Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester

10.3791/59545 ◽  
2019 ◽  
Author(s):  
Anthony R. Di Carluccio ◽  
Eleonora Tresoldi ◽  
Michelle So ◽  
Stuart I. Mannering
Keyword(s):  
T Cells ◽  
Succinimidyl Ester ◽  
Carboxyfluorescein Succinimidyl Ester

scholarly journals A flow cytometric assay for simultaneously measuring the proliferation and cytotoxicity of cytokine induced killer cells in combination with carboxyfluorescein succinimidyl ester (CFSE) labeling

2011 ◽  
Vol 10 (65) ◽  
pp. 14598-14607 ◽  
Author(s):  
Duan Xiu Mei ◽  
Huang Jing ◽  
Ma Hong Xi ◽  
Wang Xiao Qi ◽  
Che Yuan Yuan ◽  
...  
Keyword(s):  
Flow Cytometric Assay ◽  
Killer Cells ◽  
Flow Cytometric ◽  
Succinimidyl Ester ◽  
Carboxyfluorescein Succinimidyl Ester

scholarly journals “Pruning” of Alloreactive CD4+T Cells Using 5- (and 6-)Carboxyfluorescein Diacetate Succinimidyl Ester Prolongs Skin Allograft Survival

2004 ◽  
Vol 173 (11) ◽  
pp. 6574-6582 ◽  
Author(s):  
Debbie Watson ◽  
Geoff Yu Zhang ◽  
Mary Sartor ◽  
Stephen I. Alexander
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Allograft Survival ◽  
Skin Allograft ◽  
Succinimidyl Ester ◽  
Diacetate Succinimidyl

scholarly journals Optimization of a highly standardized carboxyfluorescein succinimidyl ester flow cytometry panel and gating strategy design using discriminative information measure evaluation

2010 ◽  
Vol 77A (12) ◽  
pp. 1126-1136 ◽  
Author(s):  
Cliburn Chan ◽  
Lin Lin ◽  
Jacob Frelinger ◽  
Valérie Hérbert ◽  
Dominic Gagnon ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Information Measure ◽  
Succinimidyl Ester ◽  
Strategy Design ◽  
Measure Evaluation ◽  
Carboxyfluorescein Succinimidyl Ester

Nanomolar derivatizations with 5-carboxyfluorescein succinimidyl ester for fluorescence detection in capillary electrophoresis

1998 ◽  
Vol 809 (1-2) ◽  
pp. 203-210 ◽  
Author(s):  
Shung Kei Lau ◽  
Fabian Zaccardo ◽  
Michael Little ◽  
Peter Banks
Keyword(s):  
Capillary Electrophoresis ◽  
Fluorescence Detection ◽  
Succinimidyl Ester ◽  
Carboxyfluorescein Succinimidyl Ester

scholarly journals Cd4+ T Cell Division in Irradiated Mice Requires Peptides Distinct from Those Responsible for Thymic Selection

1999 ◽  
Vol 190 (3) ◽  
pp. 367-374 ◽  
Author(s):  
Jeremy Bender ◽  
Tom Mitchell ◽  
John Kappler ◽  
Philippa Marrack
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cd4 T Cells ◽  
Class Ii ◽  
Cd4 T Cell ◽  
Thymic Selection ◽  
Cell Numbers ◽  
Succinimidyl Ester ◽  
Diacetate Succinimidyl ◽  
Sublethal Irradiation

We investigated the mechanism by which α/β T cells expand upon transfer to T cell–deficient host mice by injecting carboxyfluorescein diacetate succinimidyl ester–labeled T cells into mice depleted of T cells by sublethal irradiation. We found that CD4+ T cells divided when transferred to irradiated hosts and that the division of more than half of these cells required class II expression. However, division of transferred CD4+ T cells did not occur in irradiated hosts that expressed class II molecules occupied solely by the peptide responsible for thymic selection, indicating that peptides distinct from those involved in thymic selection cause the division of CD4+ T cells in irradiated mice. These data establish that class II–bound peptides control the expansion of CD4+ T cells transferred to T cell–deficient hosts and suggest that the same peptides contribute to the maintenance of T cell numbers in normal mice.

scholarly journals Storage of Erythrocytes Induces Suicidal Erythrocyte Death

2016 ◽  
Vol 39 (2) ◽  
pp. 668-676 ◽  
Author(s):  
Elisabeth Lang ◽  
Vitaly I. Pozdeev ◽  
Haifeng C. Xu ◽  
Prashant V. Shinde ◽  
Kristina Behnke ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Blood Cells ◽  
Calcium Concentration ◽  
Annexin V ◽  
Cation Channels ◽  
Cell Shrinkage ◽  
Nonselective Cation Channels ◽  
Phosphatidylserine Translocation ◽  
Succinimidyl Ester ◽  
Carboxyfluorescein Succinimidyl Ester

Background/Aims: Similar to apoptosis of nucleated cells, red blood cells (RBC) can undergo suicidal cell death - called eryptosis. It is characterized by cell shrinkage and phosphatidylserine translocation. Eryptosis is triggered by an increase of intracellular calcium concentration due to activation of nonselective cation channels. The cation channels and consequently eryptosis are inhibited by erythropoietin. Eryptotic RBC are engulfed by macrophages and thus rapidly cleared from circulating blood. In this study, we explored whether storage of RBC influences the rate of eryptosis. Methods: Flow cytometry was employed to quantify phosphatidylserine exposing erythrocytes from annexin V binding and cytosolic Ca2+ activity from Fluo-3 fluorescence. Clearance of stored murine RBC was tested by injection of carboxyfluorescein succinimidyl ester (CFSE)-labelled erythrocytes. Results: Storage for 42 days significantly increased the percentage of phosphatidylserine exposing and haemolytic erythrocytes, an effect blunted by removal of extracellular calcium. Phosphatidylserine exposure could be inhibited by addition of erythropoietin. Upon transfusion, the clearance of murine CFSE-labelled RBC from circulating blood was significantly higher following storage for 10 days when compared to 2 days of storage. Conclusion: Storage of RBC triggers eryptosis by Ca2+ and erythropoietin sensitive mechanisms.

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