scholarly journals In vivo Neuronal Calcium Imaging in C. elegans

10.3791/50357 ◽  
2013 ◽  
Author(s):  
Samuel H. Chung ◽  
Lin Sun ◽  
Christopher V. Gabel
Keyword(s):  
Calcium Imaging ◽  
C Elegans

scholarly journals Wedge prism approach for simultaneous multichannel microscopy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hanna Cai ◽  
Yao L. Wang ◽  
Richard T. Wainner ◽  
Nicusor V. Iftimia ◽  
Christopher V. Gabel ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
Axial Direction ◽  
C Elegans ◽  
Non Invasive ◽  
Image Postprocessing ◽  
Spread Function ◽  
Wedge Prism ◽  
User Friendly ◽  
Biology Research

AbstractMultichannel (multicolor) imaging has become a powerful technique in biology research for performing in vivo neuronal calcium imaging, colocalization of fluorescent labels, non-invasive pH measurement, and other procedures. We describe a novel add-on approach for simultaneous multichannel optical microscopy based on simple wedge prisms. Our device requires no alignment and is simple, robust, user-friendly, and less expensive than current commercial instruments based on switchable filters or dual-view strategies. Point spread function measurements and simulations in Zemax indicate a reduction in resolution in the direction orthogonal to the wedge interface and in the axial direction, without introducing aberration. These effects depend on the objective utilized and are most significant near the periphery of the field of view. We tested a two-channel device on C. elegans neurons in vivo and demonstrated comparable signals to a conventional dual-view instrument. We also tested a four-channel device on fixed chick embryo Brainbow samples and identified individual neurons by their spectra without extensive image postprocessing. Therefore, we believe that this technology has the potential for broad use in microscopy.

In Vivo Calcium Imaging in C. elegans Body Wall Muscles

10.3791/59175 ◽  
2019 ◽  
Author(s):  
Ashley A. Martin ◽  
Simon Alford ◽  
Janet E. Richmond
Keyword(s):  
Calcium Imaging ◽  
Body Wall ◽  
C Elegans ◽  
Body Wall Muscles ◽  
In Vivo Calcium Imaging

scholarly journals In vivo calcium imaging of OFF-responding ASK chemosensory neurons in C. elegans

2009 ◽  
Vol 1790 (8) ◽  
pp. 765-769 ◽  
Author(s):  
Tokumitsu Wakabayashi ◽  
Yukihiro Kimura ◽  
Yusuke Ohba ◽  
Ryota Adachi ◽  
Yoh-ichi Satoh ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
Chemosensory Neurons ◽  
C Elegans ◽  
In Vivo Calcium Imaging

scholarly journals In vivo calcium imaging of OFF-responding ASK chemosensory neurons in C. elegans

2009 ◽  
Vol 65 ◽  
pp. S174-S175
Author(s):  
Yusuke Ohba ◽  
Tokumitsu Wakabayashi ◽  
Yukihiro Kimura ◽  
Yoh-ichi Satoh ◽  
Ryuzo Shingai
Keyword(s):  
Calcium Imaging ◽  
Chemosensory Neurons ◽  
C Elegans ◽  
In Vivo Calcium Imaging

In silico and In vivo Evaluation of Oxidative Stress Inhibitors Against Parkinson`s Disease using the C. elegans Model

2020 ◽  
Vol 23 (8) ◽  
pp. 814-826
Author(s):  
Pradeep Hanumanthappa ◽  
Arpitha Ashok ◽  
Inderjit Prakash ◽  
Carmel I. Priya ◽  
Julie Zinzala ◽  
...  
Keyword(s):  
Neuronal Death ◽  
Neurodegenerative Disorder ◽  
In Vivo Evaluation ◽  
Neuroprotective Effects ◽  
Ample Evidence ◽  
C Elegans ◽  
Rational Drug ◽  
Cullin 3 ◽  
Anti Oxidant

Background: Parkinson’s disease ranks second, after Alzheimer’s as the major neurodegenerative disorder, for which no cure or disease-modifying therapies exist. Ample evidence indicate that PD manifests as a result of impaired anti-oxidative machinery leading to neuronal death wherein Cullin-3 has ascended as a potential therapeutic target for diseases involving damaged anti-oxidative machinery. Objective: The design of target specific inhibitors for the Cullin-3 protein might be a promising strategy to increase the Nrf2 levels and to decrease the possibility of “off-target” toxic properties. Methods: In the present study, an integrated computational and wet lab approach was adopted to identify small molecule inhibitors for Cullin-3. The rational drug designing process comprised homology modeling and derivation of the pharmacophore for Cullin-3, virtual screening of Zinc natural compound database, molecular docking and Molecular dynamics based screening of ligand molecules. In vivo validations of an identified lead compound were conducted in the PD model of C. elegans. Results and Discussion: Our strategy yielded a potential inhibitor; (Glide score = -12.31), which was evaluated for its neuroprotective efficacy in the PD model of C. elegans. The inhibitor was able to efficiently defend against neuronal death in PD model of C. elegans and the neuroprotective effects were attributed to its anti-oxidant activities, supported by the increase in superoxide dismutase, catalase and the diminution of acetylcholinesterase and reactive oxygen species levels. In addition, the Cullin-3 inhibitor significantly restored the behavioral deficits in the transgenic C. elegans. Conclusion: Taken together, these findings highlight the potential utility of Cullin-3 inhibition to block the persistent neuronal death in PD. Further studies focusing on Cullin-3 and its mechanism of action would be interesting.

Delivery Efficacy Differences of Intravenous and Intraperitoneal Injection of Exosomes: Perspectives from Tracking Dye Labeled and MiRNA Encapsulated Exosomes

2020 ◽  
Vol 17 (3) ◽  
pp. 186-194 ◽  
Author(s):  
Xueying Zhou ◽  
Zhelong Li ◽  
Wenqi Sun ◽  
Guodong Yang ◽  
Changyang Xing ◽  
...  
Keyword(s):  
Intraperitoneal Injection ◽  
Drug Delivery Vehicles ◽  
C Elegans ◽  
Administration Routes ◽  
In Vivo Distribution ◽  
Obesity Therapy ◽  
Delivery Vehicles ◽  
Visceral Adipose ◽  
Mirna Abundance

Background: Exosomes are cell-derived nanovesicles that play vital roles in intercellular communication. Recently, exosomes are recognized as promising drug delivery vehicles. Up till now, how the in vivo distribution of exosomes is affected by different administration routes has not been fully understood. Methods: In the present study, in vivo distribution of exosomes following intravenous and intraperitoneal injection approaches was systemically analyzed by tracking the fluorescence-labeled exosomes and qPCR analysis of C. elegans specific miRNA abundance delivered by exosomes in different organs. Results: The results showed that exosomes administered through tail vein were mostly taken up by the liver, spleen and lungs while exosomes injected intraperitoneally were more dispersedly distributed. Besides the liver, spleen, and lungs, intraperitoneal injection effectively delivered exosomes into the visceral adipose tissue, making it a promising strategy for obesity therapy. Moreover, the results from fluorescence tracking and qPCR were slightly different, which could be explained by systemic errors. Conclusion: Together, our study reveals that different administration routes cause a significant differential in vivo distribution of exosomes, suggesting that optimization of the delivery route is prerequisite to obtain rational delivery efficiency in detailed organs.

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