scholarly journals Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells

Author(s):  
Kristian Pajtler ◽  
Anna Bohrer ◽  
Jochen Maurer ◽  
Hubert Schorle ◽  
Alexander Schramm ◽  
...  



2021 ◽  
Author(s):  
Hyun‐Mun Kim ◽  
Hye Bin Noh ◽  
Sang‐Hyuk Lee ◽  
Kun‐Gu Lee ◽  
Bomi Chang ◽  
...  




Neuron ◽  
2002 ◽  
Vol 35 (4) ◽  
pp. 657-669 ◽  
Author(s):  
Genevieve M. Kruger ◽  
Jack T. Mosher ◽  
Suzanne Bixby ◽  
Nancy Joseph ◽  
Toshihide Iwashita ◽  
...  


2021 ◽  
pp. 102476
Author(s):  
Valerio Brizi ◽  
Sara Buttò ◽  
Domenico Cerullo ◽  
Angelo Michele Lavecchia ◽  
Raquel Rodrigues-Diez ◽  
...  


2015 ◽  
Vol 2 (4) ◽  
pp. 275-281 ◽  
Author(s):  
Atsushi Tomokiyo ◽  
Kim Hynes ◽  
Stan Gronthos ◽  
Naohisa Wada ◽  
P. Mark Bartold


1974 ◽  
Vol 61 (2) ◽  
pp. 369-382 ◽  
Author(s):  
D. A. Newsome ◽  
R. T. Fletcher ◽  
W. G. Robison ◽  
K. R. Kenyon ◽  
G. J. Chader

The effects of dibutyryl cyclic 3',5'-adenosine monophosphate (BcAMP) and Sephadex G-25 fractions of chick embryo extract on the growth rate, morphology, and pigmentation of normal chick retinal pigmented epithelium (PE) were investigated. Seven cloned PE cell lines were each grown in modified Ham's F-12 medium alone (F-12), or in F-12 supplemented with either high molecular weight (H) or low molecular weight (L) fractions of chick embryo extract. Cells grown in F-12 alone or in L medium formed compact epithelial sheets, whereas cells grown in H had a fibrocytic appearance and formed poorly organized monolayers. In H plus BcAMP, cell morphology was more epithelioid than in H alone, and generally the monolayers appeared more differentiated. Under each of these three culture conditions, 2 x 10-4 M BCAMP retarded the increase in cell number and decreased the final number of cells per culture dish, but had little effect on plating efficiency. BcAMP also increased the rate of cell adhesion to a plastic substratum. Pigmentation was marked in cultures grown in F-12 or in L alone, but the addition of BcAMP dramatically reduced visible pigmentation. This effect was reversed when BcAMP was removed from the culture medium. Thus BcAMP modifies cell and colonial morphology, rate of cell accumulation, adhesive properties, and pigmentation of normal PE cells.



Stem Cells ◽  
2014 ◽  
Vol 32 (4) ◽  
pp. 829-843 ◽  
Author(s):  
Virginie Neirinckx ◽  
Dorothée Cantinieaux ◽  
Cécile Coste ◽  
Bernard Rogister ◽  
Rachelle Franzen ◽  
...  


Cytotherapy ◽  
2015 ◽  
Vol 17 (6) ◽  
pp. S37
Author(s):  
Cécile Coste ◽  
Virginie Neirinckx ◽  
Anil Sharma ◽  
Bernard Rogister ◽  
François Lallemend ◽  
...  


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