A Cluster of Nucleotide‐Binding Site–Leucine‐Rich Repeat Genes Resides in a Barley Powdery Mildew Resistance Quantitative Trait Loci on 7HL

Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75–0.87, which represents 12% segment of the long arm of chromosome 2Ss#1. In this study, we performed RNA-seq using RNA extracted from leaf samples of three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines at 0, 12, 24, and 48 h after inoculation with Bgt isolates. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homologous (Ph) genes. After analyzing the 46 ph1b-induced 2Ss#1L recombinants in the region where Pm57 is located with different Bgt-responses, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese Spring (T. aestivum L.) with other model species, we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens.

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Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii

10.20944/preprints201912.0322.v1 ◽

2019 ◽

Author(s):

Zhenjie Dong ◽

Xiubin Tian ◽

Chao Ma ◽

Qing Xia ◽

Beilin Wang ◽

...

Keyword(s):

Molecular Markers ◽

Powdery Mildew ◽

Binding Site ◽

Resistance Gene ◽

Resistance Genes ◽

Powdery Mildew Resistance ◽

Nucleotide Binding ◽

Leucine Rich Repeat ◽

Powdery Mildew Resistance Gene ◽

Mildew Resistance

Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75-0.87, which represents 12% of 2Ss#1 segment on the long arm of chromosome 2Ss#1. In this study, we performed RNA-Seq on three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines with Bgt-isolates inoculation at 0, 12, 24, and 48 hours after inoculation. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homoelogous (Ph) genes. Analysis of the 46 ph1b-induced 2Ss#1L recombinants with different Bgt-responses using 28 2Ss#1L-specific molecular markers in the interval FL0.72-0.87 where Pm57 is located, and the flanking intervals, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese spring (T. aestivum L.) with other model species we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for further cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens.

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Major Quantitative Trait Loci and Putative Candidate Genes for Powdery Mildew Resistance and Fruit-Related Traits Revealed by an Intraspecific Genetic Map for Watermelon (Citrullus lanatus var. lanatus)

PLoS ONE ◽

10.1371/journal.pone.0145665 ◽

2015 ◽

Vol 10 (12) ◽

pp. e0145665 ◽

Cited By ~ 16

Author(s):

Kwang-Hwan Kim ◽

Ji-Hyun Hwang ◽

Dong-Yeup Han ◽

Minkyu Park ◽

Seungill Kim ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Powdery Mildew ◽

Candidate Genes ◽

Genetic Map ◽

Quantitative Trait ◽

Powdery Mildew Resistance ◽

Citrullus Lanatus ◽

Mildew Resistance ◽

Trait Loci ◽

Intraspecific Genetic Map

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Quantitative Trait Loci Mapping for Adult-Plant Resistance to Powdery Mildew in Bread Wheat

Phytopathology ◽

10.1094/phyto-96-0784 ◽

2006 ◽

Vol 96 (7) ◽

pp. 784-789 ◽

Cited By ~ 44

Author(s):

S. S. Liang ◽

K. Suenaga ◽

Z. H. He ◽

Z. L. Wang ◽

H. Y. Liu ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Powdery Mildew ◽

Plant Resistance ◽

Quantitative Trait ◽

Powdery Mildew Resistance ◽

Adult Plant Resistance ◽

Adult Plant ◽

Phenotypic Variance ◽

Mildew Resistance ◽

Trait Loci

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a major disease to wheat (Triticum aestivum) worldwide. Use of adult-plant resistance (APR) is an effective method to develop wheat cultivars with durable resistance to powdery mildew. In the present study, 432 molecular markers were used to map quantitative trait loci (QTL) for APR to powdery mildew in a doubled haploid (DH) population with 107 lines derived from the cross Fukuho-komugi × Oligoculm. Field trials were conducted in Beijing and Anyang, China during 2003-2004 and 2004-2005 cropping seasons, respectively. The DH lines were planted in a randomized complete block design with three replicates. Artificial inoculation was carried out in Beijing with highly virulent isolate E20 of B. graminis f. sp. tritici and the powdery mildew severity on penultimate leaf was evaluated four times, and the maximum disease severity (MDS) on penultimate leaf was investigated in Anyang under natural inoculation in May 2004 and 2005. The heritability of resistance to powdery mildew for MDS in 2 years and two locations ranged from 0.82 to 0.93, while the heritability for area under the disease progress curve was between 0.84 and 0.91. With the method of composite interval mapping, four QTL for APR to powdery mildew were detected on chromosomes 1AS, 2BL, 4BL, and 7DS, explaining 5.7 to 26.6% of the phenotypic variance. Three QTL on chromosomes 1AS, 2BL, and 7DS were derived from the female, Fukuho-komugi, while the one on chromosome 4BL was from the male, Oligoculm. The QTL on chromosome 1AS showed high genetic effect on powdery mildew resistance, accounting for 19.5 to 26.6% of phenotypic variance across two environments. The QTL on 7DS associated with the locus Lr34/Yr18, flanked by microsatellite Xgwm295.1 and Ltn (leaf tip necrosis). These results will benefit for improving powdery mildew resistance in wheat breeding programs.

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Identification of quantitative trait loci associated with powdery mildew resistance in mungbean using ISSR and ISSR-RGA markers

Molecular Breeding ◽

10.1007/s11032-017-0753-2 ◽

2017 ◽

Vol 37 (12) ◽

Cited By ~ 3

Author(s):

Oythip Poolsawat ◽

Chitpan Kativat ◽

Kitiya Arsakit ◽

Piyada Alisha Tantasawat

Keyword(s):

Quantitative Trait Loci ◽

Powdery Mildew ◽

Quantitative Trait ◽

Powdery Mildew Resistance ◽

Mildew Resistance ◽

Trait Loci

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The wheat homolog of putative nucleotide-binding site–leucine-rich repeat resistance gene TaRGA contributes to resistance against powdery mildew

Functional & Integrative Genomics ◽

10.1007/s10142-015-0471-y ◽

2016 ◽

Vol 16 (2) ◽

pp. 115-126 ◽

Cited By ~ 6

Author(s):

Defu Wang ◽

Xiaobing Wang ◽

Yu Mei ◽

Hansong Dong

Keyword(s):

Powdery Mildew ◽

Binding Site ◽

Resistance Gene ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Leucine Rich Repeat

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Basal Host Resistance of Barley to Powdery Mildew: Connecting Quantitative Trait Loci and Candidate Genes

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-23-1-0091 ◽

2010 ◽

Vol 23 (1) ◽

pp. 91-102 ◽

Cited By ~ 71

Author(s):

Reza Aghnoum ◽

Thierry C. Marcel ◽

Annika Johrde ◽

Nicola Pecchioni ◽

Patrick Schweizer ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Powdery Mildew ◽

Candidate Genes ◽

Quantitative Trait ◽

Powdery Mildew Resistance ◽

Adult Plant ◽

Basal Resistance ◽

Mildew Resistance ◽

Trait Loci ◽

Mapping Populations

The basal resistance of barley to powdery mildew (Blumeria graminis f. sp. hordei) is a quantitatively inherited trait that is based on nonhypersensitive mechanisms of defense. A functional genomic approach indicates that many plant candidate genes are involved in the defense against formation of fungal haustoria. It is not known which of these candidate genes have allelic variation that contributes to the natural variation in powdery mildew resistance, because many of them may be highly conserved within the barley species and may act downstream of the basal resistance reaction. Twenty-two expressed sequence tag or cDNA clone sequences that are likely to play a role in the barley–Blumeria interaction based on transcriptional profiling, gene silencing, or overexpression data, as well as mlo, Ror1, and Ror2, were mapped and considered candidate genes for contribution to basal resistance. We mapped the quantitative trait loci (QTL) for powdery mildew resistance in six mapping populations of barley at seedling and adult plant stages and developed an improved high-density integrated genetic map containing 6,990 markers for comparing QTL and candidate gene positions over mapping populations. We mapped 12 QTL at seedling stage and 13 QTL at adult plant stage, of which four were in common between the two developmental stages. Six of the candidate genes showed coincidence in their map positions with the QTL identified for basal resistance to powdery mildew. This co-localization justifies giving priority to those six candidate genes to validate them as being responsible for the phenotypic effects of the QTL for basal resistance.

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Mapping Quantitative Trait Loci for Powdery Mildew Resistance in Flax (Linum usitatissimum L.)

Crop Science ◽

10.2135/cropsci2013.05.0298 ◽

2013 ◽

Vol 53 (6) ◽

pp. 2462-2472 ◽

Cited By ~ 14

Author(s):

Parvaneh Asgarinia ◽

Sylvie Cloutier ◽

Scott Duguid ◽

Khalid Rashid ◽

AghaFakhr Mirlohi ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Powdery Mildew ◽

Quantitative Trait ◽

Linum Usitatissimum ◽

Powdery Mildew Resistance ◽

Mildew Resistance ◽

Linum Usitatissimum L ◽

Trait Loci

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Genetic Analysis of Victorin Sensitivity and Identification of a Causal Nucleotide-Binding Site Leucine-Rich Repeat Gene in Phaseolus vulgaris

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-12-17-0328-r ◽

2018 ◽

Vol 31 (10) ◽

pp. 1069-1074 ◽

Cited By ~ 2

Author(s):

J. M. Lorang ◽

C. H. Hagerty ◽

R. Lee ◽

P. E. McClean ◽

T. J. Wolpert

Keyword(s):

Cell Death ◽

Phaseolus Vulgaris ◽

Binding Site ◽

Quantitative Trait ◽

Phenotypic Variability ◽

Recombinant Inbred Lines ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Leucine Rich Repeat ◽

Dependent Cell

Cochliobolus victoria, the causal agent of Victoria blight, is pathogenic due to its production of a toxin called victorin. Victorin sensitivity in oats, barley, Brachypodium spp., and Arabidopsis has been associated with nucleotide-binding site leucine-rich repeat (NLR) genes, a class of genes known for conferring disease resistance. In this work, we investigated the sensitivity of Phaseolus vulgaris to victorin. We found that victorin sensivity in Phaseolus vulgaris is a developmentally regulated, quantitative trait. A single quantitative trait locus (QTL) accounted for 34% of the phenotypic variability in victorin sensitivity among Stampede × Red Hawk (S×R) recombinant inbred lines. We cloned two NLR-encoding genes within this QTL and showed one, Phvul05G031200 (PvLOV), confers victorin-dependent cell death when overexpressed in Nicotiana benthamiana. Protein sequences of PvLOV from victorin-sensitive and the victorin-resistant bean parents differ by two amino acids in the leucine-rich repeat region, but both proteins confer victorin-dependent cell death when overexpressed in N. benthamiana.

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