scholarly journals AUTOMATIC SELECTION OF TWO-DIMENSIONAL ELECTROPHORESIS GEL WITH LEAST GEOMETRIC DISTORTIONS

2010 ◽  
Vol 2 (1) ◽  
pp. 9-13
Author(s):  
Dalius Matuzevičius
Keyword(s):  
Quality Criterion ◽  
Horizontal Line ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Two Dimensional Electrophoresis ◽  
Geometric Distortions ◽  
Biochemical Experiment ◽  
Dimensional Electrophoresis ◽  
Selection Of

The paper presents an algorithm for automated selection of the highest quality two-dimensional gel electrophoresis image. The quality criterion is the amount of vertical geometric distortions of the gel. The aim is to select the least distorted gel from the group received during the same biochemical experiment. Vertical geometric distortions displace proteins of the same molecular mass from the horizontal line and have a greater impact on the determination of protein characteristics than horizontal distortions. After presenting algorithm for evaluation of distortions and selection of base gel results are compared to expert's made selections. If necessary, algorithm may be adapted for horizontal distortion evaluation.

scholarly journals Identification of protein disulphide-isomerase as a major acidic polypeptide in rat liver microsomal membranes

1983 ◽  
Vol 213 (1) ◽  
pp. 245-248 ◽  
Author(s):  
E N C Mills ◽  
N Lambert ◽  
R B Freedman
Keyword(s):  
Liver Enzyme ◽  
Rat Liver ◽  
Peptide Mapping ◽  
Bovine Liver ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Protein Disulphide Isomerase ◽  
Two Dimensional Electrophoresis ◽  
Microsomal Membranes ◽  
Dimensional Electrophoresis

Protein disulphide-isomerase was purified to homogeneity from rat liver by a rapid high-yielding procedure. Structural properties of the pure enzyme were very similar to those of the bovine liver enzyme purified by the same method. The purified rat liver enzyme was subjected to two-dimensional gel electrophoresis in the presence and in the absence of microsomal membranes, and shown to co-electrophorese with a major acidic polypeptide clearly identifiable in the two-dimensional electrophoretic profile of microsomal membranes. This identification was confirmed by peptide ‘mapping’ of the pure enzyme and of the defined spot from a two-dimensional electrophoresis gel.

Two-Dimensional Electrophoresis-Based Selection of Aptamers Against an Unidentified Protein in a Tissue Sample

2013 ◽  
Vol 46 (18) ◽  
pp. 2954-2963 ◽  
Author(s):  
Nasa Savory ◽  
Shinichi Goto ◽  
Wataru Yoshida ◽  
Yutaka Unuma ◽  
Makoto Nakamura ◽  
...  
Keyword(s):  
Tissue Sample ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Selection Of

scholarly journals LACK OF GENIC VARIATION IN THE ABUNDANT PROTEINS OF HUMAN KIDNEY

Genetics ◽  
1980 ◽  
Vol 96 (4) ◽  
pp. 967-974
Author(s):  
Sandra C Smith ◽  
Robert R Racine ◽  
Charles H Langley
Keyword(s):  
Human Kidney ◽  
Two Dimensional ◽  
Electrophoretic Variation ◽  
Two Dimensional Electrophoresis ◽  
Genic Variation ◽  
Dimensional Electrophoresis ◽  
Selection Of

ABSTRACT Abundant proteins of 25 human kidneys were surveyed for genic variation by means of two-dimensional electrophoresis. Eighty-three (83) proteins were scored, and no genic variation was detected. This reduction in genic heterozygosity corroborates results determined with two-dimensional electrophoresis in mice and flies. These results suggest that previous estimates of electrophoretic variation may have been in error because of biased selection of loci.

Proteins of human urine. II. Identification by two-dimensional electrophoresis of a new candidate marker for prostatic cancer.

1982 ◽  
Vol 28 (1) ◽  
pp. 160-163 ◽  
Author(s):  
J J Edwards ◽  
N G Anderson ◽  
S L Tollaksen ◽  
A C von Eschenbach ◽  
J Guevara
Keyword(s):  
Sodium Dodecyl ◽  
Prostatic Hyperplasia ◽  
Prostatic Adenocarcinoma ◽  
Relative Molecular Mass ◽  
Urogenital System ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Candidate Marker ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Abstract A protein series common to the urine and prostatic tissue of 16 of 17 patients with prostatic adenocarcinoma has been identified by high-resolution two-dimensional gel electrophoresis. These proteins, designated PCA-1, have a relative molecular mass in sodium dodecyl sulfate of about 40000. Analyses of urines from eight age-matched controls, seven patients with other ty pes of urogenital malignancies, two patients with benign prostatic hyperplasia, and five patients with malignancies not associated with the urogenital system failed to show PCA-1 in the patterns. These preliminary findings suggest that this protein should be systematically investigated as a candidate marker for prostatic adenocarcinoma in man.

Muscle protein analysis. III. Analysis of solubilized frozen-tissue sections by two-dimensional electrophoresis.

1981 ◽  
Vol 27 (11) ◽  
pp. 1918-1921 ◽  
Author(s):  
C S Giometti ◽  
N G Anderson
Keyword(s):  
Gel Electrophoresis ◽  
Muscle Protein ◽  
Electrophoretic Analysis ◽  
Human Muscle ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Two Dimensional Electrophoresis ◽  
Tissue Sections ◽  
Frozen Tissue ◽  
Dimensional Electrophoresis

Abstract Proteins from frozen histological sections of human muscle were analyzed by two-dimensional gel electrophoresis. Patterns so obtained were identical to those from whole homogenates of muscle prepared from frozen tissue powders that had much higher protein concentrations. To increase the number of proteins visible on gels of samples low in protein content, the gels were silver stained, or the proteins were labeled with [14C]iodoacetamide before electrophoresis and the gels were fluorographed. The latter method allow use of a single frozen-tissue section for two-dimensional electrophoretic analysis and brings the technique closer to practicable clinical use.

Quantitative determination of haptoglobin glycoform variants in psoriasis

2010 ◽  
Vol 391 (12) ◽  
Author(s):  
Bernardetta Maresca ◽  
Luisa Cigliano ◽  
Maria M. Corsaro ◽  
Giuseppina Pieretti ◽  
Massimo Natale ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Immune Response ◽  
Extracellular Matrix ◽  
Quantitative Determination ◽  
Glycan Structure ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Plasma Haptoglobin

AbstractHaptoglobin is an acute phase glycoprotein, secreted by hepatocytes and other types of cells including keratinocytes. Haptoglobin has been suggested to impair the immune response, inhibit gelatinases in the extracellular matrix and promote angiogenesis, but its role in psoriasis is obscure to date. Changes in haptoglobin glycan structure were observed in several diseases. The aim of this study was to investigate whether haptoglobin displays glycan variations in psoriasis. We found that the pattern of plasma haptoglobin glycoforms, following two-dimensional electrophoresis, exhibited significant quantitative differences in spot intensities between patients and controls. Quantitative and qualitative differences in glycan mass, between patients and controls, were found by mass spectrometry of glycopeptides from tryptic digests of protein isolated from both patients and controls. The number of distinct fucosylated glycoforms of peptides NLFLNHSENATAK and MVSHHNLTTGATLINEQWLLTTAK was higher in patients than in controls, but no fucosylated glycan was detected on peptide VVLHPNYSQ-VDIGLIK in either case. The number of peptides with distinct triantennary and tetraantennary glycans was higher in patients than in controls. Abundance or structure of specific glycans, which are present in haptoglobin from patients and are different or missing in normal haptoglobin, might be associated with disease activity.

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