Activity of Posterior Lateral Line Afferent Neurons during Swimming in Zebrafish

10.3791/62233 ◽  
2021 ◽  
Author(s):  
Elias T. Lunsford ◽  
James C. Liao
Keyword(s):  
Lateral Line ◽  
Afferent Neurons ◽  
Posterior Lateral Line

scholarly journals Physiology of afferent neurons in larval zebrafish provides a functional framework for lateral line somatotopy

2012 ◽  
Vol 107 (10) ◽  
pp. 2615-2623 ◽  
Author(s):  
James C. Liao ◽  
Melanie Haehnel
Keyword(s):  
Firing Rate ◽  
Lateral Line ◽  
Input Resistance ◽  
Lateral Line System ◽  
Spontaneous Firing ◽  
Afferent Neurons ◽  
Line System ◽  
Larval Zebrafish ◽  
Posterior Lateral Line ◽  
Spontaneous Firing Rate

Fishes rely on the neuromasts of their lateral line system to detect water flow during behaviors such as predator avoidance and prey localization. Although the pattern of neuromast development has been a topic of detailed research, we still do not understand the functional consequences of its organization. Previous work has demonstrated somatotopy in the posterior lateral line, whereby afferent neurons that contact more caudal neuromasts project more dorsally in the hindbrain than those that contact more rostral neuromasts (Gompel N, Dambly-Chaudiere C, Ghysen A. Development 128: 387–393, 2001). We performed patch-clamp recordings of afferent neurons that contact neuromasts in the posterior lateral line of anesthetized, transgenic larval zebrafish ( Danio rerio) to show that larger cells are born earlier, have a lower input resistance, a lower spontaneous firing rate, and tend to contact multiple neuromasts located closer to the tail than smaller neurons, which are born later, have a higher input resistance, a higher spontaneous firing rate, and tend to contact single neuromasts. We suggest that early-born neurons are poised to detect large stimuli during the initial stages of development. Later-born neurons are more easily driven to fire and thus likely to be more sensitive to local, weaker flows. Afferent projections onto identified glutamatergic regions in the hindbrain lead us to hypothesize a novel mechanism for lateral line somatotopy. We show that afferent fibers associated with tail neuromasts respond to stronger stimuli and are wired to dorsal hindbrain regions associated with Mauthner-mediated escape responses and fast, avoidance swimming. The ability to process flow stimuli by circumventing higher-order brain centers would ease the task of processing where speed is of critical importance. Our work lays the groundwork to understand how the lateral line translates flow stimuli into appropriate behaviors at the single cell level.

Labeling Hair Cells and Afferent Neurons in the Posterior Lateral-Line System of Zebrafish

2013 ◽  
Vol 2013 (12) ◽  
pp. pdb.prot079467-pdb.prot079467 ◽  
Author(s):  
K. Schuster ◽  
A. Ghysen
Keyword(s):  
Lateral Line ◽  
Hair Cells ◽  
Lateral Line System ◽  
Afferent Neurons ◽  
Line System ◽  
Posterior Lateral Line

scholarly journals Organization and physiology of posterior lateral line afferent neurons in larval zebrafish

2010 ◽  
Vol 6 (3) ◽  
pp. 402-405 ◽  
Author(s):  
James C. Liao
Keyword(s):  
Lateral Line ◽  
Spatial Relationship ◽  
Genetic System ◽  
The Body ◽  
Lateral Line System ◽  
Afferent Neurons ◽  
Patch Clamp Recording ◽  
Line System ◽  
Larval Zebrafish ◽  
Posterior Lateral Line

The lateral line system of larval zebrafish can translate hydrodynamic signals from the environment to guide body movements. Here, I demonstrate a spatial relationship between the organization of afferent neurons in the lateral line ganglion and the innervation of neuromasts along the body. I developed a whole cell patch clamp recording technique to show that afferents innervate multiple direction-sensitive neuromasts, which are sensitive to low fluid velocities. This work lays the foundation to integrate sensory neuroscience and the hydrodynamics of locomotion in a model genetic system.

scholarly journals Frequency response properties of primary afferent neurons in the posterior lateral line system of larval zebrafish

2015 ◽  
Vol 113 (2) ◽  
pp. 657-668 ◽  
Author(s):  
Rafael Levi ◽  
Otar Akanyeti ◽  
Aleksander Ballo ◽  
James C. Liao
Keyword(s):  
Lateral Line ◽  
Sine Wave ◽  
Afferent Neuron ◽  
Lateral Line System ◽  
Afferent Neurons ◽  
Primary Afferent Neurons ◽  
Primary Afferent ◽  
Line System ◽  
Response Properties ◽  
Larval Zebrafish

The ability of fishes to detect water flow with the neuromasts of their lateral line system depends on the physiology of afferent neurons as well as the hydrodynamic environment. Using larval zebrafish ( Danio rerio), we measured the basic response properties of primary afferent neurons to mechanical deflections of individual superficial neuromasts. We used two types of stimulation protocols. First, we used sine wave stimulation to characterize the response properties of the afferent neurons. The average frequency-response curve was flat across stimulation frequencies between 0 and 100 Hz, matching the filtering properties of a displacement detector. Spike rate increased asymptotically with frequency, and phase locking was maximal between 10 and 60 Hz. Second, we used pulse train stimulation to analyze the maximum spike rate capabilities. We found that afferent neurons could generate up to 80 spikes/s and could follow a pulse train stimulation rate of up to 40 pulses/s in a reliable and precise manner. Both sine wave and pulse stimulation protocols indicate that an afferent neuron can maintain their evoked activity for longer durations at low stimulation frequencies than at high frequencies. We found one type of afferent neuron based on spontaneous activity patterns and discovered a correlation between the level of spontaneous and evoked activity. Overall, our results establish the baseline response properties of lateral line primary afferent neurons in larval zebrafish, which is a crucial step in understanding how vertebrate mechanoreceptive systems sense and subsequently process information from the environment.

scholarly journals Activity-independent specification of synaptic targets in the posterior lateral line of the larval zebrafish

2009 ◽  
Vol 106 (51) ◽  
pp. 21948-21953 ◽  
Author(s):  
A. Nagiel ◽  
S. H. Patel ◽  
D. Andor-Ardo ◽  
A. J. Hudspeth
Keyword(s):  
Lateral Line ◽  
Larval Zebrafish ◽  
Posterior Lateral Line

scholarly journals Morphology of lateral line canals in Neotropical freshwater stingrays (Chondrichthyes: Potamotrygonidae) from Negro River, Brazilian Amazon

2010 ◽  
Vol 8 (4) ◽  
pp. 867-876 ◽  
Author(s):  
Akemi Shibuya ◽  
Jansen Zuanon ◽  
Maria Lúcia G. de Araújo ◽  
Sho Tanaka
Keyword(s):  
Lateral Line ◽  
The Body ◽  
Mechanical Stimuli ◽  
High Concentration ◽  
Ventral Region ◽  
Negro River ◽  
Accurate Localization ◽  
Posterior Lateral Line ◽  
The Family ◽  
The Relationship

The relationship between the distribution of the lateral line canals and their functionality has not been well examined in elasmobranchs, especially among Neotropical freshwater stingrays of the family Potamotrygonidae. The spatial distribution of the canals and their tubules and the quantification of the neuromasts were analyzed in preserved specimens of Potamotrygon motoro, P. orbignyi, Potamotrygon sp. "cururu", and Paratrygon aiereba from the middle Negro River, Amazonas, Brazil. The hyomandibular, infraorbital, posterior lateral line, mandibular, nasal and supraorbital canals were characterized and their pores and neuromasts quantified. The ventral canals are known to facilitate the accurate localization of prey items under the body, and our results indicate that the dorsal canals may be employed in identifying the presence of predators or potential prey positioned above the stingray's body. The presence of non-pored canals in the ventral region may be compensated by the high concentration of neuromasts found in the same area, which possibly allow the accurate detection of mechanical stimuli. The concentration of non-pored canals near the mouth indicates their importance in locating and capturing prey buried in the bottom substrate, possibly aided by the presence of vesicles of Savi.

scholarly journals Development of the posterior lateral line system in Thunnus thynnus, the atlantic blue-fin tuna, and in its close relative Sarda sarda

2010 ◽  
Vol 54 (8-9) ◽  
pp. 1317-1322 ◽  
Author(s):  
Alain Ghysen ◽  
Kevin Schuster ◽  
Denis Coves ◽  
Fernando de la Gandara ◽  
Nikos Papandroulakis ◽  
...  
Keyword(s):  
Lateral Line ◽  
Close Relative ◽  
Lateral Line System ◽  
Thunnus Thynnus ◽  
Line System ◽  
Posterior Lateral Line ◽  
Sarda Sarda

scholarly journals Notch-restricted Atoh1 expression regulates morphogenesis of the posterior lateral line in zebrafish

2010 ◽  
Vol 344 (1) ◽  
pp. 449
Author(s):  
Miho Matsuda ◽  
Ajay Chitnis
Keyword(s):  
Lateral Line ◽  
Posterior Lateral Line
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