scholarly journals Stereotactic Intracranial Implantation and In vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme

10.3791/4089 ◽  
2012 ◽  
Author(s):  
Brian C. Baumann ◽  
Jay F. Dorsey ◽  
Joseph L. Benci ◽  
Daniel Y. Joh ◽  
Gary D. Kao
Keyword(s):  
Glioblastoma Multiforme ◽  
Mouse Model ◽  
Model System ◽  
Bioluminescent Imaging ◽  
Tumor Xenografts ◽  
Mouse Model System

Toward distinguishing LSCs from HSCs

Blood ◽  
2007 ◽  
Vol 110 (7) ◽  
pp. 2222-2223
Author(s):  
Xiaoyan Jiang
Keyword(s):  
Mouse Model ◽  
Model System ◽  
Mouse Model System

LSCs make up a critical, but very rare, population that is very difficult to study in vivo. Neering and colleagues developed a unique mouse model system in which the properties of human LSCs are closely mimicked during CML development.

P02-27 Challenges encountered in the development of an in vivo mouse model system for testing growth hormone antagonist molecules

2012 ◽  
Vol 22 ◽  
pp. S63
Author(s):  
M. Bielohuby ◽  
S. Pradhananga ◽  
I.R. Wilkinson ◽  
J.R. Sayers ◽  
P.J. Artymiuk ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Mouse Model ◽  
Model System ◽  
Mouse Model System

scholarly journals EXPRESSION OF E8^E2 IS REQUIRED FOR WART FORMATION BY MOUSE PAPILLOMAVIRUS 1 IN VIVO

2021 ◽  
Author(s):  
Frank Stubenrauch ◽  
Elke Straub ◽  
Katrin Klein ◽  
Daniela Kramer ◽  
Thomas Iftner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mouse Model ◽  
Mus Musculus ◽  
Viral Gene Expression ◽  
Model System ◽  
Viral Gene ◽  
Genome Replication ◽  
E2 Protein ◽  
Mouse Model System

Human papillomavirus (HPV) E1 and E2 proteins activate genome replication. E2 also modulates viral gene expression and is involved in the segregation of viral genomes. In addition to full length E2, almost all PV share the ability to encode an E8^E2 protein, that is a fusion of E8 with the C-terminal half of E2 which mediates specific DNA-binding and dimerization. HPV E8^E2 acts as a repressor of viral gene expression and genome replication. To analyze the function of E8^E2 in vivo, we used the Mus musculus PV1 (MmuPV1)-mouse model system. Characterization of the MmuPV1 E8^E2 protein revealed that it inhibits transcription from viral promoters in the absence and presence of E1 and E2 proteins and that this is partially dependent upon the E8 domain. MmuPV1 genomes, in which the E8 ATG start codon was disrupted (E8-), displayed a 10- to 25-fold increase in viral gene expression compared to wt genomes in cultured normal mouse tail keratinocytes in short-term experiments. This suggests that the function and mechanism of E8^E2 is conserved between MmuPV1 and HPVs. Surprisingly, challenge of athymic nude Foxn1nu/nu mice with MmuPV1 E8- genomes did not induce warts on the tail in contrast to wt MmuPV1. Furthermore, viral gene expression was completely absent at E8- MmuPV1 sites 20 - 22 weeks after DNA challenge on the tail or quasivirus challenge in the vaginal vault. This reveals that expression of E8^E2 is necessary to form tumors in vivo and that this is independent from the presence of T-cells. IMPORTANCE HPV encode an E8^E2 protein which acts as repressors of viral gene expression and genome replication. In cultured normal keratinocytes, E8^E2 is essential for long-term episomal maintenance of HPV31 genomes, but not for HPV16. To understand E8^E2's role in vivo, the Mus musculus PV1 (MmuPV1)-mouse model system was used. This revealed that E8^E2's function as a repressor of viral gene expression is conserved. Surprisingly, MmuPV1 E8^E2 knock out genomes did not induce warts in T-cell deficient mice. This shows for the first time that expression of E8^E2 is necessary for tumor formation in vivo independently of T cell immunity. This indicates that E8^E2 could be an interesting target for anti-viral therapy in vivo.

A combination of 5-fluorouracil and membrane-bound antibody inhibits B-cell lymphoma growth in a mouse model system

2007 ◽  
Vol 48 (2) ◽  
pp. 406-409 ◽  
Author(s):  
Mattia Bonsignori ◽  
Sybil D'Costa ◽  
Sherri Surman ◽  
Julia L. Hurwitz
Keyword(s):  
Mouse Model ◽  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Model System ◽  
Membrane Bound ◽  
Mouse Model System
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