A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

10.3791/61149 ◽  
2020 ◽  
Author(s):  
Esther R. Miller ◽  
Jonah O'Mara Schwartz ◽  
Grace Cox ◽  
Benjamin E. Wolfe
Keyword(s):  
Microbiome Assembly ◽  
Vegetable Fermentation

Microbiome assembly in The Gambia

2021 ◽  
Author(s):  
Matthew M. Carter ◽  
Matthew R. Olm ◽  
Erica D. Sonnenburg
Keyword(s):  
The Gambia ◽  
Microbiome Assembly

scholarly journals Extensive Horizontal Gene Transfer in Cheese-Associated Bacteria

2016 ◽  
Author(s):  
Kevin S. Bonham ◽  
Benjamin E. Wolfe ◽  
Rachel J. Dutton
Keyword(s):  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Molecular Mechanisms ◽  
Protein Coding ◽  
Associated Bacteria ◽  
The Us ◽  
Selective Forces ◽  
Genomic Regions ◽  
Microbiome Assembly ◽  
Insight Into

AbstractAcquisition of genes through horizontal gene transfer (HGT) allows microbes to rapidly gain new capabilities and adapt to new or changing environments. Identifying widespread HGT regions within multispecies microbiomes can pinpoint the molecular mechanisms that play key roles in microbiome assembly. We sought to identify horizontally transferred genes within a model microbiome, the cheese rind. Comparing 31 newly-sequenced and 134 previously sequenced bacterial isolates from cheese rinds, we identified over 200 putative horizontally transferred genomic regions containing 4,733 protein coding genes. The largest of these regions are enriched for genes involved in siderophore acquisition, and are widely distributed in cheese rinds in both Europe and the US. These results suggest that horizontal gene transfer (HGT) is prevalent in cheese rind microbiomes, and the identification of genes that are frequently transferred in a particular environment may provide insight into the selective forces shaping microbial communities.

scholarly journals Pollinators mediate floral microbial diversity and network under agrochemical disturbance

2020 ◽  
Author(s):  
Na Wei ◽  
Avery L. Russell ◽  
Abigail R. Jarrett ◽  
Tia-Lynn Ashman
Keyword(s):  
Functional Groups ◽  
Anthropogenic Disturbance ◽  
Structural Equation ◽  
Equation Modeling ◽  
Pollinator Visitation ◽  
Microbial Network ◽  
Β Diversity ◽  
The Face ◽  
Managed Ecosystems ◽  
Microbiome Assembly

AbstractHow pollinators mediate microbiome assembly in the anthosphere is a major unresolved question of theoretical and applied importance in the face of anthropogenic disturbance. We addressed this question by linking visitation of diverse pollinator functional groups (bees, wasps, flies, butterflies, beetles, true bugs and other taxa) to the key properties of floral microbiome (microbial α- and β-diversity and microbial network) under agrochemical disturbance, using a field experiment of bactericide and fungicide treatments on cultivated strawberries that differ in flower abundance. Structural equation modeling was used to link agrochemical disturbance and flower abundance to pollinator visitation to floral microbiome properties. Our results revealed that (1) pollinator visitation influenced the α- and β-diversity and network centrality of floral microbiome, with different pollinator functional groups affecting different microbiome properties; (2) flower abundance influenced floral microbiome both directly by governing the source pool of microbes and indirectly by enhancing pollinator visitation; and (3) agrochemical disturbance affected floral microbiome primarily directly by fungicide, and less so indirectly via pollinator visitation. These findings improve the mechanistic understanding of floral microbiome assembly, and may be generalizable to many other plants that are visited by diverse insect pollinators in natural and managed ecosystems.

Vegetable Fermentation and Pickling

2018 ◽  
pp. 407-427
Author(s):  
Sami Ghnimi ◽  
Nejib Guizani
Keyword(s):  
Vegetable Fermentation

scholarly journals Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota

2018 ◽  
Vol 12 (5) ◽  
pp. 1375-1388 ◽  
Author(s):  
H. Pieter J. van Veelen ◽  
Joana Falcão Salles ◽  
B. Irene Tieleman
Keyword(s):  
Microbiome Assembly
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