scholarly journals Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

10.3791/52311 ◽  
2015 ◽  
Author(s):  
Vanessa Cabra ◽  
Montserrat Samsó
Keyword(s):  
Electron Microscopy ◽  
Data Collection ◽  
Sample Preparation ◽  
3D Reconstruction ◽  
Quality Data ◽  
High Quality ◽  
High Quality Data ◽  
Cryo Electron Microscopy

Collecting High-Quality Data

2018 ◽  
Author(s):  
Mary Kay Gugerty ◽  
Dean Karlan
Keyword(s):  
Data Collection ◽  
Social Desirability ◽  
Measurement Errors ◽  
Monitoring And Evaluation ◽  
Quality Data ◽  
Social Desirability Bias ◽  
High Quality ◽  
High Quality Data ◽  
Evaluation Systems ◽  
Demand Effect

Without high-quality data, even the best-designed monitoring and evaluation systems will collapse. Chapter 7 introduces some the basics of collecting high-quality data and discusses how to address challenges that frequently arise. High-quality data must be clearly defined and have an indicator that validly and reliably measures the intended concept. The chapter then explains how to avoid common biases and measurement errors like anchoring, social desirability bias, the experimenter demand effect, unclear wording, long recall periods, and translation context. It then guides organizations on how to find indicators, test data collection instruments, manage surveys, and train staff appropriately for data collection and entry.

scholarly journals Information Explanatory Applications in Street Activity Security – A Survey

2020 ◽  
pp. 81-83
Author(s):  
Samsudeen S ◽  
Salomi M
Keyword(s):  
Data Collection ◽  
Path Selection ◽  
Data Sources ◽  
Quality Data ◽  
High Quality ◽  
High Quality Data ◽  
Start Up ◽  
Improvement Strategies ◽  
Paper Survey

The paper survey helps to diminish the start-up complex of knowledge assortment and clear analytics for factual modeling & course improvement for probability connected by engine vehicles. We tend to seem that the writing is isolated into 2 totally different inquire concerning areas: (a) discerning/illustrative methods which endeavor in order to urge it and assess clatter hazard supported distinctive powerful conditions, and (b) improvement strategies which center by minimizing clatter probability by route, path-selection and break design. Interpretation based on inquire concerning results of the 2 streams are restricted to beat the problem that tends to show freely accessible high-quality data sources (diverse take into account plans, result factors, and indicator factors) and communicative instructive strategies (information summarization, visualization, and measuring decrease) which are used for understanding safer-routing and provides code to encourage data collection/exploration by practitioners/res

scholarly journals Amorphous nickel titanium alloy film: a new choice for cryo electron microscopy sample preparation

2020 ◽  
Author(s):  
Xiaojun Huang ◽  
Lei Zhang ◽  
Zuoling Wen ◽  
Hui Chen ◽  
Shuoguo Li ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Titanium Alloy ◽  
Data Collection ◽  
Sample Preparation ◽  
Particle Distribution ◽  
Carbon Film ◽  
Nickel Titanium ◽  
Pure Gold ◽  
Interface Problem ◽  
Cryo Electron Microscopy

ABSTRACTCryo-electron microscopy (cryoEM) has become one of the most important approach for structural biology. However, barriers are still there for an increase successful rate, a better resolution and improved efficiency from sample preparation, data collection to image processing. CryoEM sample preparation is one of the bottlenecks with many efforts made recently, including the optimization of supporting substrate (e.g. ultra-thin carbon, graphene, pure gold, 2d crystal of streptavidin, and affinity modification), which was aimed to solve air-water interface problem, or reduce beam induced motion (BIM), or change particle distribution in the grid hole. Here, we report another effort of developing a new supporting substrate, the amorphous nickel-titanium alloy (ANTA) film, for cryoEM sample preparation. Our investigations showed advantages of ANTA film in comparison with conventional carbon film, including superior electron conductivity and trace non-specific interaction with protein. These advantages yield less BIM and significantly improved particle distribution during cryoEM experiment of human apo-ferritn, thus resulting an improved reconstruction resolution from a reduced number of micrographs and particles. Unlike the pure gold film, the usage of the ANTA film is just same with the carbon film, compatible to conventional automatic cryoEM data collection procedure.

A fibre-based crystal mounting technique for protein cryocrystallography

2012 ◽  
Vol 45 (2) ◽  
pp. 362-366 ◽  
Author(s):  
Michihiro Sugahara
Keyword(s):  
Data Collection ◽  
Success Rate ◽  
Single Crystals ◽  
Conventional Technique ◽  
Synthetic Zeolite ◽  
Quality Data ◽  
High Quality ◽  
High Quality Data ◽  
X Ray ◽  
Loop Technique

The CryoFibre, a crystal mounting tool, has been developed for protein cryocrystallography. The technique attaches single crystals to the tips of polyester fibres, allowing removal of excess liquid around each crystal. Single-wavelength anomalous dispersion phasing using a Cu Kα X-ray source (Cu SAD) was applied to crystals from five proteins without any derivatization, demonstrating a clear improvement in the success rate of Cu SAD compared with the conventional loop technique. In addition, a xylanase crystal on the surface of a synthetic zeolite as a hetero-epitaxic nucleant was directly mounted on the CryoFibre without separation treatment of the crystal from the zeolite. The crystal had a lower mosaicity than that observed using the conventional technique, indicating that the fibre technique is suitable for high-quality data collection from zeolite-mediated crystals.

The CART Principles in More Detail

2018 ◽  
Author(s):  
Mary Kay Gugerty ◽  
Dean Karlan
Keyword(s):  
Data Collection ◽  
Theory Of Change ◽  
Quality Data ◽  
Opportunity Costs ◽  
High Quality ◽  
High Quality Data

Chapter 4 discusses the CART principles in more detail, showing how they can help organizations make difficult tradeoffs about the data they should collect. To ensure credibility, organizations should collect high-quality data and analyze them accurately. This means that all data collected must be valid, reliable, and appropriately used. Actionability requires that organizations only collect data they can commit to use. This chapter explains how the actionable principle, combined with a well-articulated theory of change, guides organizations to only collect data that will have a specific use. It then explains that, for credible data collection, organizations must ensure that the benefits of data collection outweigh the costs. All data have opportunity costs—the money and time spent collecting data could also be spent implementing programs. Finally, it explains how organizations can collect transportable data that can generate knowledge for other programs.

scholarly journals Low-cost double pendulum for high-quality data collection with open-source video tracking and analysis

HardwareX ◽  
2020 ◽  
Vol 8 ◽  
pp. e00138
Author(s):  
Audun D. Myers ◽  
Joshua R. Tempelman ◽  
David Petrushenko ◽  
Firas A. Khasawneh
Keyword(s):  
Data Collection ◽  
Open Source ◽  
Low Cost ◽  
Video Tracking ◽  
Quality Data ◽  
Double Pendulum ◽  
High Quality ◽  
High Quality Data
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