scholarly journals Event-Specific Qualitative and Quantitative Polymerase Chain Reaction Methods for Detection of Genetically Modified Rapeseed Ms8Rf3 Based on the Right Border Junctions

2008 ◽  
Vol 91 (1) ◽  
pp. 143-151 ◽  
Author(s):  
Gang Wu ◽  
Yuhua Wu ◽  
Ling Xiao ◽  
Changming Lu
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetically Modified ◽  
Quantitative Polymerase Chain Reaction ◽  
Artificial Chromosome ◽  
Absolute Quantification ◽  
Detection Methods ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
The Absolute ◽  
The Right

Abstract Ms8Rf3 is a genetically modified rapeseed hybrid which is widely cultivated in Canada and exported to some other countries for production of foodstuffs or fodder. In this study, the genomic sequences flanking the right borders of the integrated transgenic sequences in the Ms8Rf3 genome were characterized and showed high similarities with the bacterial artificial chromosome clone of Chinese cabbage. Event-specific qualitative polymerase chain reaction (PCR) methods were established with the primers and probes targeting the junction regions to produce a 123 base pair (bp) product for the Ms8 event and 92 bp for the Rf3 event. The absolute detection limit of qualitative PCR was 2.5 initial template copies for the Ms8 event and 50 copies for the Rf3 event. Quantitiative detection methods were established, with the absolute quantification limit being approximately 25 initial template copies.

Event-specific quantitative polymerase chain reaction methods for detection of double-herbicide-resistant genetically modified corn MON 87419 based on the 3′-junction of the insertion site

2021 ◽  
Author(s):  
Likun Long ◽  
Wei Yan ◽  
Congcong Li ◽  
Liming Dong ◽  
Na Liu ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetically Modified ◽  
Quantitative Polymerase Chain Reaction ◽  
Insertion Site ◽  
Chain Reaction ◽  
Herbicide Resistant ◽  
Qualitative Polymerase Chain Reaction ◽  
Relative Standard ◽  
Junction Site ◽  
Polymerase Chain

ABSTRACT MON 87419 was one of the new transgenic corn events developed in US with the trait of herbicide resistance to both dicamba and glyphosate. To monitor unintended release of genetically modified organism in the future, as well as to meet GM-labeling requirements, it is requisite to develop a reliable method for the detection and quantification of MON 87419, an event-specific primer pair was designed to amplify the 3′-junction site between the endogenous genome sequence and the transferred DNA of GM event MON 87419, amplicons of desired size were produced by qualitative polymerase chain reaction (PCR) assay. For the validation of this quantitative method, the mixed samples containing 10%, 1%, and 0.1% MON 87419 ingredient were quantified. The precisions were expressed as relative standard deviations, deviated by 7.87%, 12.94%, and 19.98%, respectively. These results clearly demonstrate that the PCR methods we developed herein can be used for event-specific quantitative testing of the double-herbicide-resistant corn MON 87419.

Detection of nonauthorized genetically modified organisms using differential quantitative polymerase chain reaction: application to 35S in maize

2008 ◽  
Vol 376 (2) ◽  
pp. 189-199 ◽  
Author(s):  
Katarina Cankar ◽  
Valérie Chauvensy-Ancel ◽  
Marie-Noelle Fortabat ◽  
Kristina Gruden ◽  
André Kobilinsky ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Real-Time Quantitative Polymerase Chain Reaction Methods for Four Genetically Modified Maize Varieties and Maize DNA Content in Food

2002 ◽  
Vol 85 (3) ◽  
pp. 646-653 ◽  
Author(s):  
Peter D Brodmann ◽  
Evelyn C Ilg ◽  
Hélène Berthoud ◽  
André Herrmann
Keyword(s):  
European Union ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Genetically Modified ◽  
Detection Methods ◽  
Genetically Modified Maize ◽  
The European Union ◽  
Chain Reaction ◽  
Maize Varieties ◽  
Polymerase Chain

Abstract Quantitative detection methods are needed for enforcement of the recently introduced labeling threshold for genetically modified organisms (GMOs) in food ingredients. This labeling threshold, which is set to 1% in the European Union and Switzerland, must be applied to all approved GMOs. Four different varieties of maize are approved in the European Union: the insect-resistant Bt176 maize (Maximizer), Bt11 maize, Mon810 (YieldGard) maize, and the herbicide-tolerant T25 (Liberty Link™) maize. Because the labeling must be considered individually for each ingredient, a quantitation system for the endogenous maize content is needed in addition to the GMO-specific detection systems. Quantitative real-time polymerase chain reaction detection methods were developed for the 4 approved genetically modified maize varieties and for an endogenous maize (invertase) gene system.

scholarly journals Polymerase chain reaction detection methods of Survival Motor Neuron genes: a review

2020 ◽  
Vol 1 (1) ◽  
pp. 37-43
Author(s):  
Azra Alimanović ◽  
Jasmin Šutković
Keyword(s):  
Polymerase Chain Reaction ◽  
Spinal Muscular Atrophy ◽  
Motor Neuron ◽  
Muscular Atrophy ◽  
Quantitative Polymerase Chain Reaction ◽  
Survival Motor Neuron ◽  
Detection Methods ◽  
Chain Reaction ◽  
Pcr Rflp ◽  
Polymerase Chain

Two SMN (survival motor neuron) genes are presented in the human genome: SMN1,  which present the telomeric gene whose homozygous deletion or mutation like gene conversion, causes spinal muscular atrophy (SMA), and SMN2, the centromeric version whose copy number modulates the phenotype of SMA These genes are commonly detected by Polymerase Chain reaction-based methods, and these are MLPA (Multiplex ligation-dependent probe amplification), qPCR (quantitative Polymerase chain reaction) and PCR-RFLP (Polymerase chain reaction-Restriction fragment length polymorphism). This paper reviews the current standing of the most common PCR methods used in the detection of spinal muscular atrophy genes. MLPA, qPCR, and PCR-RFLP currently represent the most common methods of choice for the detection of mutations, especially for deletion and duplication mutations.

Sign in / Sign up

Export Citation Format

Share Document