Potential role for Trichoderma harzianum, Clonostachys rosea and salicylic acid to induce resistance of date palm roots to Bayoud disease (Fusarium oxysporum f. sp. albedinis)

Background: In the present study, a bioguided fractionation was realized to isolate bioactive compounds on Fusarium oxysporum f.sp. albedinis (Foa) from Citrullus colocynthis L. Foa is the causal agent of “ Bayoud”, a lethal disease of date palm. Methods: Extracts of Citrullus colocynthis fruits peels using four solvents (n-hexane, dichloromethane, ethanol, chloroform) were tested for antifungal effect on Foa with radial growth technique. The ethanolic extract was selected for fractionation to isolate bioactive compounds (Percentage of Growth Reduction “PGR”: 63.6%). The fractionation was realized using six solvents (hexane, dichloromethane, ethyl acetate, methanol, butanol, water). Through fractionation, methanolic and butanolic fractions (PGR= 57.6% and 69.7%, respectively) had been chosen for bioactive compounds isolation. Results: Chromatographic and spectroscopic analyses had led to isolation of six compounds deduced as: (C1): 2-O-β-D-glucopyranosylcucurbitacin E; (C2): 2-(Nonan-8-one)-(1H)-4-quinolone; (C3): 2- (Nonan-8-one) 4-methoxy-quinoline, (C4): Isosaponarin; (C5): Isovitexin; (C6): Isoorientin 3’-Omethyl ether. The isolated compounds had been tested for antifungal effect. The compound (C1) represents the best effect among isolated compounds (PGR, up to 54.5%). Conclusion: The efficiency of (C1) reflects its opportunity to be used for the development of efficient treatment against Bayoud disease.

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Exploring the potential of using bioactive plant products in the management of Fusarium oxysporum f.sp. albedinis: the causal agent of Bayoud disease on date palm (Phoenix dactylifera L.)

Beni-Suef University Journal of Basic and Applied Sciences ◽

10.1186/s43088-020-00071-x ◽

2020 ◽

Vol 9 (1) ◽

Author(s):

Eimad Dine Tariq Bouhlali ◽

Mgal Derouich ◽

Houria Ben-Amar ◽

Reda Meziani ◽

Adil Essarioui

Keyword(s):

Antifungal Activity ◽

Fusarium Oxysporum ◽

Plant Extracts ◽

Date Palm ◽

Antioxidant Properties ◽

Phoenix Dactylifera ◽

Trolox Equivalent Antioxidant Capacity ◽

Dependent Manner ◽

Antioxidant Power ◽

Bayoud Disease

Abstract Background “Bayoud” disease caused by Fusarium oxysporum f. sp. albedinis (Foa) poses a serious threat to date palm (Phoenix dactylifera L.) in Morocco. However, research studies performed to discover biological methods to control this disease remain limited. The present study has set objectives to determine antifungal activity of five plants extracts (Acacia cyanophylla, Cupressus atlantica, Eucalyptus torquata, Nerium oleander, and Schinus molle) against Foa and link this effect to their content in polyphenols and flavonoids as well as their antioxidant properties. Results Plant extracts showed significant differences (p < 0.05) regarding their antifungal activity. The extracts of E. torquata and C. atlantica showed the strongest antifungal effect resulting in the inhibition of mycelial growth, sporulation, and spore germination in a dose-dependent manner. In addition, there were significant differences among the examined plant extracts in respect to their total polyphenols (1.536–7.348 g GAE/100 g DW), flavonoids (0.986–5.759 g RE/100 g DW), and antioxidant properties measured by Trolox equivalent antioxidant capacity (TEAC) (7.47–38.97 mmol TE/100 g DW) and ferric-reducing antioxidant power (FRAP) assay (8.95–47.36 mmol TE/100 g DW). Moreover, the antifungal potential of plant extracts was found to be moderately to strongly correlated with their polyphenol and flavonoid contents as well as their antioxidant activity, implying that the effective inhibitory activity of these plant extracts is partly due to their richness in antioxidative secondary metabolites. Conclusion Our findings shed further light on plants as a-yet-untapped resource of bioactive compounds and constructed the foundation for the development of new biological approaches to best manage Bayoud disease.

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