Rodent Estrous Cycle Monitoring utilizing Vaginal Lavage: No Such Thing As a Normal Cycle

10.3791/62884 ◽  
2021 ◽  
Author(s):  
Hannah Robert ◽  
Lindsay Ferguson ◽  
Olivia Reins ◽  
Tiffany Greco ◽  
Mayumi L. Prins ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Normal Cycle ◽  
Vaginal Lavage ◽  
Cycle Monitoring

scholarly journals Estrous Cycle Monitoring in Mice with Rapid Data Visualization and Analysis

BIO-PROTOCOL ◽  
2019 ◽  
Vol 9 (17) ◽  
Author(s):  
Leanna Pantier ◽  
Jiang Li ◽  
Catherine Christian
Keyword(s):  
Estrous Cycle ◽  
Data Visualization ◽  
Cycle Monitoring

scholarly journals Comparative Vaginal Cytology of the Estrous Cycle of Black-Footed Ferrets (Mustela Nigripes), Siberian Polecats (M. Eversmanni), and Domestic Ferrets (M. Putorius Furo)

1992 ◽  
Vol 4 (1) ◽  
pp. 38-44 ◽  
Author(s):  
Elizabeth S. Williams ◽  
E. Tom Thorne ◽  
Donald R. Kwiatkowski ◽  
Kim Lutz ◽  
Sandy L. Anderson
Keyword(s):  
Epithelial Cells ◽  
Estrous Cycle ◽  
Reproductive Cycle ◽  
The Other ◽  
Critically Endangered ◽  
Vaginal Lavage ◽  
Mustela Nigripes ◽  
Reproductive Management ◽  
Vaginal Cytology ◽  
Endangered Animals

Vaginal cytology and vulva size were used to characterize the reproductive cycle of female black-footed ferrets ( Mustela nigripes), Siberian polecats ( M. eversmanni), and domestic ferrets ( M. putorius furo). Emphasis was on black-footed ferrets because of the need to breed these critically endangered animals and on Siberian polecats because of the close taxonomic relationship to black-footed ferrets. Vaginal cytology of the 3 species of ferret is similar. Proestrus was characterized by an increasing percentage of superficial epithelial cells and enlargement of the vulva. During estrus, superficial cells were usually ≥ 90% of epithelial cells in the vaginal lavage and after several days were fully keratinized. Neutrophils were more common during all stages of the estrous cycle in domestic ferrets than they were in the other species. Following copulation, percentage of superficial calls in the vagina declined and vulva swelling subsided. Large cells, probably of uterine symplasma origin, were observed in vaginal lavages following whelping or pseudopregnancy. Vaginal cytology is extremely useful in the reproductive management of black-footed ferrets and Siberian polecats. Knowledge of normal vaginal cytology could be applied to the diagnosis of female reproductive abnormalities in all 3 species.

Rat Ovarian Renin: Characterization and Changes during the Estrous Cycle*

Endocrinology ◽  
1988 ◽  
Vol 123 (5) ◽  
pp. 2331-2340 ◽  
Author(s):  
RANDY B. HOWARD ◽  
ANTHONY G. PUCELL ◽  
F. MERLIN BUMPUS ◽  
AHSAN HUSAIN
Keyword(s):  
Estrous Cycle

scholarly journals Full life-cycle monitoring and earlier warning for bolt joint loosening using modified vibro-acoustic modulation

2022 ◽  
Vol 162 ◽  
pp. 108054
Author(s):  
Xiaoshu Qin ◽  
Chang Peng ◽  
Gaozheng Zhao ◽  
Zengye Ju ◽  
Shanshan Lv ◽  
...  
Keyword(s):  
Life Cycle ◽  
Full Life Cycle ◽  
Full Life ◽  
Acoustic Modulation ◽  
Cycle Monitoring

scholarly journals 140 Effect of hCG on the last day of the 5-d CIDR-Synch and/or on day 5 of the estrous cycle on luteal dynamics in recipient Holstein heifers

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 112-113
Author(s):  
Marcelo Siqueira El Azzi ◽  
Everardo Anta Galvan ◽  
Teresita Valdes Arciniega ◽  
Iago Leão ◽  
Rodrigo Sala ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Ovulation Rate

Abstract The objective was to determine the effect of hCG (3,300 IU) administered on days 0 and/or 5 of the estrous cycle on total luteal area (TLA) and circulating concentrations of progesterone (P4) in recipient heifers. All heifers (n = 232; BCS = 3.3 ± 0.2) were synchronized with a 5d CIDR-Synch protocol (d-8: used CIDR inserted; d-3: CIDR removed and PGF2α treatment; d0: 100µg GnRH or hCG). Heifers were randomly assigned to four treatments: control, hCGd0, hCGd5, and hCGd0&5. Controls were treated with GnRH on d0, while hCGd0 received hCG on d0. hCGd5 heifers were treated with GnRH on d0 and hCG on d5, while hCGd0&5 received hCG on d0 and 5. Ovaries were scanned by ultrasound on d0, 5, and 12. Blood was collected on d0, 5, 7, and 12. Synchronization rate (94%) did not differ (P = 0.94) by treatment. Treatment differences were only tested in synchronized heifers (n = 218). Proportion of heifers with 1 or 2+ CL on d5 did not differ (P = 0.10) for hCG d0 treatments (hCGd0 + hCGd0&5) vs. GnRH d0 treatments (controls + hCGd5). However, heifers treated with hCG on d0 had greater (P < 0.01) TLA and P4 on d5 vs. treatments with GnRH on d0 (311 ± 13 vs. 257 ± 9 mm2; and 2.39 ± 0.15 vs. 1.90 ± 0.09 ng/mL). Ovulation rate for d5 hCG did not differ (P = 0.63) for hCGd5 vs. hCGd0&5 (93 vs. 84%). Controls had the lowest serum P4 on d7 and 12. In contrast, hCGd0&5 had the highest serum P4 on d7 (Table 1). Serum P4 on d7 did not differ for hCGd0 vs. hCGd5. On d12, serum P4 and TLA were not different for hCGd5 vs. hCGd0&5. These data indicate that hCG can be used on d0 to induce an increase in serum P4 on d5 compared to GnRH. However, only heifers treated with hCG on d5 achieved mean serum P4 > 8ng/mL.

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