A fibre-based crystal mounting technique for protein cryocrystallography

2012 ◽  
Vol 45 (2) ◽  
pp. 362-366 ◽  
Author(s):  
Michihiro Sugahara
Keyword(s):  
Data Collection ◽  
Success Rate ◽  
Single Crystals ◽  
Conventional Technique ◽  
Synthetic Zeolite ◽  
Quality Data ◽  
High Quality ◽  
High Quality Data ◽  
X Ray ◽  
Loop Technique

The CryoFibre, a crystal mounting tool, has been developed for protein cryocrystallography. The technique attaches single crystals to the tips of polyester fibres, allowing removal of excess liquid around each crystal. Single-wavelength anomalous dispersion phasing using a Cu Kα X-ray source (Cu SAD) was applied to crystals from five proteins without any derivatization, demonstrating a clear improvement in the success rate of Cu SAD compared with the conventional loop technique. In addition, a xylanase crystal on the surface of a synthetic zeolite as a hetero-epitaxic nucleant was directly mounted on the CryoFibre without separation treatment of the crystal from the zeolite. The crystal had a lower mosaicity than that observed using the conventional technique, indicating that the fibre technique is suitable for high-quality data collection from zeolite-mediated crystals.

Collecting High-Quality Data

2018 ◽  
Author(s):  
Mary Kay Gugerty ◽  
Dean Karlan
Keyword(s):  
Data Collection ◽  
Social Desirability ◽  
Measurement Errors ◽  
Monitoring And Evaluation ◽  
Quality Data ◽  
Social Desirability Bias ◽  
High Quality ◽  
High Quality Data ◽  
Evaluation Systems ◽  
Demand Effect

Without high-quality data, even the best-designed monitoring and evaluation systems will collapse. Chapter 7 introduces some the basics of collecting high-quality data and discusses how to address challenges that frequently arise. High-quality data must be clearly defined and have an indicator that validly and reliably measures the intended concept. The chapter then explains how to avoid common biases and measurement errors like anchoring, social desirability bias, the experimenter demand effect, unclear wording, long recall periods, and translation context. It then guides organizations on how to find indicators, test data collection instruments, manage surveys, and train staff appropriately for data collection and entry.

scholarly journals X-ray Variability of the Flare Star CC Eri

1995 ◽  
Vol 151 ◽  
pp. 170-170
Author(s):  
H.C. Pan ◽  
C. Jordan
Keyword(s):  
Binary Star ◽  
Emission Measure ◽  
Quality Data ◽  
Spectral Variability ◽  
High Quality ◽  
High Quality Data ◽  
X Ray ◽  
Spectroscopic Binary ◽  
Two Temperature ◽  
Continuous Temperature

The flare/spotted spectroscopic binary star CC Eri (HD 16157) was observed in the periods 1990 July 9-11 and 1992 January 26-27 with the ROSAT PSPC detector (Pan and Jordan 1995). These high quality data give the first information on the temporal and spectral variability of CC Eri in the X-ray energy band.During the ROSAT observations the X-ray intensity of the source is variable on timescales from a few minutes to several hours. The X-ray luminosity is in the range 2.5 – 6.8 × 1029 erg s−1, which is similar to values found from previous measurements with the Einstein IPC and EXOSAT LE. On 1990 July 10 an X-ray flare-like event was detected with an exponential rise time of about one hour and a decay time of about two hours. The X-ray spectrum of CC Eri can be described by current thermal plasma codes with two temperature components or with a continuous temperature distribution. The spectral results show that plasma at Te ∼ 107 K exists in the corona of CC Eri. We find that the X-ray spectrum is also variable and the variations of the emission measure, and to a lesser extent of the temperature, are correlated with the source intensity.

scholarly journals Information Explanatory Applications in Street Activity Security – A Survey

2020 ◽  
pp. 81-83
Author(s):  
Samsudeen S ◽  
Salomi M
Keyword(s):  
Data Collection ◽  
Path Selection ◽  
Data Sources ◽  
Quality Data ◽  
High Quality ◽  
High Quality Data ◽  
Start Up ◽  
Improvement Strategies ◽  
Paper Survey

The paper survey helps to diminish the start-up complex of knowledge assortment and clear analytics for factual modeling & course improvement for probability connected by engine vehicles. We tend to seem that the writing is isolated into 2 totally different inquire concerning areas: (a) discerning/illustrative methods which endeavor in order to urge it and assess clatter hazard supported distinctive powerful conditions, and (b) improvement strategies which center by minimizing clatter probability by route, path-selection and break design. Interpretation based on inquire concerning results of the 2 streams are restricted to beat the problem that tends to show freely accessible high-quality data sources (diverse take into account plans, result factors, and indicator factors) and communicative instructive strategies (information summarization, visualization, and measuring decrease) which are used for understanding safer-routing and provides code to encourage data collection/exploration by practitioners/res

scholarly journals Prisma the Instruments

1993 ◽  
Vol 137 ◽  
pp. 672-674
Author(s):  
Andrew Jones
Keyword(s):  
High Resolution ◽  
Stellar Structure ◽  
Quality Data ◽  
The Sun ◽  
Calibration Point ◽  
High Quality ◽  
High Quality Data ◽  
X Ray ◽  
Very High ◽  
Selection Of

Our understanding of Solar structure has increased dramatically in the last couple of decades thanks mainly to the opening of new windows of observation providing high quality data to theoreticians with access to powerful computing facilities. Two of the new windows were UV and X-ray images of the Sun, allowing a detailed view of the upper solar atmosphere, and the development of very high resolution spectrometers allowing us to exploit the solar oscillations to probe the internal structure of the Sun. It is the goal of PRISMA to extend these techniques to other stars, which using the Sun as a calibration point will allow us to explore stellar structure and evolution in ways not possible now.In this poster I will present a possible selection of instruments able to achieve this goal, and explain some of the rationale in their design. A more general overview is presented by T. Appouchaux also in these proceedings. It must be stressed that these are not the definitive instruments to be flown on PRISMA, but rather result from a study to show the feasibility of such a mission. Should PRISMA be chosen as the next ESA medium sized mission, an ‘Announcement of Opportunity’ wiH be issued by ESA and the responses of all people interested in constructing the instrument will be considered.

The CART Principles in More Detail

2018 ◽  
Author(s):  
Mary Kay Gugerty ◽  
Dean Karlan
Keyword(s):  
Data Collection ◽  
Theory Of Change ◽  
Quality Data ◽  
Opportunity Costs ◽  
High Quality ◽  
High Quality Data

Chapter 4 discusses the CART principles in more detail, showing how they can help organizations make difficult tradeoffs about the data they should collect. To ensure credibility, organizations should collect high-quality data and analyze them accurately. This means that all data collected must be valid, reliable, and appropriately used. Actionability requires that organizations only collect data they can commit to use. This chapter explains how the actionable principle, combined with a well-articulated theory of change, guides organizations to only collect data that will have a specific use. It then explains that, for credible data collection, organizations must ensure that the benefits of data collection outweigh the costs. All data have opportunity costs—the money and time spent collecting data could also be spent implementing programs. Finally, it explains how organizations can collect transportable data that can generate knowledge for other programs.

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