protocol for the isolation of high quality and quantity of DNA obtained from banana leaf tissues has been developed. In this protocol a slight increased salt (NaCl) concentration (2.0M) was used in the extraction buffer. Polyvinylpyrrolidone (PVP) and Octanol were used for the removal of polyphenols and polymerase chain reaction (PCR) inhibitors. Proteins like various enzymes were degraded by Proteinase K and removed by centrifugation from plant extract during the isolation process resulting in pure genomic DNA, ready to use in downstream applications including PCR, quantitative polymerase chain reaction (qPCR), ligation, restriction and sequencing. This protocol yielded a high molecular weight DNA isolated from polyphenols rich leaves of Musa spp which was free from contamination and colour. The average yields of total DNA from leaf ranged from 917.4 to 1860.9 ng/ìL. This modified CTAB protocol reported here is less time consuming 4-5h, reproducible and can be used for a broad spectrum of plant species which have polyphenol and polysaccharide compounds.

Sign in / Sign up

Export Citation Format

Share Document