Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy

Journal of Visualized Experiments ◽

10.3791/62145 ◽

2021 ◽

Author(s):

Thais Bascuas ◽

Martina Kropp ◽

Nina Harmening ◽

Brittany M. Wong ◽

Sandra Johnen ◽

...

Keyword(s):

Gene Therapy ◽

Epithelial Cells ◽

Genetic Engineering ◽

Pigment Epithelial ◽

Viral Gene Therapy ◽

Pigment Epithelial Cells

Download Full-text

Optimizing Non-viral Gene Therapy Vectors for Delivery to Photoreceptors and Retinal Pigment Epithelial Cells

Retinal Degenerative Diseases - Advances in Experimental Medicine and Biology ◽

10.1007/978-3-319-75402-4_14 ◽

2018 ◽

pp. 109-115 ◽

Author(s):

Rahel Zulliger ◽

Jamie N. Watson ◽

Muayyad R. Al-Ubaidi ◽

Linas Padegimas ◽

Ozge Sesenoglu-Laird ◽

...

Keyword(s):

Gene Therapy ◽

Epithelial Cells ◽

Retinal Pigment Epithelial ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Pigment Epithelial ◽

Viral Gene Therapy ◽

Gene Therapy Vectors ◽

Pigment Epithelial Cells

Download Full-text

659. Safety of Recombinant Lentiviral Mediated Ex-Vivo Gene Therapy Using Retinal Pigment Epithelial Cells in Hemiparkinsonian Rats

Molecular Therapy ◽

10.1016/s1525-0016(16)41101-9 ◽

2003 ◽

Vol 7 (5) ◽

pp. S256

Keyword(s):

Gene Therapy ◽

Epithelial Cells ◽

Retinal Pigment Epithelial ◽

Ex Vivo ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Pigment Epithelial ◽

Ex Vivo Gene Therapy ◽

Pigment Epithelial Cells

Download Full-text

Optimization of non-viral gene transfer to human primary retinal pigment epithelial cells

Current Eye Research ◽

10.1076/0271-3683(200005)2051-1ft361 ◽

2000 ◽

Vol 20 (5) ◽

pp. 361-366 ◽

Author(s):

Khaled Abul-Hassan ◽

Richard Walmsley ◽

Mike Boulton

Keyword(s):

Gene Transfer ◽

Epithelial Cells ◽

Retinal Pigment Epithelial ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Pigment Epithelial ◽

Viral Gene Transfer ◽

Pigment Epithelial Cells

Download Full-text

Cell polarity, phagocytosis and viral gene transfer in cultured human retinal pigment epithelial cells

Current Eye Research ◽

10.1080/02713689808951241 ◽

1998 ◽

Vol 17 (6) ◽

pp. 668-672 ◽

Author(s):

Lyndon da Cruz ◽

Terry Robertson ◽

Michael O. Hall ◽

Ian J. Constable ◽

Piroska E. Rakoczy

Keyword(s):

Gene Transfer ◽

Epithelial Cells ◽

Cell Polarity ◽

Retinal Pigment Epithelial ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Pigment Epithelial ◽

Viral Gene Transfer ◽

Pigment Epithelial Cells

Download Full-text

A New Rapid and Non-radioactive Assay for Monitoring and Determining the Proliferation of Retinal Pigment Epithelial Cells

Korean Journal of Ophthalmology ◽

10.3341/kjo.2003.17.1.29 ◽

2003 ◽

Vol 17 (1) ◽

pp. 29 ◽

Author(s):

Hyeong Gon Yu ◽

Hum Chung ◽

Young Suk Yu ◽

Jong Mo Seo ◽

Jang Won Heo

Keyword(s):

Epithelial Cells ◽

Retinal Pigment Epithelial ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Pigment Epithelial ◽

Radioactive Assay ◽

Pigment Epithelial Cells

Download Full-text

Nuclear Receptor Atlas of Human Retinal Pigment Epithelial Cells: Potential Relevance to Age-Related Macular Degeneration.

NURSA Datasets ◽

10.1621/datasets.06001 ◽

2013 ◽

Author(s):

MA Dwyer ◽

D Kazmin ◽

P Hu ◽

DP McDonnell ◽

G Malek

Keyword(s):

Epithelial Cells ◽

Macular Degeneration ◽

Nuclear Receptor ◽

Retinal Pigment Epithelial ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Age Related Macular Degeneration ◽

Pigment Epithelial ◽

Age Related ◽

Pigment Epithelial Cells

Download Full-text

Eupatilin attenuates TGF-β2-induced proliferation and epithelial-mesenchymal transition of retinal pigment epithelial cells

Cutaneous and Ocular Toxicology ◽

10.1080/15569527.2021.1902343 ◽

2021 ◽

pp. 1-24

Author(s):

Ayca Kupeli Cinar ◽

S. Altan Ozal ◽

Riza Serttas ◽

Suat Erdogan

Keyword(s):

Epithelial Cells ◽

Retinal Pigment Epithelial ◽

Epithelial Mesenchymal Transition ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Mesenchymal Transition ◽

Pigment Epithelial ◽

Pigment Epithelial Cells

Download Full-text

Exendin-4 inhibits high glucose-induced oxidative stress in retinal pigment epithelial cells by modulating the expression and activation of p66Shc

Cutaneous and Ocular Toxicology ◽

10.1080/15569527.2020.1844727 ◽

2021 ◽

pp. 1-12

Author(s):

Nasser Al Sabaani

Keyword(s):

Oxidative Stress ◽

Epithelial Cells ◽

High Glucose ◽

Retinal Pigment Epithelial ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Pigment Epithelial ◽

Pigment Epithelial Cells

Download Full-text

CD63+ extracellular vesicles from retinal pigment epithelial cells participate in crosstalk with macrophages in the innate inflammatory axis

Experimental Eye Research ◽

10.1016/j.exer.2021.108496 ◽

2021 ◽

Vol 205 ◽

pp. 108496

Author(s):

Yohei Otsuki ◽

Eiko Ito ◽

Atsushi Mukai ◽

Morio Ueno ◽

Takahiro Yamawaki ◽

...

Keyword(s):

Epithelial Cells ◽

Extracellular Vesicles ◽

Retinal Pigment Epithelial ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Pigment Epithelial ◽

Pigment Epithelial Cells

Download Full-text

Uptake of retinol and retinoic acid from serum retinol-binding protein by retinal pigment epithelial cells.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)63334-x ◽

1977 ◽

Vol 252 (15) ◽

pp. 5216-5221 ◽

Author(s):

C C Chen ◽

J Heller

Keyword(s):

Retinoic Acid ◽

Epithelial Cells ◽

Retinal Pigment Epithelial ◽

Binding Protein ◽

Retinal Pigment ◽

Retinal Pigment Epithelial Cells ◽

Retinol Binding Protein ◽

Serum Retinol ◽

Pigment Epithelial ◽

Pigment Epithelial Cells

Download Full-text