A Study of The Histopathological Effect of White Albino Mice Lung Infected with Aspergillus Fumigatus, Treated with Amphotericin B, And  Trametes Spp.

The present study was designed to investigate the histological lesions in the lungs of white mice infected with Aspergillus fumigatus by intraperitoneal injection, nasal implantation, the current research also examined an attempt to treat these tissue lesions by using both of the antifungal Amphotericin B and the alcoholic extract of Trametes for two weeks. The results showed that A. fumigatus led to tissue changes represented by the degeneration, desquamation the lining respiratory epithelium of the bronchiole, thickening, rupturing of the alveolar walls, congestion of the vessels and lymphocytic infiltration, the lungs of mice that infected with A. fumigatus and treated with the antifungal Amphotericin B and alcoholic extract of the Trametes spp. showed improvement, as degeneration and desquamation of the lining of the respiratory epithelium of the trachea, the presence of dust cells, lymphocytic infiltration, in addition to the appearance of semi-normal lung tissue in another histological also were observed. We conclude that Amphotericin B and alcoholic extract of Trametes spp. have activity in treating the histological lesions that resulted from A. fumigatus infection.

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Metformin Protects against Radiation-Induced Acute Effects by Limiting Senescence of Bronchial-Epithelial Cells

International Journal of Molecular Sciences ◽

10.3390/ijms22137064 ◽

2021 ◽

Vol 22 (13) ◽

pp. 7064

Author(s):

Christine Hansel ◽

Samantha Barr ◽

Alina V. Schemann ◽

Kirsten Lauber ◽

Julia Hess ◽

...

Keyword(s):

Epithelial Cells ◽

Lung Tissue ◽

Cellular Stress ◽

Lung Epithelial Cells ◽

Normal Lung Tissue ◽

Normal Lung ◽

Metformin Treatment ◽

Acute Effects ◽

Lung Epithelial ◽

Radiation Induced

Radiation-induced damage to normal lung parenchyma remains a dose-limiting factor in thorax-associated radiotherapy (RT). Severe early and late complications with lungs can increase the risk of morbidity in cancer patients after RT. Herein, senescence of lung epithelial cells following RT-induced cellular stress, or more precisely the respective altered secretory profile, the senescence-associated secretory phenotype (SASP), was suggested as a central process for the initiation and progression of pneumonitis and pulmonary fibrosis. We previously reported that abrogation of certain aspects of the secretome of senescent lung cells, in particular, signaling inhibition of the SASP-factor Ccl2/Mcp1 mediated radioprotection especially by limiting endothelial dysfunction. Here, we investigated the therapeutic potential of a combined metformin treatment to protect normal lung tissue from RT-induced senescence and associated lung injury using a preclinical mouse model of radiation-induced pneumopathy. Metformin treatment efficiently limited RT-induced senescence and SASP expression levels, thereby limiting vascular dysfunctions, namely increased vascular permeability associated with increased extravasation of circulating immune and tumor cells early after irradiation (acute effects). Complementary in vitro studies using normal lung epithelial cell lines confirmed the senescence-limiting effect of metformin following RT finally resulting in radioprotection, while fostering RT-induced cellular stress of cultured malignant epithelial cells accounting for radiosensitization. The radioprotective action of metformin for normal lung tissue without simultaneous protection or preferable radiosensitization of tumor tissue might increase tumor control probabilities and survival because higher radiation doses could be used.

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