Characterization of the Glycogen Branching Enzyme from Pectobacterium carotovorum subsp. carotovorum LY34

Corynebacterium glutamicum transiently accumulates glycogen as carbon capacitor during the early exponential growth phase in media containing carbohydrates. In some bacteria glycogen is synthesized by the consecutive action of ADP-glucose pyrophosphorylase (GlgC), glycogen synthase (GlgA) and glycogen branching enzyme (GlgB). GlgC and GlgA of C. glutamicum have been shown to be necessary for glycogen accumulation in this organism. However, although cg1381 has been annotated as the putative C. glutamicum glgB gene, cg1381 and its gene product have not been characterized and their role in transient glycogen accumulation has not yet been investigated. We show here that the cg1381 gene product of C. glutamicum catalyses the formation of α-1,6-glycosidic bonds in polysaccharides and thus represents a glycogen branching enzyme. RT-PCR experiments revealed glgB to be co-transcribed with glgE, probably encoding a maltosyltransferase. Promoter activity assays with the glgE promoter region revealed carbon-source-dependent expression of the glgEB operon. Characterization of the growth and glycogen content of glgB-deficient and glgB-overexpressing strains showed that the glycogen branching enzyme GlgB is essential for glycogen formation in C. glutamicum. Taken together these results suggest that an interplay of the enzymes GlgC, GlgA and GlgB is not essential for growth, but is required for synthesis of the transient carbon capacitor glycogen in C. glutamicum.

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Characterization of substrate and product specificity of the purified recombinant glycogen branching enzyme of Rhodothermus obamensis

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/j.bbagen.2012.09.022 ◽

2013 ◽

Vol 1830 (1) ◽

pp. 2167-2177 ◽

Cited By ~ 37

Author(s):

Xavier Roussel ◽

Christine Lancelon-Pin ◽

Anders Viksø-Nielsen ◽

Agnès Rolland-Sabaté ◽

Florent Grimaud ◽

...

Keyword(s):

Branching Enzyme ◽

Product Specificity ◽

Glycogen Branching Enzyme

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Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage

Microbiology ◽

10.1099/mic.0.067280-0 ◽

2013 ◽

Vol 159 (Pt_7) ◽

pp. 1487-1496 ◽

Cited By ~ 38

Author(s):

Dong Hwan Lee ◽

Jeong-A Lim ◽

Juneok Lee ◽

Eunjung Roh ◽

Kyusuk Jung ◽

...

Keyword(s):

Chinese Cabbage ◽

Pectobacterium Carotovorum ◽

Carotovorum Subsp

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Characterization of Pectobacterium carotovorum subsp. carotovorum causing soft-rot disease on Pinellia ternata in China

European Journal of Plant Pathology ◽

10.1007/s10658-007-9219-4 ◽

2007 ◽

Vol 120 (3) ◽

pp. 305-310 ◽

Cited By ~ 14

Author(s):

Xiu-Fang Hu ◽

Fei-Xiang Ying ◽

Yu-Bo He ◽

Yuan-Yuan Gao ◽

Hai-Min Chen ◽

...

Keyword(s):

Soft Rot ◽

Pectobacterium Carotovorum ◽

Pinellia Ternata ◽

Soft Rot Disease ◽

Carotovorum Subsp ◽

Rot Disease

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Characterization of the branching patterns of glycogen branching enzyme truncated on the N-terminus

Archives of Biochemistry and Biophysics ◽

10.1016/s0003-9861(03)00341-2 ◽

2003 ◽

Vol 418 (1) ◽

pp. 34-38 ◽

Cited By ~ 38

Author(s):

Claire H Devillers ◽

Mary E Piper ◽

Miguel A Ballicora ◽

Jack Preiss

Keyword(s):

Branching Enzyme ◽

Branching Patterns ◽

N Terminus ◽

Glycogen Branching Enzyme

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Identification and characterization of the bacteriocin Carocin S3 from the multiple bacteriocin producing strain of Pectobacterium carotovorum subsp. carotovorum

BMC Microbiology ◽

10.1186/s12866-020-01955-9 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Jyun-Wei Wang ◽

Reymund C. Derilo ◽

Ruchi Briam James S. Lagitnay ◽

Huang-Pin Wu ◽

Kai-In Chen ◽

...

Keyword(s):

Pectobacterium Carotovorum ◽

Carotovorum Subsp ◽

Identification And Characterization

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Erratum to “Characterization of the branching patterns of glycogen branching enzyme truncated on the N-terminus” [Arch. Biochem. Biophys. 418 (2003) 34–38]

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2003.10.015 ◽

2004 ◽

Vol 421 (2) ◽

pp. 290

Author(s):

Claire H Devillers ◽

Mary E Piper ◽

Miguel A Ballicora ◽

Jack Preiss

Keyword(s):

Branching Enzyme ◽

Branching Patterns ◽

N Terminus ◽

Glycogen Branching Enzyme

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Characterization of onion soft rot bacteria in Bangladesh

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v52i3.34157 ◽

2017 ◽

Vol 52 (3) ◽

pp. 209-220

Author(s):

MM Rahman ◽

MAA Khan ◽

IH Mian ◽

AM Akanda ◽

MZ Alam

Keyword(s):

Bacterial Pathogens ◽

Soft Rot ◽

Pectobacterium Carotovorum ◽

Bacterial Isolates ◽

Biochemical Tests ◽

Carotovorum Subsp ◽

O 15 ◽

O 69 ◽

Physiological And Biochemical

A study was undertaken for characterization and identification of the soft rot causing bacterial pathogens of onion. Bacterial pathogens were isolated from soft rotted stored onions of different varieties and locations of Bangladesh. Altogether 73 bacterial isolates were isolated from soft rotted onions. Among them, twelve soft rot-positive isolates were selected for characterization and identification on the basis of more virulence. Physiological and biochemical tests were performed following standard methods for characterization and identification of selected soft rot bacterial isolates. Seven isolates namely O-03, O-18, O-69, O-72, O-130, O-156 and O-180 were identified as Pectobacterium carotovorum subsp. carotovorum (E. carotovora subsp. carotovora), two isolates O-101 and O-118 were identified as E. chrysanthemi and three isolates O-05, O-14 and O-15 were the members of Burkholderia cepacia.Bangladesh J. Sci. Ind. Res. 52(3), 209-220, 2017

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