Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

10.3791/60324 ◽  
2020 ◽  
Author(s):  
Jennifer Eymael ◽  
Laura Miesen ◽  
Fieke Mooren ◽  
Jitske Jansen ◽  
Jack Wetzels ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Activation ◽  
Ex Vivo ◽  
Parietal Epithelial Cell ◽  
Ex Vivo Assay

CD9 Is a Novel Target in Glomerular Diseases Typified by Parietal Epithelial Cell Activation

2020 ◽  
Vol 75 (5) ◽  
pp. 812-814
Author(s):  
Bart Smeets ◽  
Laura Miesen ◽  
Stuart J. Shankland
Keyword(s):  
Epithelial Cell ◽  
Cell Activation ◽  
Glomerular Diseases ◽  
Parietal Epithelial Cell ◽  
Novel Target

scholarly journals New Insights into Glomerular Parietal Epithelial Cell Activation and Its Signaling Pathways in Glomerular Diseases

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Hua Su ◽  
Shan Chen ◽  
Fang-Fang He ◽  
Yu-Mei Wang ◽  
Philip Bondzie ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Signaling Pathways ◽  
Membranous Nephropathy ◽  
Cell Activation ◽  
Crescentic Glomerulonephritis ◽  
Diabetic Glomerulopathy ◽  
Glomerular Diseases ◽  
The Past ◽  
Parietal Epithelial Cell ◽  
Parietal Epithelial Cells

The glomerular parietal epithelial cells (PECs) have aroused an increasing attention recently. The proliferation of PECs is the main feature of crescentic glomerulonephritis; besides that, in the past decade, PEC activation has been identified in several types of noninflammatory glomerulonephropathies, such as focal segmental glomerulosclerosis, diabetic glomerulopathy, and membranous nephropathy. The pathogenesis of PEC activation is poorly understood; however, a few studies delicately elucidate the potential mechanisms and signaling pathways implicated in these processes. In this review we will focus on the latest observations and concepts about PEC activation in glomerular diseases and the newest identified signaling pathways in PEC activation.

scholarly journals For better or worse: a niche for Notch in parietal epithelial cell activation

2013 ◽  
Vol 83 (6) ◽  
pp. 988-990 ◽  
Author(s):  
Mariya T. Sweetwyne ◽  
Katalin Susztak
Keyword(s):  
Epithelial Cell ◽  
Cell Activation ◽  
Parietal Epithelial Cell

scholarly journals Nature and Mediators of Parietal Epithelial Cell Activation in Glomerulonephritides of Human and Rat

2013 ◽  
Vol 183 (6) ◽  
pp. 1769-1778 ◽  
Author(s):  
Paola Rizzo ◽  
Norberto Perico ◽  
Elena Gagliardini ◽  
Rubina Novelli ◽  
Malcolm R. Alison ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Activation ◽  
Parietal Epithelial Cell

scholarly journals Parietal Epithelial Cell Activation Marker in Early Recurrence of FSGS in the Transplant

2012 ◽  
Vol 7 (11) ◽  
pp. 1852-1858 ◽  
Author(s):  
Huma Fatima ◽  
Marcus J. Moeller ◽  
Bart Smeets ◽  
Hai-Chun Yang ◽  
Vivette D. D’Agati ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Activation ◽  
Early Recurrence ◽  
Activation Marker ◽  
Cell Activation Marker ◽  
Parietal Epithelial Cell

scholarly journals Endothelial Epas1 Deficiency Is Sufficient To Promote Parietal Epithelial Cell Activation and FSGS in Experimental Hypertension

2017 ◽  
Vol 28 (12) ◽  
pp. 3563-3578 ◽  
Author(s):  
Yosu Luque ◽  
Olivia Lenoir ◽  
Philippe Bonnin ◽  
Lise Hardy ◽  
Anna Chipont ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Activation ◽  
Experimental Hypertension ◽  
Parietal Epithelial Cell

scholarly journals Glomerular parietal epithelial cell activation induces collagen secretion and thickening of Bowman’s capsule in diabetes

2014 ◽  
Vol 95 (3) ◽  
pp. 273-282 ◽  
Author(s):  
Alexander Holderied ◽  
Simone Romoli ◽  
Jonathan Eberhard ◽  
Lukas A Konrad ◽  
Satish K Devarapu ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Activation ◽  
Collagen Secretion ◽  
Parietal Epithelial Cell ◽  
Bowman's Capsule

A Comparative Ex Vivo Study of Plasminogen Activators and Proteases for Fibrinolytic Activity and Side Effects in Rabbits

1977 ◽  
Vol 37 (01) ◽  
pp. 154-161 ◽  
Author(s):  
B. A Janik ◽  
S. E Papaioannou
Keyword(s):  
Side Effects ◽  
Effective Dose ◽  
Fibrinolytic Activity ◽  
Ex Vivo ◽  
Plasminogen Activators ◽  
Assay System ◽  
Coagulation Parameters ◽  
Ex Vivo Assay

SummaryUrokinase, streptokinase, Brinase, trypsin, and SN 687, a bacterial exoprotease, have been evaluated in an ex vivo assay system. These enzymes were injected into rabbits and the fibrinolytic activity as well as other coagulation parameters were measured by in vitro techniques. Dose-response correlations have been made using the euglobulin lysis time as a measure of fibrinolytic activity and the 50% effective dose has been determined for each enzyme. Loading doses, equal to four times the 50% effective dose, were administered to monitor potential toxicity revealing that Brinase, trypsin, and SN 687 were very toxic at this concentration.Having established the 50% effective dose for each enzyme, further testing was conducted where relevant fibrinolytic and coagulation parameters were measured for up to two days following a 50% effective dose bolus injection of each enzyme. Our results have demonstrated that urokinase and streptokinase are plasminogen activators specifically activating the rabbit fibrinolytic system while Brinase, trypsin and SN 687 increase the general proteolytic activity in vivo.The advantages of this ex vivo assay system for evaluating relative fibrinolytic potencies and side effects for plasminogen activators and fibrinolytic proteases have been discussed.

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