scholarly journals Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

10.3791/60726 ◽  
2020 ◽  
Author(s):  
Eileen F. Ablondi ◽  
Sudip Paudel ◽  
Morgan Sehdev ◽  
John P. Marken ◽  
Andrew D. Halleran ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Xenopus Laevis ◽  
Calcium Imaging ◽  
Neuronal Precursor

scholarly journals UNDER-REPLICATION OF RIBOSOMAL CISTRONS IN POLYTENE CHROMOSOMES OF RHYNCHOSCIARA

1974 ◽  
Vol 62 (1) ◽  
pp. 215-222 ◽  
Author(s):  
A. G. Gambarini ◽  
F. J. S. Lara
Keyword(s):  
Salivary Gland ◽  
In Situ Hybridization ◽  
Xenopus Laevis ◽  
Related Species ◽  
Polytene Chromosomes ◽  
Chromosome X ◽  
Heterologous Hybridization ◽  
Ribosomal Cistrons ◽  
Rhynchosciara Americana

DNA preparations obtained from several tissues of Rhynchosciara americana and two related species, R. milleri and R. papaveroi, were hybridized to R. americana rRNA. The percentage of hybridization was found to be higher in tissues with low polyteny than in tissues with high polyteny, suggesting a relationship between the amount of rDNA and the tissue polyteny. This could be explained by under-replication of ribosomal cistrons in polytene cells, such as those from the salivary gland. Only slight tissue-dependent changes in the percentages of hybridization can be observed in heterologous hybridization using Xenopus laevis rRNA. The possibility that these experiments could not detect differences in the amount of ribosomal cistrons among tissues is discussed. The female:male ratio for the percentages of hybridization in the salivary gland of R. americana agrees with the results obtained by in situ hybridization experiments (16, 17) which have shown that the rRNA cistrons are distributed among chromosomes other than chromosome X.

Immunocytochemistry and in situ hybridization of neuropeptide Y in the hypothalamus of Xenopus laevis in relation to background adaptation

Neuroscience ◽  
1993 ◽  
Vol 55 (3) ◽  
pp. 667-675 ◽  
Author(s):  
R. Tuinhof ◽  
F.Y.S.C. Laurent ◽  
R.G.E. Ebbers ◽  
W.J.A.J. Smeets ◽  
M.C.H.M. van Riel ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Xenopus Laevis ◽  
Neuropeptide Y ◽  
Background Adaptation

scholarly journals Shifting into high gear: how interstitial cells of Cajal change the motility pattern of the developing intestine

2020 ◽  
Vol 319 (4) ◽  
pp. G519-G528
Author(s):  
Nicolas R. Chevalier ◽  
Yanis Ammouche ◽  
Anthony Gomis ◽  
Clémence Teyssaire ◽  
Pascal de Santa Barbara ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
In Situ Hybridization ◽  
Calcium Imaging ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cell ◽  
Sharp Transition ◽  
Anoctamin 1 ◽  
Cells Of Cajal ◽  
First Time

We reveal a sharp transition from smooth muscle to interstitial cell of Cajal (ICC)-driven motility in the chicken embryo, leading to higher-frequency, more rhythmic contractile waves. We predict the transition to happen between 12 and 14 embryonic wk in humans. We image for the first time the onset of ICC activity in an embryonic gut by calcium imaging. We show the first KIT and anoctamin-1 (ANO1) in situ hybridization micrographs in the embryonic chicken gut.

The c-src1 gene visualized by in situ hybridization on Xenopus laevis chromosomes

2003 ◽  
Vol 103 (1-2) ◽  
pp. 169-172 ◽  
Author(s):  
V. Krylov ◽  
J. Mácha ◽  
T. Tlapáková ◽  
M. Takáč ◽  
J. Jonák
Keyword(s):  
In Situ Hybridization ◽  
Xenopus Laevis

Accumulation, spatial distribution and partial characterization of poly(A)+RNA in the developing oocytes of Xenopus laevis

Development ◽  
1981 ◽  
Vol 66 (1) ◽  
pp. 127-140
Author(s):  
M. Wakahara
Keyword(s):  
Spatial Distribution ◽  
In Situ Hybridization ◽  
Xenopus Laevis ◽  
Binding Activity ◽  
Dnase I ◽  
Rnase A ◽  
Type Activity ◽  
Rnase T2 ◽  
Silver Grains

In situ hybridization using [3H]poly(U) was applied to developing oocytes of Xenopus laevis, which had been fixed in Bouin's solution. Tissue sections were pretreated with DNase I, annealed with [3H]poly(U) and post-treated with RNase A and TCA. After the autoradiographical processing, silver grains over the oocyte were counted. As a result of the control experiments which included RNase A, RNase T2, DNase I and Pronase E hydrolysis and Cordycepin (3'-deoxyadenosine) incubation before in situ hybridization, it was concluded that the poly(U)-binding activity detected upon the oocytes was due to the possible presence of poly(A)+RNAs. Spatial distribution of the poly(U)-binding sites changed during the development of the oocytes; in a small oocyte before the pachytene stage, silver grains developed over the nucleus, while in a larger oocyte after the diplotene the grains were concentrated over the cytoplasm. After yolk platelets were deposited in the cytoplasm, two types of poly(U)-binding activities were noted; a bound-type activity which was firmly associated with the cytoplasm, so that the positions of the silver grains were not influenced by fixation, and an unbound type which did not bind so firmly to the cytoplasm and was therefore easily influenced by inflow of fixative. The bound-type activity persisted in the cytoplasm throughout the oogenesis, but the unbound type appeared only after the vitellogenesis, especially in the yolky cytoplasm. The total poly(U)-binding activity per oocyte increased continuously with the growth of the oocyte.

Localization of specific mRNA sequences in Xenopus laevis embryos by in situ hybridization

Development ◽  
1986 ◽  
Vol 91 (1) ◽  
pp. 153-168
Author(s):  
Eva Dworkin-Rastl ◽  
Darcy B. Kelley ◽  
Mark B. Dworkin
Keyword(s):  
In Situ Hybridization ◽  
Xenopus Laevis ◽  
Lateral Plate ◽  
Tissue Specific ◽  
Maternal Rna ◽  
Specific Hybridization ◽  
Cloned Cdna ◽  
Xenopus Laevis Embryos ◽  
Specific Sequences

In situ hybridization of cloned cDNA probes to frozen sections of Xenopus laevis stage-42 tadpoles has been used to determine the tissue localization of several mRNAs. Nine out of sixteen probes tested hybridized to most or all tadpole tissues; seven probes exhibited tissue-specific hybridization. The non-tissue-specific sequences hybridized to RNA species that are also present in maternal RNA while the tissue-specific sequences hybridized to embryonic RNA species induced after gastrulation and undetectable in maternal RNA. Tissue-specific hybridization was observed with muscle (five clones), epidermis (one clone), and the nervous system (one clone). All muscle-specific sequences hybridized to somites and lateral plate muscles, but they differed in their hybridization to heart muscle.

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