scholarly journals Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice

10.3791/2618 ◽  
2011 ◽  
Author(s):  
Lauren H. Sansing ◽  
Scott E. Kasner ◽  
Louise McCullough ◽  
Puneet Agarwal ◽  
Frank A. Welsh ◽  
...  
Keyword(s):  
Intracerebral Hemorrhage ◽  
Autologous Blood ◽  
Spontaneous Intracerebral Hemorrhage ◽  
Autologous Blood Injection ◽  
Blood Injection

scholarly journals Serial quantitative neuroimaging of iron in the intracerebral hemorrhage pig model

2018 ◽  
Vol 38 (3) ◽  
pp. 375-381 ◽  
Author(s):  
Muhammad E Haque ◽  
Refaat E Gabr ◽  
Xiurong Zhao ◽  
Khader M Hasan ◽  
Andrew Valenzuela ◽  
...  
Keyword(s):  
Intracerebral Hemorrhage ◽  
Brain Damage ◽  
Autologous Blood ◽  
Susceptibility Mapping ◽  
Pig Model ◽  
Quantitative Susceptibility Mapping ◽  
Autologous Blood Injection ◽  
Blood Injection ◽  
Quantitative Neuroimaging ◽  
The Brain

Iron released after intracerebral hemorrhage (ICH) is damaging to the brain. Measurement of the content and distribution of iron in the hematoma could predict brain damage. In this study, 16 Yorkshire piglets were subjected to autologous blood injection ICH model and studied longitudinally using quantitative susceptibility mapping and R2* relaxivity MRI on day 1 and 7 post-ICH. Phantom calibration of susceptibility demonstrated (1) iron distribution heterogeneity within the hematoma and (2) natural absorption of iron from 154 ± 78 µg/mL (day 1) to 127 ± 33 µg/mL (day 7). R2* in the hematoma decreased at day 7. This method could be adopted for ICH in humans.

scholarly journals Autologous Blood Injection and Wrist Immobilisation for Chronic Lateral Epicondylitis

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Nicola Massy-Westropp ◽  
Stuart Simmonds ◽  
Suzanne Caragianis ◽  
Andrew Potter
Keyword(s):  
Tennis Elbow ◽  
Autologous Blood ◽  
Lateral Epicondylitis ◽  
Contralateral Limb ◽  
Cortisone Injection ◽  
Autologous Blood Injection ◽  
Blood Injection ◽  
And Function ◽  
Prospective Longitudinal ◽  
Extensor Carpi Radialis

Purpose. This study explored the effect of autologous blood injection (with ultrasound guidance) to the elbows of patients who had radiologically assessed degeneration of the origin of extensor carpi radialis brevis and failed cortisone injection/s to the lateral epicondylitis.Methods. This prospective longitudinal series involved preinjection assessment of pain, grip strength, and function, using the patient-rated tennis elbow evaluation. Patients were injected with blood from the contralateral limb and then wore a customised wrist support for five days, after which they commenced a stretching, strengthening, and massage programme with an occupational therapist. These patients were assessed after six months and then finally between 18 months and five years after injection, using the patient-rated tennis elbow evaluation.Results. Thirty-eight of 40 patients completed the study, showing significant improvement in pain; the worst pain decreased by two to five points out of a 10-point visual analogue for pain. Self-perceived function improved by 11–25 points out of 100. Women showed significant increase in grip, but men did not.Conclusions. Autologous blood injection improved pain and function in a worker’s compensation cohort of patients with chronic lateral epicondylitis, who had not had relief with cortisone injection.

Long-term success with autologous blood injection for leaking trabeculectomy blebs

2010 ◽  
Vol 94 (3) ◽  
pp. 392-393 ◽  
Author(s):  
C. Dinah ◽  
B. Bhachech ◽  
G. Ghosh
Keyword(s):  
Autologous Blood ◽  
Autologous Blood Injection ◽  
Blood Injection

Autologous blood injection for late-onset filtering bleb leak

2001 ◽  
Vol 132 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Alan Burnstein ◽  
Darrell WuDunn ◽  
Yoko Ishii ◽  
Christian Jonescu-Cuypers ◽  
Louis B Cantor
Keyword(s):  
Late Onset ◽  
Autologous Blood ◽  
Filtering Bleb ◽  
Autologous Blood Injection ◽  
Blood Injection ◽  
Bleb Leak

Corneal Blood Staining Following Autologous Blood Injection for Hypotony Maculopathy

1997 ◽  
Vol 28 (10) ◽  
pp. 866-868
Author(s):  
Ramesh S Ayyala ◽  
Robert C Urban ◽  
Mina S Krishnamurthy ◽  
David J Mendelblatt
Keyword(s):  
Autologous Blood ◽  
Hypotony Maculopathy ◽  
Autologous Blood Injection ◽  
Blood Injection

Abstract WMP105: Human Monocyte Derived Macrophage Phagocytize Eryptotic Erythrocytes in a Phosphotidylserine Dependent Mechanism

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Arthur F Steinschneider ◽  
Che-Feng Chang ◽  
Michael H Askenase ◽  
Youxi Ai ◽  
Lauren H Sansing
Keyword(s):  
Autologous Blood ◽  
Annexin V ◽  
Human Monocyte ◽  
Human Macrophages ◽  
Receptor Interactions ◽  
Autologous Blood Injection ◽  
Blood Injection ◽  
Fresh Rbcs ◽  
Dependent Mechanism

Introduction: After intracerebral hemorrhage (ICH), erythrocytes contribute to secondary injury by releasing toxic hemoproteins. Our lab has previously shown that blood derived macrophages play an important role in ICH clearance but mechanisms of phagocytosis by human macrophages are unknown. This study aims to quantify eryptotic (phosphatidylserine (PtdSer)-expressing) red blood cells (RBCs) in an in vivo model of ICH, and to investigate the mechanisms that play a role in autologous eryptotic phagocytosis by human monocyte derived macrophages (huMDMs). Methods: ICH was induced in mice by autologous blood injection. The mice were sacrificed at 1 day after ICH. The brains were separated into hemispheres and digested into a single cell suspension for analysis by flow cytometry. Cells were stained with antibodies to cell surface markers and annexin V to quantify externalized PtdSer expression. Human monocytes were cultured with M-CSF for 7 days to generate huMDMs. Autologous RBCs were heat shocked (HS) to induce eryptosis. The huMDMs were cocultured with HS RBCs, HS RBCs treated with annexin V, or control RBCs. After 1 hour of coculture, the huMDMs were washed, stained and erythrophagocytosis quantified by microscopy. Results: The proportion of cells that externalized PtdSer increased by almost 20 fold at day 1 after ICH. Control brains mixed with fresh RBCs and subjected to tissue prep did not show PtdSer expression, ensuring that the PtdSer expression detected was induced in vivo (Fig A). HS RBCs increased PtdSer expression and were efficiently phagocytosed by huMDMs. Treatment of HS RBCs with annexin V to antagonize PtdSer-receptor interactions decreased RBC phagocytosis to levels comparable to control RBCs (Figs B and C). Conclusions: In vivo after ICH, erythrocytes externalize PtdSer, a cue to be engulfed by macrophages. Human macrophages phagocytose RBCs in a PtdSer-dependent mechanism. These findings highlight potential targets to enhance ICH clearance.

scholarly journals The Effect of Chronic Temporomandibular Joint Dislocation: Frequency on the Success of Autologous Blood Injection

2012 ◽  
Vol 12 (4) ◽  
pp. 414-417 ◽  
Author(s):  
Celal Candirli ◽  
Yavuz Tolga Korkmaz ◽  
Serdar Yuce ◽  
Ezher Hamza Dayisoylu ◽  
Fatih Taskesen
Keyword(s):  
Temporomandibular Joint ◽  
Autologous Blood ◽  
Joint Dislocation ◽  
Autologous Blood Injection ◽  
Blood Injection ◽  
Temporomandibular Joint Dislocation

Delayed Hyphema and Intravitreal Blood Following Intrableb Autologous Blood Injection After Trabeculectomy

1997 ◽  
Vol 124 (1) ◽  
pp. 115-116 ◽  
Author(s):  
William J. Flynn ◽  
William J. Rosen ◽  
David G. Campbell
Keyword(s):  
Autologous Blood ◽  
Autologous Blood Injection ◽  
Blood Injection
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