Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Journal of Visualized Experiments ◽

10.3791/58012 ◽

2018 ◽

Author(s):

Keri L Colabroy ◽

Katlyn Mayer

Keyword(s):

Affinity Chromatography ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity ◽

Undergraduate Laboratory

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Faculty Opinions recommendation of Evaluating immobilized metal affinity chromatography for the selection of histidine-containing peptides in comparative proteomics.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1014119.192808 ◽

2003 ◽

Author(s):

George Janini

Keyword(s):

Affinity Chromatography ◽

Comparative Proteomics ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity ◽

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Faculty Opinions recommendation of Immobilized metal affinity chromatography as a drug discovery platform for metalloenzyme inhibitors.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.738685288.793578992 ◽

2020 ◽

Author(s):

Seth Cohen

Keyword(s):

Drug Discovery ◽

Affinity Chromatography ◽

Immobilized Metal Affinity Chromatography ◽

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Characterization of mercury-binding proteins in human neuroblastoma SK-N-SH cells with immobilized metal affinity chromatography

Talanta ◽

10.1016/j.talanta.2017.10.028 ◽

2018 ◽

Vol 178 ◽

pp. 811-817 ◽

Author(s):

Yiling Li ◽

Bin He ◽

Ligang Hu ◽

Xiu Huang ◽

Zhaojun Yun ◽

...

Keyword(s):

Affinity Chromatography ◽

Binding Proteins ◽

Immobilized Metal Affinity Chromatography ◽

Human Neuroblastoma ◽

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Studies with an immobilized metal affinity chromatography cassette system involving binuclear triazacyclononane-derived ligands: Automation of batch adsorption measurements with tagged recombinant proteins

Journal of Chromatography A ◽

10.1016/j.chroma.2014.05.030 ◽

2014 ◽

Vol 1351 ◽

pp. 61-69 ◽

Author(s):

Martin Petzold ◽

Campbell J. Coghlan ◽

Milton T.W. Hearn

Keyword(s):

Affinity Chromatography ◽

Recombinant Proteins ◽

Immobilized Metal Affinity Chromatography ◽

Batch Adsorption ◽

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Construction of a modified penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus and purification by immobilized metal affinity chromatography.

Journal of Bacteriology ◽

10.1128/jb.176.5.1539-1541.1994 ◽

1994 ◽

Vol 176 (5) ◽

pp. 1539-1541 ◽

Author(s):

C Y Wu ◽

L C Blaszczak ◽

M C Smith ◽

P L Skatrud

Keyword(s):

Staphylococcus Aureus ◽

Affinity Chromatography ◽

Methicillin Resistant Staphylococcus Aureus ◽

Binding Protein ◽

Immobilized Metal Affinity Chromatography ◽

Penicillin Binding Protein ◽

Methicillin Resistant ◽

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Separation of jack bean (Canavalia ensiformis) urease isoforms by immobilized metal affinity chromatography and characterization of insecticidal properties unrelated to ureolytic activity

Plant Science ◽

10.1016/s0168-9452(04)00142-6 ◽

2004 ◽

Author(s):

C FOLLMER

Keyword(s):

Affinity Chromatography ◽

Immobilized Metal Affinity Chromatography ◽

Jack Bean ◽

Canavalia Ensiformis ◽

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Immobilized Metal Affinity Chromatography

Encyclopedia of Chromatography ◽

10.1201/noe0824727857-174 ◽

2005 ◽

pp. 819-821

Author(s):

Roy A. Musil

Keyword(s):

Affinity Chromatography ◽

Immobilized Metal Affinity Chromatography ◽

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Effects of size, buffer composition, number and position of tag on purity and yield of purification of histidine‐tagged proteins by immobilized metal affinity chromatography

The FASEB Journal ◽

10.1096/fasebj.27.1_supplement.790.9 ◽

2013 ◽

Vol 27 (S1) ◽

Author(s):

Mireya N. Martinez ◽

Odutayo O Odunuga

Keyword(s):

Affinity Chromatography ◽

Immobilized Metal Affinity Chromatography ◽

Metal Affinity ◽

Buffer Composition

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Two-step Immobilized Metal Affinity Chromatography (IMAC) for Phosphoproteomics Using Mass Spectrometry

Journal of the Chinese Chemical Society ◽

10.1002/jccs.200500107 ◽

2005 ◽

Vol 52 (4) ◽

pp. 765-772 ◽

Author(s):

Sheng-Yu Huang ◽

Jue-Liang Hsu ◽

Shu-Hui Chen

Keyword(s):

Mass Spectrometry ◽

Affinity Chromatography ◽

Immobilized Metal Affinity Chromatography ◽

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Purification by immobilized metal affinity chromatography of human atrial natriuretic peptide expressed in a novel thioredoxin fusion protein

Biotechnology Progress ◽

10.1021/bp00033a004 ◽

1995 ◽

Vol 11 (3) ◽

pp. 265-269 ◽

Author(s):

David L. Wilkinson ◽

Nien-Tung Ma ◽

Chris Haught ◽

Roger G. Harrison

Keyword(s):

Atrial Natriuretic Peptide ◽

Fusion Protein ◽

Affinity Chromatography ◽

Natriuretic Peptide ◽

Immobilized Metal Affinity Chromatography ◽

Human Atrial Natriuretic Peptide ◽

Metal Affinity ◽

Atrial Natriuretic

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