Advances in Paenibacillus larvae and American foulbrood monitoring in honey bee colonies from Argentinean apiaries

2010 ◽  
Vol 49 (3) ◽  
pp. 287-289 ◽  
Author(s):  
Natalia Jorgelina Fernández ◽  
Liesel Brenda Gende ◽  
Martín Javier Eguaras
Keyword(s):  
Honey Bee ◽  
Paenibacillus Larvae ◽  
American Foulbrood ◽  
Bee Colonies

scholarly journals Screening of Paenibacillus Larvae Spores in Apiaries from Eastern Poland. Nationwide Survey. Part I

2012 ◽  
Vol 56 (4) ◽  
pp. 539-545 ◽  
Author(s):  
Krystyna Pohorecka ◽  
Marta Skubida ◽  
Andrzej Bober ◽  
Dagmara Zdańska
Keyword(s):  
Honey Bee ◽  
Agar Medium ◽  
Culture Method ◽  
Nationwide Survey ◽  
Sheep Blood Agar ◽  
Paenibacillus Larvae ◽  
American Foulbrood ◽  
Sheep Blood ◽  
Bee Colonies ◽  
Culture Positive

Abstract Screening of the prevalence of Paenibacillus larvae spores in honey bee colonies in apiaries from 162 districts, belonging to nine provinces was carried out during 2009-2011. The honey samples were examined by the use of a culture method. Based on the number of CFUs grown on Columbia sheep blood agar medium, the level of infection and probability of American foulbrood outbreak was estimated. Altogether, 6,510 pooled honey samples from 32,550 bee colonies located in 2,294 apiaries were collected. P. larvae was identified in 45% of the surveyed apiaries. The widest distribution of P. larvae was found in the Małopolskie province. Culture-positive honey samples were obtained for 71% of the apiaries and in a half of them, the level of spores was high. In the Warmińsko-Mazurskie province, the presence of the bacterium was detected in 58% of the apiaries. In the remaining provinces, from 26% to 47% of the apiaries were contaminated with P. larvae spores

Identification and Treatment of European Foulbrood in Honey Bee Colonies

EDIS ◽  
2020 ◽  
Vol 2020 (1) ◽  
pp. 7
Author(s):  
Catherine M. Mueller ◽  
Cameron Jack ◽  
Ashley N. Mortensen ◽  
Jamie D. Ellis
Keyword(s):  
Honey Bee ◽  
Bacterial Disease ◽  
American Foulbrood ◽  
Fact Sheet ◽  
European Foulbrood ◽  
Bee Colonies ◽  
Honey Bee Larvae

European foulbrood is a bacterial disease that affects Western honey bee larvae. It is a concern to beekeepers everywhere, though it is less serious than American foulbrood because it does not form spores, which means that it can be treated. This 7-page fact sheet written by Catherine M. Mueller, Cameron J. Jack, Ashley N. Mortensen, and Jamie Ellis and published by the UF/IFAS Entomology and Nematology Department describes the disease and explains how to identify it to help beekeepers manage their colonies effectively and prevent the spread of both American and European foulbrood.https://edis.ifas.ufl.edu/in1272

scholarly journals NewPaenibacillus larvaebacterial isolates from honey bee colonies infected with American foulbrood disease in Egypt

2014 ◽  
Vol 28 (2) ◽  
pp. 271-276 ◽  
Author(s):  
Saad Hamdy Daif Masry ◽  
Sanaa Soliman Kabeil ◽  
Elsayed Elsayed Hafez
Keyword(s):  
Honey Bee ◽  
American Foulbrood ◽  
American Foulbrood Disease ◽  
Bee Colonies

scholarly journals Paenibacillus larvae Phage Tripp Genome Has 378-Base-Pair Terminal Repeats

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
J. Abraham ◽  
A.-C. Bousquet ◽  
E. Bruff ◽  
N. Carson ◽  
A. Clark ◽  
...  
Keyword(s):  
North Carolina ◽  
Honey Bee ◽  
Base Pair ◽  
Paenibacillus Larvae ◽  
American Foulbrood ◽  
Terminal Repeats

Paenibacillus larvae bacteriophage Tripp was isolated from an American foulbrood diseased honey bee hive in North Carolina, USA. The 54,439-bp genome is 48.3% G+C, encodes 92 proteins, no tRNAs, and has 378-bp direct terminal repeats. It is currently unique in Genbank.

Effect of shaking honey bee colonies affected by American foulbrood onPaenibacillus larvae larvaespore loads

2001 ◽  
Vol 40 (2) ◽  
pp. 65-69 ◽  
Author(s):  
M L Del Hoyo ◽  
M Basualdo ◽  
A Lorenzo ◽  
M A Palacio ◽  
E M Rodriguez ◽  
...  
Keyword(s):  
Honey Bee ◽  
American Foulbrood ◽  
Bee Colonies

scholarly journals Reclassification of Paenibacillus larvae subsp. pulvifaciens and Paenibacillus larvae subsp. larvae as Paenibacillus larvae without subspecies differentiation

2006 ◽  
Vol 56 (3) ◽  
pp. 501-511 ◽  
Author(s):  
Elke Genersch ◽  
Eva Forsgren ◽  
Jaana Pentikäinen ◽  
Ainura Ashiralieva ◽  
Sandra Rauch ◽  
...  
Keyword(s):  
Honey Bee ◽  
Paenibacillus Larvae ◽  
Rrna Gene ◽  
Subspecies Differentiation ◽  
Field Isolates ◽  
Sds Page ◽  
Clear Identification ◽  
Bee Colonies ◽  
Honey Bee Larvae ◽  
Reference Strains

A polyphasic taxonomic study of the two subspecies of Paenibacillus larvae, Paenibacillus larvae subsp. larvae and Paenibacillus larvae subsp. pulvifaciens, supported the reclassification of the subspecies into one species, Paenibacillus larvae, without subspecies separation. Our conclusions are based on the analysis of six reference strains of P. larvae subsp. pulvifaciens and three reference strains and 44 field isolates of P. larvae. subsp. larvae. The latter originated from brood or honey of clinically diseased honey bee colonies or from honey of both clinically diseased and asymptomatic colonies from Sweden, Finland and Germany. Colony and spore morphology, as well as the metabolism of mannitol and salicin, did not allow a clear identification of the two subspecies and SDS-PAGE of whole-cell proteins did not support the subspecies differentiation. For genomic fingerprinting, repetitive element-PCR fingerprinting using ERIC primers and PFGE of bacterial DNA were performed. The latter method is a high-resolution DNA fingerprinting method proven to be superior to most other methods for biochemical and molecular typing and has not previously been used to characterize P. larvae. ERIC-PCR identified four different genotypes, while PFGE revealed two main clusters. One cluster included most of the P. larvae subsp. larvae field isolates, as well as all P. larvae subsp. pulvifaciens reference strains. The other cluster comprised the pigmented variants of P. larvae subsp. larvae. 16S rRNA gene sequences were determined for some strains. Finally, exposure bioassays demonstrated that reference strains of P. larvae subsp. pulvifaciens were pathogenic for honey bee larvae, producing symptoms similar to reference strains of P. larvae subsp. larvae. In comparison with the type strain for P. larvae subsp. larvae, ATCC 9545T, the P. larvae subsp. pulvifaciens strains tested were even more virulent, since they showed a shorter LT100. An emended description of the species is given.

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